Selective Precipitation of Haptoglobin and α2-Macroglobulin from Human Serum Using Alocasia macrorhiza Tuber Protein

Title: Selective Precipitation of Haptoglobin and α2-Macroglobulin from Human Serum Using Alocasia macrorhiza Tuber Protein

Volume: 9 Issue: 6

Author(s): B. Shivananda Nayak, N. Jagadish Ulloor and B. Shivaraj

Affiliation:

Keywords: alocasia macrorhiza, macroglobulin, haptoglobin, alocasia protein, alocasia specific serum protein

Abstract: Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatographed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented α2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of α2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent a2-globulins.

Cite this article as:

Nayak Shivananda B., Ulloor Jagadish N. and Shivaraj B., Selective Precipitation of Haptoglobin and α2-Macroglobulin from Human Serum Using Alocasia macrorhiza Tuber Protein, Protein & Peptide Letters 2002; 9 (6) . https://dx.doi.org/10.2174/0929866023408391

DOI https://dx.doi.org/10.2174/0929866023408391	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305