Eph/Ephrin Membrane Proteins: A Mammalian Expression Vector pTIg- BOS-Fc Allowing Rapid Protein Purification

Bryan   W.   Day; Fiona   M.   Smith; Ke      Chen; Jennifer   K.   McCarron; Nirmitha   I.   Herath; Martin      Lackmann; Andrew   W.   Boyd

doi:10.2174/092986606775101625

Abstract

There is an urgent need for high purity, single chain, fully functional Eph/ephrin membrane proteins. This report outlines the pTIg-BOS-Fc vector and purification approach resulting in rapid increased production of fully functional single chain extracellular proteins that were isolated with high purity and used in structure-function analysis and pre-clinical studies.

Keywords: Crystallography, protein expression, purification, pTIg-BOS-Fc

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