Abstract
Objective: In order to control the quality better, this study aimed to develop two bioassay methods of Banlangen Granule (BLGG) based on its anti-influenza activity and antiinflammatory activity and to verify the necessity of established methods by relating the results tested by chemical methods.
Methods: First, the bioassay methods for determining the biopotency of the anti-influenza effect and anti-inflammatory activity were established and applied, taking neuraminidase and cyclooxygenase- 2 as disease targets, respectively. Secondly, the ultra-high-performance liquid chromatography coupled photo-diode array detector (UPLC-PDA) technique was used to perform fingerprints and quantify chemical compounds. Finally, the correlation analysis was performed on the results of bioassay methods and chemical methods to assist in choosing the effective quality markers for the BLGG.
Results: Two accurate, stable, and repeatable bioassay methods were developed and applied to the determination of 57 batches of samples. The chemical fingerprints and contents of seven quality compounds were obtained based on UPLC-PDA methods. From the results of correlation analysis, the highest intensity correlation between these quality markers was medium with a r=0.495 (P<0.01), which indicates the need for establishing a bioassay method for BLGG.
Conclusion: This present work illuminated that bioassay methods can be a great means to evaluate the quality of BLGG effectively and also provided a paradigm case for the quality control of other traditional Chinese medicine preparations.
Graphical Abstract
[1]
Wu, L.; Chen, Y.L.; Ma, Y.F.; Yang, Z.Y.; Yang, N.; Deng, W.Z.; Chen, Y.B.; Sun, Y.Y.; Lin, Y.M.; Lin, L. Clinical practice guideline on treating influenza in adult patients with Chinese patent medicines. Pharm. Res., 2020, 160, 105101.
[http://dx.doi.org/10.1016/j.phrs.2020.105101]
[http://dx.doi.org/10.1016/j.phrs.2020.105101]
[2]
Zhang, D.; Zhang, B.; Lv, J.T.; Sa, R.N.; Zhang, X.M.; Lin, Z.J. The clinical benefits of Chinese patent medicines against COVID-19 based on current evidence. Pharm. Res., 2020, 157, 104882.
[http://dx.doi.org/10.1016/j.phrs.2020.104882]
[http://dx.doi.org/10.1016/j.phrs.2020.104882]
[3]
Zhu, Q.J.; Xu, W.J.; Li, X.S. Acupuncture combined with traditional Chinese medicine preparation for the treatment of marrow suppression after chemotherapy A protocol for systematic review and meta-analysis. Medicine, 2021, 100(43), 27646.
[http://dx.doi.org/10.1097/MD.0000000000027646]
[http://dx.doi.org/10.1097/MD.0000000000027646]
[4]
Liu, H.X.; Wang, S.R.; Lei, Y.; Shang, J.J. Characteristics and advantages of Traditional Chinese Medicine in the treatment of acute myocardial infarction. J. Tradit. Chin, 2011, 31(4), 269-272.
[http://dx.doi.org/10.1016/S0254-6272(12)60002-8]
[http://dx.doi.org/10.1016/S0254-6272(12)60002-8]
[5]
Zhou, M.E.; Jin, W.Q.; Li, P.; Wang, R.L.; Guo, X.T. Traditional chinese medicine in the treatment of hemorrhoids-a review of preparations used and their mechanism of action. Front. Pharm, 2023, 14.
[http://dx.doi.org/10.3389/fphar.2023.1270339]
[http://dx.doi.org/10.3389/fphar.2023.1270339]
[6]
Zhang, R.F.; Nie, Y.W.; Wang, Y.; Zhai, X.X.; Zhu, J.Y.; Duan, Y.J. Effectiveness of traditional Chinese medicine preparations for facial seborrheic dermatitis: Case reports. Heliyon, 2022, 8(12), 12338.
[http://dx.doi.org/10.1016/j.heliyon.2022.e12338]
[http://dx.doi.org/10.1016/j.heliyon.2022.e12338]
[7]
Lin, Y.L.; Cai, C.Z.; Alias, H.L.; Wong, P.; Hu, Z.J. A cross-sectional survey of self-medication with Traditional Chinese Medicine for treatment and prevention of COVID-19. Complemen. Ther. Med., 2022, 77, 102898.
[http://dx.doi.org/10.1016/j.ctim.2022.102898]
[http://dx.doi.org/10.1016/j.ctim.2022.102898]
[8]
Lu, C.L.; Zheng, R.X.; Xue, X.; Zhang, X.W.; Liu, X.H.; Jin, X.Y.; Pu, F.L.; Lan, H.D.; Fang, M.; Kong, L.Y.; Willcox, M.; Graz, B.; Houriet, J.; Hu, X.Y. Traditional Chinese medicine for COVID-19 pandemic and emerging challenges: An online cross-sectional survey in China. Integr. Med. Res, 2021, 10, 100798.
