Synthesis of Galacturonate Containing Disaccharides and Protein Conjugates

Title: Synthesis of Galacturonate Containing Disaccharides and Protein Conjugates

Volume: 15 Issue: 19

Author(s): Marilyne Pourcelot, Maximilien Barbier, Malena Landoni, Alicia S. Couto, Eric Grand and Jose Kovensky

Affiliation:

Keywords: Synthesis, galacturonate, glycosylation, conjugates, MALDI-MS, Deprotection, TEMPO-oxidation, Hydrogermylation, luminescence, BSA CONJUGATES, Analytical thin, 1H, 13C nuclear magnetic resonance (NMR) spectra, allyl galactopyranoside, UV-MALDI-TOF mass spectrometry analysis, phenothiazine

Abstract: The synthesis of galacturonate disaccharides has been performed from suitable protected monosaccharides. The use of orthogonal protecting groups allowed us to obtain both methyl galacturonates and galacturonic acid precursors as glycosylation donors or acceptors from a unique monosaccharide derivative. The protecting groups used allowed selective introduction of a fatty acid chain to the non-reducing end of a disaccharide. Furthermore, we explored the preparation of galacturonate conjugated to a carrier protein, and determined the number of sugar residues coupled to the protein by MALDI-MS.

Cite this article as:

Pourcelot Marilyne, Barbier Maximilien, Landoni Malena, S. Couto Alicia, Grand Eric and Kovensky Jose, Synthesis of Galacturonate Containing Disaccharides and Protein Conjugates, Current Organic Chemistry 2011; 15 (19) . https://dx.doi.org/10.2174/138527211797374706