Heat Shock Proteins, Substrate Specificity and Modulation of Function

Title: Heat Shock Proteins, Substrate Specificity and Modulation of Function

Volume: 13 Issue: 7

Author(s): Yusuf Tutar

Affiliation:

Keywords: Hsp (Heat shock protein), co-chaperon (Hsp40; Ydj1 and Sis1)

Abstract: Hsp70 is a universally conserved essential protein chaperone. In addition to its roles in many cellular process, Hsp70 protects cells from stress by binding partially unfolded proteins. Therefore, Hsp70 prevents protein aggregation and prion formation. Prions are infectious agents and are responsible for several fatal neurodegenerative diseases. Eukaryotic cells have several cytosolic Hsp70 isoforms, some constitutively expressed (Hsc70s), and others expressed only when cells are exposed to stress (Hsp70s). To determine which factors conferred functional specificity, we constructed hybrid Hsc/Hsp chaperones. All hybrids supported growth except those that contained the ATPase domain derived from inducible Hsp70. Thus, regulation of peptide binding by ATP hydrolysis must differ significantly between Hsc- and Hsp70 isoforms. In this work, nucleotide and peptide binding domain communication of Hsp70 proteins during their interaction with nucleotides and peptide substrates were investigated in vitro by using hybrid constructs.

Cite this article as:

Tutar Yusuf, Heat Shock Proteins, Substrate Specificity and Modulation of Function, Protein & Peptide Letters 2006; 13 (7) . https://dx.doi.org/10.2174/092986606777790593

DOI https://dx.doi.org/10.2174/092986606777790593	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305