[http://dx.doi.org/10.1016/j.imr.2021.100798]
[http://dx.doi.org/10.1016/j.imr.2021.100798]
[9]
Xiao, P.; Ye, W.Y.; Chen, J.W.; Li, X. Antiviral activities against influenza virus (FM1) of bioactive fractions and representative compounds extracted from Banlangen. J. Trad. Chin. Med., 2016, 36(3), 369-376.
[http://dx.doi.org/10.1016/S0254-6272(16)30051-6]
[http://dx.doi.org/10.1016/S0254-6272(16)30051-6]
[10]
Nie, L.X.; Wu, Y.L.; Dai, Z.; Ma, S.C. Antiviral activity of Isatidis Radix derived glucosinolate isomers and their breakdown products against influenza A in vitro/ovo and mechanism of action. J. Ethnopharm., 2020, 251, 112550.
[http://dx.doi.org/10.1016/j.jep.2020.112550]
[http://dx.doi.org/10.1016/j.jep.2020.112550]
[11]
Chen, Y.; Wu, C.J.; Li, H.F.; Powell, H.; Chen, A.; Zhu, G.D.; Cong, W.H.; Fu, L.; Pekosz, A.; Leng, S.X. Antiviral effect and mechanism of Phillyrin and its reformulated FS21 against influenza. Influenza. Other. Resp., 2023, 17(3), 13112.
[http://dx.doi.org/10.1111/irv.13112]
[http://dx.doi.org/10.1111/irv.13112]
[12]
Lowy, R.J. Influenza virus induction of apoptosis by intrinsic and extrinsic mechanisms. Int. Rev. Immunol, 2003, 22(5-6), 425-449.
[http://dx.doi.org/10.1080/08830180305216]
[http://dx.doi.org/10.1080/08830180305216]
[13]
McKimm-Breschkin, J.L. Influenza neuraminidase inhibitors: Antiviral action and mechanisms of resistance. Influenza. Other. Resp, 2013, 7(25-36), 12047.
[http://dx.doi.org/10.1111/irv.12047]
[http://dx.doi.org/10.1111/irv.12047]
[14]
Han, J.; Perez, J.; Schafer, A.; Cheng, H.; Peet, N.; Rong, L.J.; Manicassamy, B. Influenza virus: Small molecule therapeutics and mechanisms of antiviral resistance. Curr. Med. Chin, 2018, 25(38), 5115-5127.
[http://dx.doi.org/10.2174/0929867324666170920165926]
[http://dx.doi.org/10.2174/0929867324666170920165926]
[15]
Chen, J.; Zhu, Z.P.; Gao, T.H.; Chen, Y.; Yang, Q.S.; Fu, C.M.; Zhu, Y.N.; Wang, F.; Liao, W. Isatidis radix and isatidis folium: A systematic review on ethnopharmacology, phytochemistry and pharmacology. J. Ethnopharm, 2022, 283, 114648.
[http://dx.doi.org/10.1016/j.jep.2021.114648]
[http://dx.doi.org/10.1016/j.jep.2021.114648]
[16]
Bo, T.; Nie, W.M.; Ding, P.P.; Li, F.Y.; Chen, W.W.; Zhou, Z.P.; Zhang, X.; Fan, R.; Huo, D.D.; Zhao, M. Efficacy of treatment of influenza A (H1N1) with oseltamivir phosphate and isatis root granules. Med. J. Chin. People's. Armed. Police. Forces, 2013, 24(6), 456-470.
[http://dx.doi.org/10.14010/j.cnki.wjyx.2013.06.018]
[http://dx.doi.org/10.14010/j.cnki.wjyx.2013.06.018]
[17]
Xiao, P.; Huang, H.; Chen, J.; Li, X. In vitro antioxidant and anti-inflammatory activities of Radix Isatidis extract and bioaccessibility of six bioactive compounds after simulated gastro-intestinal digestion. J. Ethnopharmacol., 2014, 157, 55-61.
[http://dx.doi.org/10.1016/j.jep.2014.09.005] [PMID: 25256688]
[http://dx.doi.org/10.1016/j.jep.2014.09.005] [PMID: 25256688]
[18]
Zang, Y.; Miao, Y.; Wu, T.; Cheng, Z. Development of a thin-layer chromatography bioautographic assay for neuraminidase inhibitors hyphenated with electrostatic field induced spray ionisation-mass spectrometry for identification of active Isatis indigotica root compounds. J. Chromatogr. A, 2021, 1638, 461597.
