Crystallization And Preliminary X-Ray Diffraction Study Of Riboflavin Synthase

Title: Crystallization And Preliminary X-Ray Diffraction Study Of Riboflavin Synthase

Volume: 8 Issue: 1

Author(s): Zdzislaw Wawrazak, Paul V. Viitanen, Joseph C. Calabrese and Douglas B. Jordan

Affiliation:

Keywords: RIBOFLAVIN SYNTHASE

Abstract: Escherichia coli riboflavin synthase crystallizes at 22 C in the presence of 7-10percent by volume diglyme, 20-50 mM MgCl2 and pH 7.0. In this medium diffraction quality crystals are routinely obtained within 5 h and they are stable for 10 weeks. The crystals are orthogonal in space group P212121 with unit cell dimensions of a=52.4 A , b = 62.1 A, c = 218.8 A. A 97percent complete data set was collected at 2.1 A resolution.

Cite this article as:

Wawrazak Zdzislaw, Viitanen V. Paul, Calabrese C. Joseph and Jordan B. Douglas, Crystallization And Preliminary X-Ray Diffraction Study Of Riboflavin Synthase, Protein & Peptide Letters 2001; 8 (1) . https://dx.doi.org/10.2174/0929866013409698

DOI https://dx.doi.org/10.2174/0929866013409698	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305