Abstract
We report A new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed A truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and evaluated aspects of its function.
Keywords: Trichoplusia ni furin, baculovirus, Cys-rich repeated segment, Tn -5B1-4 insect cells