[http://dx.doi.org/10.1016/j.chroma.2020.461597] [PMID: 33250163]
[http://dx.doi.org/10.1016/j.chroma.2020.461597] [PMID: 33250163]
[19]
Wang, X.; Xie, Y.; Hu, X.; Li, Y.; Hu, P.; Wang, Y.; Liang, Q.; Luo, G. Qualitative and quantitative analysis of glucosinolates and nucleosides in radix isatidis by HPLC and liquid chromatography tandem mass spectrometry. Acta Pharm. Sin. B, 2013, 3(5), 337-344.
[http://dx.doi.org/10.1016/j.apsb.2013.08.002] [PMID: 32288995]
[http://dx.doi.org/10.1016/j.apsb.2013.08.002] [PMID: 32288995]
[20]
Wang, J.M.; Li, W. Determination of effective components in Banlangen Granules and research progress of pharmacological effects. Chin. Med. Herald, 2019, 16(18), 49-52.
[21]
Wang, X.M.; Xie, Y.Y.; Hu, X.M.; Li, Y.K.; Hu, P.; Wang, Y.M.; Liang, Q.L.; Luo, G.A. Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry. APSB, 2013, 3(5), 337-344.
[http://dx.doi.org/10.1016/j.apsb.2013.08.002]
[http://dx.doi.org/10.1016/j.apsb.2013.08.002]
[22]
Pharmacopoeia of people’s republic of china, china medical science press. 2020. Available from: https://db.ouryao.com/yd2020/
[23]
Shen, M.R.; He, Y.; Shi, S.M. Development of chromatographic technologies for the quality control of traditional chinese medicine in the chinese pharmacopoeia. J. Pharm. Anal., 2021, 11(2), 155-162.
[http://dx.doi.org/10.1016/j.jpha.2020.11.008] [PMID: 34012691]
[http://dx.doi.org/10.1016/j.jpha.2020.11.008] [PMID: 34012691]
[24]
Kong, W.J.; Zhao, Y.L.; Shan, L.M.; Xiao, X.H.; Guo, W.Y. Investigation on the spectrum-effect relationships of EtOAc extract from Radix Isatidis based on HPLC fingerprints and microcalorimetry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2008, 871(1), 109-114.
[http://dx.doi.org/10.1016/j.jchromb.2008.06.053] [PMID: 18639503]
[http://dx.doi.org/10.1016/j.jchromb.2008.06.053] [PMID: 18639503]
[25]
Liu, X.; Jiang, W.; Su, M.; Sun, Y.; Liu, H.; Nie, L.; Zang, H. Quality evaluation of traditional Chinese medicines based on fingerprinting. J. Sep. Sci., 2020, 43(1), 6-17.
[http://dx.doi.org/10.1002/jssc.201900365] [PMID: 31282117]
[http://dx.doi.org/10.1002/jssc.201900365] [PMID: 31282117]
[26]
Wang, Q.; Zou, Z.; Zhang, Y.; Lin, P.; Lan, T.; Qin, Z.; Xu, D.; Wu, H.; Yao, Z. Characterization of chemical profile and quantification of major representative components of Wendan decoction, a classical traditional Chinese medicine formula. J. Sep. Sci., 2021, 44(5), 1036-1061.
[http://dx.doi.org/10.1002/jssc.202000952] [PMID: 33403778]
[http://dx.doi.org/10.1002/jssc.202000952] [PMID: 33403778]
[27]
Zhang, Q.; Shi, Q.N.; Li, L.; Li, L.J.; Su, Y.; Guo, Y.L. Development of thin- layer chromatography carbon fiber ionization mass spectrometry and its application in analysis of traditional chinese medicine. Chin. J. Anal. Chem., 2022, 50(9), 1345-1354.
[http://dx.doi.org/10.19756/j.issn.0253-3820.210868]
[http://dx.doi.org/10.19756/j.issn.0253-3820.210868]
[28]
Yu, W.T.; Luo, M.; Wu, H.; Li, J.M.; Yang, S.L.; Zhang, W.G.; Yao, M.; Feng, Y.L. The quality evaluation system of a famous traditional Chinese medicine Huaganjian decoction was established. Biomed. Chromatogr., 2023.
[http://dx.doi.org/10.1002/bmc.5752]
[http://dx.doi.org/10.1002/bmc.5752]
[29]
Lin, P.; Wang, Q.; Liu, Y.; Qin, Z.; Gao, H.; Ye, M.; Shang, H.; Yao, X.; Yao, Z. Characterization of chemical profile and quantification of representative components of DanLou tablet, a traditional Chinese medicine prescription, by UHPLC-Q/TOF-MS combined with UHPLC-TQ-MS. J. Pharm. Biomed. Anal., 2020, 180, 113070.
