Abstract
The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
Keywords: Cell-free protein synthesis, in vitro translation, chaperone, disulfide bond formation, in vitro selection, unnatural amino acid
Current Pharmaceutical Biotechnology
Title: A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research
Volume: 11 Issue: 3
Author(s): Hiroyuki Ohashi, Takashi Kanamori, Yoshihiro Shimizu and Takuya Ueda
Affiliation:
Keywords: Cell-free protein synthesis, in vitro translation, chaperone, disulfide bond formation, in vitro selection, unnatural amino acid
Abstract: The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.
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Cite this article as:
Ohashi Hiroyuki, Kanamori Takashi, Shimizu Yoshihiro and Ueda Takuya, A Highly Controllable Reconstituted Cell-Free System -a Breakthrough in Protein Synthesis Research, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791111889
DOI https://dx.doi.org/10.2174/138920110791111889 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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