Abstract
We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.
Keywords: gene fusion, recombinant protein, affinity tag, polymerase chain reaction (pcr), small ubiquitin-related modifier (sumo)
Related Journals
Current Protein & Peptide Science
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Frontiers in Protein and Peptide Sciences
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