[http://dx.doi.org/10.1016/j.jpba.2019.113070] [PMID: 31911285]
[http://dx.doi.org/10.1016/j.jpba.2019.113070] [PMID: 31911285]
[30]
Seo, C.S.; Kim, O.S.; Kim, J.H.; Shin, H.K. Simultaneous quantification and antiatherosclerosis effect of the traditional Korean medicine, Hwangryunhaedok-tang. Bmc. Complem. Altern.Med, 2015, 15(1), 108.
[http://dx.doi.org/10.1186/s12906-015-0632-5]
[http://dx.doi.org/10.1186/s12906-015-0632-5]
[31]
Pirog, A.; Faktor, J.; Urban-Wojciuk, Z.; Kote, S.; Chruściel, E.; Arcimowicz, Ł.; Marek-Trzonkowska, N.; Vojtesek, B.; Hupp, T.R.; Al Shboul, S.; Brennan, P.M.; Smoleński, R.T.; Goodlett, D.R.; Dapic, I. Comparison of different digestion methods for proteomic analysis of isolated cells and FFPE tissue samples. Talanta, 2021, 233, 122568.
[http://dx.doi.org/10.1016/j.talanta.2021.122568] [PMID: 34215064]
[http://dx.doi.org/10.1016/j.talanta.2021.122568] [PMID: 34215064]
[32]
Zhu, Y.D.; Pang, H.L.; Zhou, Q.H.; Qin, Z.F.; Jin, Q.; Finel, M.; Wang, Y.N.; Qin, W.W.; Lu, Y.; Wang, D.D.; Ge, G.B. An ultra-sensitive and easy-to-use assay for sensing human UGT1A1 activities in biological systems. J. Pharm. Anal., 2020, 10(3), 263-270.
[http://dx.doi.org/10.1016/j.jpha.2020.05.005] [PMID: 32612873]
[http://dx.doi.org/10.1016/j.jpha.2020.05.005] [PMID: 32612873]
[33]
Alnafisi, A.; Hughes, J.; Wang, G.D.; Miller, C.A. Evaluating polycyclic aromatic hydrocarbons using a yeast bioassay. Environ. Toxical. Chem, 2007, 26(7), 1333-1339.
[http://dx.doi.org/10.1897/06-433R.1]
[http://dx.doi.org/10.1897/06-433R.1]
[34]
Shiizaki, K.; Goto, K.; Ishige, A.; Komatsu, Y. Bioassay of phytoestrogen in herbal medicine used for postmenopausal disorder using transformed MCF-7 cells. Phytother. Res, 1999, 13(6), 489-503.
[http://dx.doi.org/10.1002/(SICI)1099-1573(199909)13:6<498::AID-PTR495>3.0.CO;2-J]
[http://dx.doi.org/10.1002/(SICI)1099-1573(199909)13:6<498::AID-PTR495>3.0.CO;2-J]
[35]
Chen, G.; Liu, F.; Zhang, S.; You, T.; Wen, L.; Wei, Q. Bioassay- and liquid chromatography/mass spectrometry-guided acetylcholinesterase inhibitors from Picriafel-terrae. Pharmacogn. Mag., 2013, 9(36)(Suppl. 1), 25.
[http://dx.doi.org/10.4103/0973-1296.117857] [PMID: 24143041]
[http://dx.doi.org/10.4103/0973-1296.117857] [PMID: 24143041]
[36]
Shu, X.; Yu, L.; Tang, Y.; Zhang, L.; Ding, A.; Luo, D.; Duan, J.; Shen, X. Bioassay-guided separation of the proinflammatory constituents from the roots of Euphorbia kansui. J. Nat. Med., 2010, 64(1), 98-103.
[http://dx.doi.org/10.1007/s11418-009-0366-0] [PMID: 19844773]
[http://dx.doi.org/10.1007/s11418-009-0366-0] [PMID: 19844773]
[37]
Grilo, L.F.; Martins, J.D.; Cavallaro, C.H.; Nathanielsz, P.W.; Oliveira, P.J.; Pereira, S.P. Development of a 96-well based assay for kinetic determination of catalase enzymatic-activity in biological samples. Toxicol. In Vitro, 2020, 69, 104996.
