Development of a Label Free IGE Sensitive Aptasensor Based on Electrochemical Impedance Spectrometry

Title: Development of a Label Free IGE Sensitive Aptasensor Based on Electrochemical Impedance Spectrometry

Volume: 13 Issue: 7

Author(s): Pinar Kara, Buket Meric and Mehmet Ozsoz

Affiliation:

Keywords: Aptamer, IgE, biosensor, electrochemical impedance spectrometry (EIS)

Abstract: A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5 Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analyses are based on electron transfer resistance (Rct) in the presence of 5mM [Fe(CN)6]3-/4-.

Cite this article as:

Kara Pinar, Meric Buket and Ozsoz Mehmet, Development of a Label Free IGE Sensitive Aptasensor Based on Electrochemical Impedance Spectrometry, Combinatorial Chemistry & High Throughput Screening 2010; 13 (7) . https://dx.doi.org/10.2174/1386207311004070578

DOI https://dx.doi.org/10.2174/1386207311004070578	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402