Heavy Metal-Mediated Crystallization of Escherichia Coli Phytase and Analysis of Bridging Interactions

Title: Heavy Metal-Mediated Crystallization of Escherichia Coli Phytase and Analysis of Bridging Interactions

Volume: 9 Issue: 4

Author(s): Daniel Lim and Zongchao Jia

Affiliation:

Keywords: Heavy Metal, Escherichia Coli, myo-inositol

Abstract: Escherichia coli phytase is a phosphatase that catalyzes the hydrolysis of phytic acid into inorganic phosphate and myo-inositol. Two crystal forms of this enzyme were obtained in the presence of heavy metals.Crystal forms I and II were obtained with the heavy atoms CdCl2 and HgCl2 and diffracted to 1.5 Å and 2.25 Å resolution, respectively. Hg2+ and Cd2+ both acted as molecular bridge(s), linking and stabilizing E. coli phytase in the unit cell, and played a crucial role in the crystallization of phytase by bridging neighbouring symmetry related molecules.

Cite this article as:

Lim Daniel and Jia Zongchao, Heavy Metal-Mediated Crystallization of Escherichia Coli Phytase and Analysis of Bridging Interactions, Protein & Peptide Letters 2002; 9 (4) . https://dx.doi.org/10.2174/0929866023408634

DOI https://dx.doi.org/10.2174/0929866023408634	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305