[http://dx.doi.org/10.1016/j.tiv.2020.104996] [PMID: 32898619]
[http://dx.doi.org/10.1016/j.tiv.2020.104996] [PMID: 32898619]
[38]
Muluye, R.A.; Bian, Y.H.; Alemu, P.N. Anti-inflammatory and antimicrobial effects of heatclearing Chinese herbs: A current review. J. Tradit., 2014, 4, 93-98.
[http://dx.doi.org/10.4103/2225-4110.126635]
[http://dx.doi.org/10.4103/2225-4110.126635]
[39]
Qian, X.; Nie, L.; Zhao, H.; Dai, Z.; Ma, S.; Liu, J.; Kuang, Y. Discovery and molecular elucidation of the anti-influenza material basis of Banlangen granules based on biological activities and ultra-high performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry. J. Ethnopharmacol., 2022, 298, 115683.
[http://dx.doi.org/10.1016/j.jep.2022.115683] [PMID: 36057409]
[http://dx.doi.org/10.1016/j.jep.2022.115683] [PMID: 36057409]
[40]
Liu, S.; Yan, J.; Xing, J.P.; Song, F.R.; Liu, Z.Q.; Liu, S.Y. Characterization of compounds and potential neuraminidase inhibitors from the n-butanol extract of Compound Indigowoad Root Granule using ultrafiltration and liquid chromatography–tandem mass spectrometry. JPBA, 2012, 59, 96-101.
[http://dx.doi.org/10.1016/j.jpba.2011.10.015]
[http://dx.doi.org/10.1016/j.jpba.2011.10.015]
[41]
Han, H.B.; Yan, D.; Wang, J.B.; Wang, Y.J.; Zhu, Z.C.; Wei, L.; Xiao, X.H. Biological evaluation of Radix Isatidis based on neuraminidase activity assay. APSB, 2009, 44(2), 162-166.
[http://dx.doi.org/10.16438/j.0513-4870.2009.02.007]
[http://dx.doi.org/10.16438/j.0513-4870.2009.02.007]
[42]
Zhang, L.; He, Y.; Ma, Z.W. Study on quality control of radix isatidis based on antiviral activity detection. Capit. Med, 2023, 30(15), 155-156.
[http://dx.doi.org/10.3969/j.issn.1005-8257.2023.15.052]
[http://dx.doi.org/10.3969/j.issn.1005-8257.2023.15.052]
[43]
Tang, H.Y.; Yan, D.; Zhang, S.F.; Li, H.B.; Liu, R.H.; Xiao, X.H. Agglutinated activity bioassay method for the determination of antivirus potency of Banlangen granula. Acta. Pharm. Sin, 2010, 45(4), 479-483.
[http://dx.doi.org/10.16438/j.0513-4870.2010.04.007]
[http://dx.doi.org/10.16438/j.0513-4870.2010.04.007]
[44]
Tan, M.R.; Yan, D.; Qiu, L.L.; Chen, L.H.; Yan, Y.; Jin, C.; Li, H.B.; Xiao, X.H. Investigation on production process quality control of traditional Chinese medicine-Banlangen granule as an example. Chin. J. Chin. Mater. Med., 2012, 37(8), 1122-1126.
[http://dx.doi.org/10.4268/cjcmm20120815]
[http://dx.doi.org/10.4268/cjcmm20120815]
[45]
Klenow, L.; Elfageih, R.; Gao, J.; Wan, H.; Withers, S.G.; de Gier, J.W.; Daniels, R. Influenza virus and pneumococcal neuraminidases enhance catalysis by similar yet distinct sialic acid–binding strategies. J. Biol. Chem., 2023, 299(2), 102891.
[http://dx.doi.org/10.1016/j.jbc.2023.102891] [PMID: 36634846]
[http://dx.doi.org/10.1016/j.jbc.2023.102891] [PMID: 36634846]
[46]
Air, G.M. Influenza neuraminidase. Influenza. Other. Resp, 2012, 6(4), 245-256.
[http://dx.doi.org/10.1111/j.1750-2659.2011.00304.x]
[http://dx.doi.org/10.1111/j.1750-2659.2011.00304.x]
[47]
Colman, P.M. Neuraminidase inhibitors as antivirals. Vaccine, 2002, 20, 55-58.
[http://dx.doi.org/10.1016/S0264-410X(02)00132-9]
[http://dx.doi.org/10.1016/S0264-410X(02)00132-9]
[48]
Chen, Z.; Wang, J.; Yuan, J.; Wang, Z.; Tu, Z.; Crommen, J.; Luo, W.; Guo, J.; Zhang, T.; Jiang, Z. Rapid screening of neuraminidase inhibitors using an at-line nanofractionation platform involving parallel oseltamivir-sensitive/resistant neuraminidase bioassays. J. Chromatogr. A, 2023, 1687, 463693.
