Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory

Title: Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory

Volume: 13 Issue: 5

Author(s): Bernd Kastenholz

Affiliation:

Keywords: QPNC-PAGE, GPC, NMR, ICP-MS, Quantitative analysis, Biologically-active metallochaperones, Metal cofactorcontaining proteins, Native conformation, Chemical stability, Alzheimer's disease

Abstract: The quantitative analysis of metallochaperone proteins in biofluids (e.g. blood, liquor) may be a major prerequisite for clinical investigations concerning the structure-function relationships of biologically-active metal cofactorcontaining chaperones in protein-misfolding diseases (e.g. Alzheimers or related diseases). For these purposes, a new state-of-the-art gel electrophoresis [quantitative preparative native continuous polyacrylamide gel electrophoresis procedure (QPNC-PAGE)] combined with biological mass and NMR spectrometries might essentially contribute to provide fundamental insights into the metabolisms of important metal cofactors in biological systems and the proper folding of metallochaperones in conformational diseases.

Cite this article as:

Kastenholz Bernd, Important Contributions of a New Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) Procedure for Elucidating Metal Cofactor Metabolisms in Protein-Misfolding Diseases - A Theory, Protein & Peptide Letters 2006; 13 (5) . https://dx.doi.org/10.2174/092986606776819637

DOI https://dx.doi.org/10.2174/092986606776819637	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305