Statistical Analysis of 15 Dimensions in the Crystallization Space for Protein-DNA Complexes

Title: Statistical Analysis of 15 Dimensions in the Crystallization Space for Protein-DNA Complexes

Volume: 13 Issue: 9

Author(s): Fakhri Saida

Affiliation:

Keywords: protein-DNA complex, crystallization, sparse-matrix

Abstract: Solving the three dimensional structure of a protein-DNA complex is a prerequisite to understand, at the atomic level, the interactions between DNA-binding proteins and their target DNA sequences. Arranging these complexes into an ordered and repetitive network (a crystal, suitable for X-Ray analysis) is a time-limiting empirical step. Although it has been suggested that the crystallization space for protein-DNA complexes is probably smaller than that of non-complexed proteins, a study presenting a detailed and updated analysis of this space is still missing. Here, we analyze the successful crystallization conditions of several hundred protein-DNA complexes and present a bias-free statistical analysis of 15 crystallization parameters that include concentration, temperature, pH, precipitants, salts, divalent cations and polyamines. Our analysis shows that some crystallization parameters are interestingly restricted into narrow intervals. These restrictions could be very helpful in the design of sparse-matrix crystallization screens that target exclusively protein-DNA complexes.

Cite this article as:

Saida Fakhri, Statistical Analysis of 15 Dimensions in the Crystallization Space for Protein-DNA Complexes, Protein & Peptide Letters 2006; 13 (9) . https://dx.doi.org/10.2174/092986606778256144

DOI https://dx.doi.org/10.2174/092986606778256144	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305