Production of the Human-Beta-Defensin Using Saccharomyces cerevisiae as a Host

Title: Production of the Human-Beta-Defensin Using Saccharomyces cerevisiae as a Host

Volume: 12 Issue: 6

Author(s): Ingrid Cipakova and Eva Hostinova

Affiliation:

Keywords: antimicrobial peptide, human-beta-defensin-1 (hbd-1), s. cerevisiae ah, pvt vector, heterologous expression

Abstract: Studies of the human defensins have been hampered by the lack of a simple expression system allowing for rapid production of functional peptide forms. Here, we describe a Saccharomyces cerevisiae AH22 expression system that meets that condition. The 42 amino acid form of human β-defensin-1 was expressed under the control of the ADH1 promoter. The optimum conditions for expression were determined and the stable maintenance of the pVT103L-hBD-1 chimeric vector in the yeast population was confirmed. Expressed hBD-1 was secreted into the medium (∼55 μg l-1) and purified using cation-exchange chromatography. Isolated defensin exhibited strong bactericidal effect on Escherichia coli ML-35p. We conclude that the expression system described here will be a useful tool where readily prepared and active forms of the human defensins are needed.

Cite this article as:

Cipakova Ingrid and Hostinova Eva, Production of the Human-Beta-Defensin Using Saccharomyces cerevisiae as a Host, Protein & Peptide Letters 2005; 12 (6) . https://dx.doi.org/10.2174/0929866054395761

DOI https://dx.doi.org/10.2174/0929866054395761	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305