Macromolecular Properties of Diocleinae Lectins

Title: Macromolecular Properties of Diocleinae Lectins

Volume: 8 Issue: 5

Author(s): Marcio V. Ramos, Leila M. Beltramini and Renato A. Moreira

Affiliation:

Keywords: DIOCLEINAE LECTINS, Cratylia floribunda, Canavalia brasiliensis, C. ensisformis, C. maritime, Diolcea grandiflora, D.virgata, Canavalia ensiformis, Dioclea grandiflora, Canavalia maritima

Abstract: Structural properties of six closely related lectins isolated from plants belonging from the legume group Diocleinae were studied in solution. Affinity and gel filtration chromatography of the lectins at different pH conditions demonstrated that the lectins diverge in affinity for a specific matrix mainly at the pH range 4.0 up to 6.0 and that at neutral pH they tend to form dimers. Analysis of circular dichroism and fluorescence emission spectra stressed the differences of the lectin from Cratylia floribunda seeds from that of Canavalia brasiliensis, C. ensisformis, C. maritima, Diolcea grandiflora and D.virgata. According to the results, these proteins constitute a excellent model to study fine protein structure / function relationships.

Cite this article as:

Ramos V. Marcio, Beltramini M. Leila and Moreira A. Renato, Macromolecular Properties of Diocleinae Lectins, Protein & Peptide Letters 2001; 8 (5) . https://dx.doi.org/10.2174/0929866013409193

DOI https://dx.doi.org/10.2174/0929866013409193	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305