[http://dx.doi.org/10.1016/j.chroma.2022.463693] [PMID: 36516530]
[http://dx.doi.org/10.1016/j.chroma.2022.463693] [PMID: 36516530]
[49]
Jia, R.; Zhang, J.; Shi, F.; Bonomini, A.; Lucca, C.; Bertagnin, C.; Zhang, J.; Liu, C.; Jia, H.; Jiang, Y.; Ma, X.; Loregian, A.; Huang, B.; Zhan, P.; Liu, X. Discovery of N-substituted oseltamivir derivatives as novel neuraminidase inhibitors with improved drug resistance profiles and favorable drug-like properties. Eur. J. Med. Chem., 2023, 252, 115275.
[http://dx.doi.org/10.1016/j.ejmech.2023.115275] [PMID: 36931117]
[http://dx.doi.org/10.1016/j.ejmech.2023.115275] [PMID: 36931117]
[50]
Singh, N.; Anjum, N.; Chandra, R. Combating influenza: Natural products as neuraminidase inhibitors. Phytochem. Rev., 2019, 18, 69-107.
[http://dx.doi.org/10.1007/s11101-018-9581-1]
[http://dx.doi.org/10.1007/s11101-018-9581-1]
[51]
McNicholl, I.R.; McNicholl, J.J. Neuraminidase inhibitors: Zanamivir and oseltamivir. Aun. Pharmacother, 2001, 35(1), 57-70.
[http://dx.doi.org/10.1345/aph.10118]
[http://dx.doi.org/10.1345/aph.10118]
[52]
Tian, L.; Wang, Z.; Wu, H.; Wang, S.; Wang, Y.; Wang, Y.; Xu, J.; Wang, L.; Qi, F.; Fang, M.; Yu, D.; Fang, X. Evaluation of the anti-neuraminidase activity of the traditional Chinese medicines and determination of the anti-influenza A virus effects of the neuraminidase inhibitory TCMs in vitro and in vivo. J. Ethnopharmacol., 2011, 137(1), 534-542.
[http://dx.doi.org/10.1016/j.jep.2011.06.002] [PMID: 21699971]
[http://dx.doi.org/10.1016/j.jep.2011.06.002] [PMID: 21699971]
[53]
Li, H.B.; Yan, D.; Wang, J.B.; Wang, J.Y.; Bei, Z.C.; Wei, L.; Xiao, X.H. [Biological evaluation of Radix Isatidis based on neuraminidase activity assay]. Yao Xue Xue Bao, 2009, 44(2), 162-166.
[http://dx.doi.org/10.16438/j.0513-4870.2009.02.007] [PMID: 19408687]
[http://dx.doi.org/10.16438/j.0513-4870.2009.02.007] [PMID: 19408687]
[54]
Pang, Y.; Liu, X.; Zhao, C.; Shi, X.; Zhang, J.; Zhou, T.; Xiong, H.; Gao, X.; Zhao, X.; Yang, X.; Ning, G.; Zhang, X.; Feng, S.; Yao, X. LC−MS/MS-based arachidonic acid metabolomics in acute spinal cord injury reveals the upregulation of 5-LOX and COX-2 products. Free Radic. Biol. Med., 2022, 193(Pt 1), 363-372.
[http://dx.doi.org/10.1016/j.freeradbiomed.2022.10.303] [PMID: 36272669]
[http://dx.doi.org/10.1016/j.freeradbiomed.2022.10.303] [PMID: 36272669]
[55]
Harris, R.C. An update on cyclooxygenase-2 expression and metabolites in the kidney. Curr. Opin. Nephrol. Hypertens., 2008, 17(1), 64-69.
[http://dx.doi.org/10.1097/MNH.0b013e3282f1bb7d] [PMID: 18090672]
[http://dx.doi.org/10.1097/MNH.0b013e3282f1bb7d] [PMID: 18090672]
[56]
Fujimoto, Y.; Yonemura, T.; Sakuma, S. Role of linoleic acid hydroperoxide preformed by Cyclooxygenase-1 or-2 on the regulation of prostaglandin formation from arachidonic acid by the respective enzyme. J. Clin. Biochem. Nutr, 2008, 43(2), 65-68.
[http://dx.doi.org/10.3164/jcbn.2008047]
[http://dx.doi.org/10.3164/jcbn.2008047]
[57]
Yasir Khan, H.; Parveen, S.; Yousuf, I.; Tabassum, S.; Arjmand, F. Metal complexes of NSAIDs as potent anti-tumor chemotherapeutics: Mechanistic insights into cytotoxic activity via multiple pathways primarily by inhibition of COX–1 and COX–2 enzymes. Coord. Chem. Rev., 2022, 453, 214316.
