Using 5-Hydroxytryptophan as a Probe to Follow Protein-Protein Interactions and Protein Folding Transitions

Title: Using 5-Hydroxytryptophan as a Probe to Follow Protein-Protein Interactions and Protein Folding Transitions

Volume: 12 Issue: 3

Author(s): Fernando Correa and Chuck S. Farah

Affiliation:

Keywords: 5-hydroxytryptophan, tryptophan, fluorescence, protein structure, protein-protein interactions, protein folding

Abstract: 5-Hydroxytyptophan is a fluorescent tryptophan analog that can be incorporated into recombinant proteins expressed in E. coli and is particularly useful in studies of biological systems that involve supermolecular aggregates of more than one protein. Here we review the varied applications of 5-hydroxytryptophan to study structure, interactions, conformational change and dynamics in complex protein systems.

Cite this article as:

Correa Fernando and Farah S. Chuck, Using 5-Hydroxytryptophan as a Probe to Follow Protein-Protein Interactions and Protein Folding Transitions, Protein & Peptide Letters 2005; 12 (3) . https://dx.doi.org/10.2174/0929866053587057