Study of MicroRNA-124 in Patients with Lupus Nephritis

Title:Study of MicroRNA-124 in Patients with Lupus Nephritis

Volume: 24 Issue: 10

Author(s): Mostafa Abdelsalam*, Maysaa El Sayed Zaki, Nermin Youssef Abo El-Kheir, Mona Foda Salama and Asmaa Osama Bakr Seddik Osman

Affiliation:

• Department of Internal Medicine, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt and Nephrology and Dialysis Unit, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt

Abstract:

Background: Lupus nephritis is associated with a six-fold increase in mortality compared with the general population. MicroRNAs studies revealed that increased MicroRNA -21 and MicroRNA -155 levels represent risk factors for active LN patients. MicroRNAs can be used as biomarkers in the diagnosis of clinical stages of LN.

Objectives: The present study aimed to determine the level of miR-124 in patients with lupus nephritis by reverse transcriptase real-time polymerase chain reaction compared to healthy control and correlate its levels with biochemical findings in those patients.

Methods: The study was a case-control study that included fifty patients with lupus nephritis in addition to fifty healthy controls. Blood samples from the participants were subjected to the determination of serological markers of SLE. Moreover, real-time PCR was used for the determination of miR-124.

Results: The comparison of Micro-RNA124 between patients and control subjects revealed a statistically significant decrease in Micro-RNA124 in patients (1.193 ± 0.56) compared to the control (3.36 ± 0.50, p <0.001); the comparison of the level of MicroRNA 124 in the patients with different clinical and serological findings of SLE revealed a significant decrease in the level of MicroRNA 124 in patients with muscular findings (1.02 ± 0.5) compared to the patients with negative manifestations (1.47 ± 0.5, p =0.005).

Conclusion: In the present study, a comparison of MicroRNA-124 in LN patients with different stages compared to normal control showed a statistically significant decrease in Micro-RNA124 in patients with lupus nephritis p <0.001 with significant correlation to the patients’ different clinical and serological findings of SLE. Therefore, it may be used as a new noninvasive therapeutic approach to monitor response to therapy, predict relapses, and identify the degree of the activity of the disease or the progression to the chronic stage.

Cite this article as:

Abdelsalam Mostafa*, Zaki El Sayed Maysaa, Abo El-Kheir Youssef Nermin, Foda Salama Mona and Osman Osama Bakr Seddik Asmaa, Study of MicroRNA-124 in Patients with Lupus Nephritis, Endocrine, Metabolic & Immune Disorders - Drug Targets 2024; 24 (10) . https://dx.doi.org/10.2174/0118715303250919231010073608