[http://dx.doi.org/10.1016/j.ccr.2021.214316]
[http://dx.doi.org/10.1016/j.ccr.2021.214316]
[58]
Uehara, Y.; Murata, Y.; Shiga, S.; Hosoi, Y. NSAIDs diclofenac, indomethacin, and meloxicam highly upregulate expression of ICAM-1 and COX-2 induced by X-irradiation in human endothelial cells. Biochem. Biophys. Res. Commun., 2016, 479(4), 847-852.
[http://dx.doi.org/10.1016/j.bbrc.2016.09.120] [PMID: 27687548]
[http://dx.doi.org/10.1016/j.bbrc.2016.09.120] [PMID: 27687548]
[59]
Mukhopadhyay, N.; Shukla, A.; Makhal, P.N.; Kaki, V.R. Natural product-driven dual COX-LOX inhibitors: Overview of recent studies on the development of novel anti-inflammatory agents. Heliyon, 2023, 9(3), e14569.
[http://dx.doi.org/10.1016/j.heliyon.2023.e14569] [PMID: 37020932]
[http://dx.doi.org/10.1016/j.heliyon.2023.e14569] [PMID: 37020932]
[60]
Antoniou, K.; Malamas, M.; Drosos, A.A. Clinical pharmacology of celecoxib, a COX-2 selective inhibitor. Expert Opin. Pharmacother., 2007, 8(11), 1719-1732.
[http://dx.doi.org/10.1517/14656566.8.11.1719] [PMID: 17685888]
[http://dx.doi.org/10.1517/14656566.8.11.1719] [PMID: 17685888]
[61]
Miyamoto, K.; Miyake, S.; Mizuno, M.; Oka, N.; Kusunoki, S.; Yamamura, T. Selective COX-2 inhibitor celecoxib prevents experimental autoimmune encephalomyelitis through COX-2-independent pathway. Brain, 2006, 129(8), 1984-1992.
[http://dx.doi.org/10.1093/brain/awl170] [PMID: 16835249]
[http://dx.doi.org/10.1093/brain/awl170] [PMID: 16835249]
[62]
Han, S.; Roman, J. COX-2 inhibitors suppress lung cancer cell growth by inducing p21 via COX-2 independent signals. Lung Cancer, 2006, 51(3), 283-296.
[http://dx.doi.org/10.1016/j.lungcan.2005.10.015] [PMID: 16376453]
[http://dx.doi.org/10.1016/j.lungcan.2005.10.015] [PMID: 16376453]
[63]
Zarghi, A.; Arfaei, S. Selective COX-2 Inhibitors: A review of their structure-activity relationships. Iran. J. Pharm. Res., 2011, 10(4), 655-683.
[PMID: 24250402]
[PMID: 24250402]
[64]
Kyme, H.; Lee, C.T.; Kim, Y.T.; Lee, J.H. Self-enzyme chemiluminescence immunoassay capable of rapidly diagnosing the infection of influenza A (H1N1) virus. Talanta, 2019, 192, 189-196.
[http://dx.doi.org/10.1016/j.talanta.2018.09.049] [PMID: 30348377]
[http://dx.doi.org/10.1016/j.talanta.2018.09.049] [PMID: 30348377]
[65]
Walters, K.M.; Woessner, K.M. An overview of nonsteroidal antiinflammatory drug reactions. Immunol. Allergy Clin. North Am., 2016, 36(4), 625-641.
[http://dx.doi.org/10.1016/j.iac.2016.06.001] [PMID: 27712759]
[http://dx.doi.org/10.1016/j.iac.2016.06.001] [PMID: 27712759]
[66]
Ren, J.; Zhang, A.H.; Kong, L.; Han, Y.; Yan, G.L.; Sun, H.; Wang, X.J. Analytical strategies for the discovery and validation of quality-markers of traditional Chinese medicine. Phytomedicine, 2020, 67, 153165.
[http://dx.doi.org/10.1016/j.phymed.2019.153165] [PMID: 31954259]
[http://dx.doi.org/10.1016/j.phymed.2019.153165] [PMID: 31954259]
[67]
Zhang, H.; Zhang, Y.; Zhang, T.; Liu, C. Research progress on quality markers of traditional Chinese medicine. J. Pharm. Biomed. Anal., 2022, 211, 114588.
