Crystallization and Preliminary X-Ray Analysis of the Catalytic Domain of Chitinase D from Bacillus circulans WL-12

Title: Crystallization and Preliminary X-Ray Analysis of the Catalytic Domain of Chitinase D from Bacillus circulans WL-12

Volume: 13 Issue: 9

Author(s): Yuichiro Kezuka, Kenji Bando, Hajime Kobayashi, Yasuo Yonou, Toshiya Sato, Takeshi Watanabe and Takamasa Nonaka

Affiliation:

Keywords: chitinase D, catalytic domain, Bacillus circulans WL-12, crystallization, preliminary X-ray analysis

Abstract: We report here on crystallization and preliminary X-ray analysis of the catalytic domain of chitinase D from Bacillus circulans WL-12. The native crystals of this domain were found to belong to the orthorhombic space group P212121. To elucidate the structure of the catalytic domain by the multiple isomorphous replacement method, 30 kinds of derivatized crystals were prepared by soaking the native crystals into a mother liquor containing salts of heavy metal atoms. Difference Patterson maps calculated for four derivatives showed strong peaks in the Harker sections.

Cite this article as:

Kezuka Yuichiro, Bando Kenji, Kobayashi Hajime, Yonou Yasuo, Sato Toshiya, Watanabe Takeshi and Nonaka Takamasa, Crystallization and Preliminary X-Ray Analysis of the Catalytic Domain of Chitinase D from Bacillus circulans WL-12, Protein & Peptide Letters 2006; 13 (9) . https://dx.doi.org/10.2174/092986606778256108

DOI https://dx.doi.org/10.2174/092986606778256108	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305