Immobilized Earthworm Fibrinolytic Enzyme III-1 with Carbonyldiimidazole Activated-agarose

Title: Immobilized Earthworm Fibrinolytic Enzyme III-1 with Carbonyldiimidazole Activated-agarose

Volume: 9 Issue: 1

Author(s): Xue-Qin Wu, Cen Wu and Rong-qiao He

Affiliation:

Keywords: Earthworm fibrinolytic enzyme III-1 (EFE-III-1), carbonyl-diimidazole (CDI), CDI-activated-Sepharose, Sepharose CL-6B, serine-protease

Abstract: Earthworm fibrinolytic enzyme III-1 (EFE-III-1) was prepared to couple with cross-linking agarose activated by 1,1-Carbonyl-diimidazole (CDI) in this study. Although the activity of the immobilized protease decreased to approximately 64% of the native enzyme, the activity of EFE-III-1 coupled with the resin activated by CDI was higher than that activated by cyanogen bromide (CNBr). The immobilized protease was experimentally demonstrated to hydrolyze IgG, albumin and creatine kinase, besides fibrin(ogen) and plasmin(ogen), suggesting that EFE had a broad substrate specificity.

Cite this article as:

Wu Xue-Qin, Wu Cen and He Rong-qiao, Immobilized Earthworm Fibrinolytic Enzyme III-1 with Carbonyldiimidazole Activated-agarose, Protein & Peptide Letters 2002; 9 (1) . https://dx.doi.org/10.2174/0929866023409039

DOI https://dx.doi.org/10.2174/0929866023409039	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305