Characterization of the Bacteriophage ΦKMV DNA Ligase

Title: Characterization of the Bacteriophage ΦKMV DNA Ligase

Volume: 12 Issue: 7

Author(s): R. Lavigne, B. Roucourt, K. Hertveldt and G. Volckaert

Affiliation:

Keywords: dna ligase, bacteriophage, recombinant protein, phikmv

Abstract: Gene 17 product (gp17) of the Pseudomonas aeruginosa-infecting bacteriophage phiKMV shows in silico similarity to T7 DNA ligase. In a semi-quantitative activity assay, it is shown that gp17 is a functional, ATP-dependent DNA ligase, in spite of some structural differences related to DNA-binding properties). Enzymatic activity of His6-based purified expression product was optimised (4°C at 24h for sticky end double-stranded DNA fragments) and estimated at 0.5 Weiss U/μg.

Cite this article as:

Lavigne R., Roucourt B., Hertveldt K. and Volckaert G., Characterization of the Bacteriophage ΦKMV DNA Ligase, Protein & Peptide Letters 2005; 12 (7) . https://dx.doi.org/10.2174/0929866054696127

DOI https://dx.doi.org/10.2174/0929866054696127	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305