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Current Nutrition & Food Science

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ISSN (Online): 2212-3881

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Research Article

Isolation, Molecular Characterization, and Determination of Antibiotic Resistance Patterns of Lactic Acid Bacteria from Regional Yogurt Samples and their Probiotic Properties

Author(s): Nazneen Nahar, Shahin Mahmud, Md. Shaid Bin Islam, Safaiatul Islam, Ashraf Hossain Talukder, Kaisar Ali Talukder and A. K. M. Mohiuddin*

Volume 20, Issue 6, 2024

Published on: 23 November, 2023

Page: [767 - 775] Pages: 9

DOI: 10.2174/0115734013244383230919071315

Price: $65

Abstract

Objectives: This investigation aims to isolate the Lactic Acid Bacteria (LAB) from yogurt samples from the Tangail district of Bangladesh and evaluate their probiotic properties.

Methods: A rigorous study was carried out from April to August, 2019. A total of 10 samples were examined by following the standard bacteriological methods. Besides the biochemical and antibiogram tests, molecular characterization of the isolated bacteria was carried out.

Results: Ten isolates were confirmed as LAB, and they exhibited excellent probiotic properties. They were able to survive in both acidic (pH 2) and alkaline (pH 8) conditions. They also survived against phenol (0.1-0.4%) and NaCl (1-10%) after 24 h incubation. Most of the isolates harboured plasmid DNA and showed good resistance patterns against the antibiotics used. During the mating experiment, no X-conjugants were found.

Conclusion: In conclusion, the mating experiment suggested that the acquired resistant genes might be chromosome mediated, and isolates showed good probiotic properties that confer health benefits.

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