[http://dx.doi.org/10.1016/j.jpba.2022.114588] [PMID: 35091155]
[http://dx.doi.org/10.1016/j.jpba.2022.114588] [PMID: 35091155]
[68]
Chen, S.; Yang, X.; Wei, Z.; Zhang, Y.; Huang, Y.; Shi, Z.; Zhang, Z.; Wang, J.; Zhang, H.; Ma, J.; Xiao, X.; Niu, M. Establishment of an anti-inflammation-based bioassay for the quality control of the 13-component TCM formula (Lianhua Qingwen). Pharm. Biol., 2021, 59(1), 535-543.
[http://dx.doi.org/10.1080/13880209.2021.1917627] [PMID: 33941036]
[http://dx.doi.org/10.1080/13880209.2021.1917627] [PMID: 33941036]
[69]
Shi, S.; Li, K.; Peng, J.; Li, J.; Luo, L.; Liu, M.; Chen, Y.; Xiang, Z.; Xiong, P.; Liu, L.; Cai, W. Chemical characterization of extracts of leaves of Kadsua coccinea (Lem.) A.C. Sm. by UHPLC-Q-Exactive Orbitrap Mass spectrometry and assessment of their antioxidant and anti-inflammatory activities. Biomed. Pharmacother., 2022, 149, 112828.
[http://dx.doi.org/10.1016/j.biopha.2022.112828] [PMID: 35339830]
[http://dx.doi.org/10.1016/j.biopha.2022.112828] [PMID: 35339830]
[70]
Chen, J.X.; Ma, X.L.; Zhao, H.H.; Yang, Y.; Han, J.; Guo, S.Z.; Liu, B.; Ni, J.; Wang, W. Biological effects based quality control of a traditional Chinese medicine. J. Med. Plants Res., 2022, 5(31), 6895-6906.http://www.academicjournals.org/JMPR
[71]
Chen, Q.L.; Zhu, L.; Tang, Y.N.; Kwan, H.Y.; Zhao, Z.Z.; Chen, H.B.; Yi, T. Comparative evaluation of chemical profiles of three representative ‘snow lotus’ herbs by UPLC‐DAD‐QTOF‐MS combined with principal component and hierarchical cluster analyses. Drug Test. Anal., 2017, 9(8), 1105-1115.
[http://dx.doi.org/10.1002/dta.2123] [PMID: 27764538]
[http://dx.doi.org/10.1002/dta.2123] [PMID: 27764538]
[72]
Li, C.; Li, F.; Ye, H.T. Molecular quantification, a new strategy for quality control of Chinese patent medicine containing animal-derived crude drug: Qi She in Jinlong capsule as an example. JPBA, 2022, 207(5), 114428.
[http://dx.doi.org/10.1016/j.jpba.2021.114428]
[http://dx.doi.org/10.1016/j.jpba.2021.114428]
[73]
Sun, C.; Gao, M.; Qiao, M. Research progress of traditional Chinese medicine compound “Xiaochaihu Decoction” in the treatment of depression. Biomed. Pharmacother., 2023, 159, 114249.
[http://dx.doi.org/10.1016/j.biopha.2023.114249] [PMID: 36682244]
[http://dx.doi.org/10.1016/j.biopha.2023.114249] [PMID: 36682244]
[74]
Wang, Y.J.; Li, Y.X.; Li, S.; He, W.; Wang, Z.R.; Zhan, T.P.; Lv, C.Y.; Liu, Y.P.; Yang, Y.; Zeng, X.X. Progress in traditional Chinese medicine and natural extracts for the treatment of lupus nephritis. Biomed. Pharmacother., 2022, 149, 112799.
[http://dx.doi.org/10.1016/j.biopha.2022.112799] [PMID: 35279011]
[http://dx.doi.org/10.1016/j.biopha.2022.112799] [PMID: 35279011]
[75]
Uyeki, T.M.; Hui, D.S.; Zambon, M.; Wentworth, D.E.; Monto, A.S. Influenza. Lancet, 2022, 400(10353), 693-706.
[http://dx.doi.org/10.1016/S0140-6736(22)00982-5] [PMID: 36030813]
[http://dx.doi.org/10.1016/S0140-6736(22)00982-5] [PMID: 36030813]
[76]
Nie, L.X.; Zha, Y.F.; Yu, J.D.; Kang, S.; Dai, Z.; Ma, S.C.; Chan, K. Quality grade evaluation of niuhuang qingwei pills based on UPLC and TCM reference drug-A novel principle of analysis of multiple components in ready-made Chinese herbal medicine. Processes, 2022, 10(6), 1166.
[http://dx.doi.org/10.3390/pr10061166]
[http://dx.doi.org/10.3390/pr10061166]
