Binding Potency of Peptide Fragments of Type 1 Collagen Cross-Linked N Telopeptide Measured by an Enzyme-Linked Immunosorbant Assay

Title: Binding Potency of Peptide Fragments of Type 1 Collagen Cross-Linked N Telopeptide Measured by an Enzyme-Linked Immunosorbant Assay

Volume: 9 Issue: 5

Author(s): Feng-Di T. Lung, Chiu-Heng Chen, Heuy-Yi Chen, Chien-Chung Liou and Yen-Meng Liou

Affiliation:

Keywords: osteoporosis, cross-linked N-telopeptide, type I collagen, synthetic overlapping peptides, solid phase, peptide synthesis, high performance liquid chromatography, enzyme-linked immunosorbent assay

Abstract: Osteoporosis represents a major healthcare problem affecting elderly person. Urinary level of the crosslinked N-telopeptide of type I collagen is a sensitive marker of bone resorption. Ten overlapping peptides covering the N-telopeptide of alpha-2 type I collagen were synthesized, purified, and assayed for their relative binding response to anti-type I collagen cross-linked N-telopeptide (NTX) antibody by using a competitive-inhibition enzyme-linked immunosorbent assay (ELISA). Peptides 1, 2, and 3, containing the N-terminal sequence of Ntelopeptide, showed higher binding potency than peptides 4-10, suggesting that these peptides may contain binding sites for anti-NTX antibodies, and can serve as the lead for further preparation of their antibodies in order to develop novel bioassays for monitoring the bone loss in humans.

Cite this article as:

Lung T. Feng-Di, Chen Chiu-Heng, Chen Heuy-Yi, Liou Chien-Chung and Liou Yen-Meng, Binding Potency of Peptide Fragments of Type 1 Collagen Cross-Linked N Telopeptide Measured by an Enzyme-Linked Immunosorbant Assay, Protein & Peptide Letters 2002; 9 (5) . https://dx.doi.org/10.2174/0929866023408544

DOI https://dx.doi.org/10.2174/0929866023408544	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305