Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris

Title: Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris

Volume: 8 Issue: 3

Author(s): Jia-da Li, Qiang Xu and Ke-yi Wang

Affiliation:

Keywords: C-TYPE LECTIN, PICHIA PASTORIS, Trimeresurus stejnegeri lectin (TSL), alcohol oxidase (AOX1) promoter, galactose-binding C-type, mutagenesis, alcohol oxidase (AOX1)

Abstract: Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.

Cite this article as:

Li Jia-da, Xu Qiang and Wang Ke-yi, Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris, Protein & Peptide Letters 2001; 8 (3) . https://dx.doi.org/10.2174/0929866013409463

DOI https://dx.doi.org/10.2174/0929866013409463	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305