Engineering of Enzymes for Selective Catalysis

Title: Engineering of Enzymes for Selective Catalysis

Volume: 14 Issue: 17

Author(s): Nikhil U. Nair, Carl A. Denard and Huimin Zhao

Affiliation:

Keywords: Directed evolution, enzyme engineering, biocatalysis, selectivity, Enzymes, biocatalysts, epPCR, DNA shuffling, StEP, enantioselectivity, regioselectivity, polyketide synthases, Cytochrome P450 monooxygenases, Bacillus megaterium, FAD, (FMN), NADPH, NADH, UTP, UGPase, (UNGPase), creatine kinase, glycocyamine kinases, E.coli, B.megaterium, C3-C8, MAO-N, Pseudomonas aeruginosa, FDH, Candida boidinii, (PTDH), Pseudomonas stutzeri, TSSM, XR, FDA, HPLC, CASTing, L-methionine, hydantoinase, L-enantiomer, GeneReassembly, cytochrome P450 2B1, (C-C) bond formation, DERA, Lipitor, (NAL), BFD, Pseudomonas putida, Glycosidases, Polyketides, Type I PKSs, Type II PKSs, Type III PKSs, (TAL), FACS

Abstract: Naturally occurring enzymes are truly remarkable catalysts. However, many are not suitable for practical synthetic chemistry because of poor substrate or product selectivity. This review highlights recent advances in engineering enzymes for selective catalysis. Selected topics include altering substrate specificity, altering substrate and product selectivity, engineering enzymes that catalyze carboncarbon bond formation or carbon-oxygen bond cleavage and formation, and engineering multi-function enzymes such as polyketide synthases.

Cite this article as:

U. Nair Nikhil, A. Denard Carl and Zhao Huimin, Engineering of Enzymes for Selective Catalysis, Current Organic Chemistry 2010; 14 (17) . https://dx.doi.org/10.2174/138527210792927537