The Prefoldin of the Crenarchaeon Sulfolobus solfataricus

Title: The Prefoldin of the Crenarchaeon Sulfolobus solfataricus

Volume: 15 Issue: 10

Author(s): Anna D'Amaro, Anna Valenti, Alessandra Napoli, Mose Rossi and Maria Ciaramella

Affiliation:

Keywords: Chaperone, archaea, protein folding, protein synthesis, thermophiles

Abstract: Prefoldin is a hetero-hexameric ATP-independent chaperone, shared by eukaryotes and archaea, which binds non-native proteins preventing them from aggregation. We report the identification and characterization in vivo and in vitro of the first prefoldin from a crenarchaeon, the hyperthermophile Sulfolobus solfataricus. A functional complex was obtained either co-expressing the α- and β-prefoldin subunits in Escherichia coli, or incubating at high temperature the separately expressed subunits. In S. solfataricus, prefoldin expression and apparent molecular weight were not affected by either heat or cold shock.

Cite this article as:

D'Amaro Anna, Valenti Anna, Napoli Alessandra, Rossi Mose and Ciaramella Maria, The Prefoldin of the Crenarchaeon Sulfolobus solfataricus, Protein & Peptide Letters 2008; 15 (10) . https://dx.doi.org/10.2174/092986608786071094

DOI https://dx.doi.org/10.2174/092986608786071094	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305