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Protein & Peptide Letters

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ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

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Research Article

First Display of Haloalkane Dehalogenase LinB on the Surface of Bacillus subtilis Spore

Author(s): Fuli Wang, Xiujie Liu, Tianyu Song, Chengxin Pei, Qibin Huang, Hui Jiang* and Hailing Xi*

Volume 30, Issue 11, 2023

Published on: 08 November, 2023

Page: [959 - 965] Pages: 7

DOI: 10.2174/0109298665238177231020044054

Price: $65

Abstract

Background: LinB, as a Haloalkane dehalogenase, has good catalytic activity for many highly toxic and recalcitrant compounds, and can realize the elimination of chemical weapons HD in a green non-toxic mode.

Objectives: In order to display Haloalkane dehalogenase LinB on the surface of Bacillus subtilis spore.

Methods: We have constituted the B. subtilis spore surface display system of halogenated alkanes dehalogenase LinB by gene recombination.

Results: Data revealed that LinB can display on spore surface successfully. The hydrolyzing HD analogue 2-chloroethyl ethylsulfide (2-CEES) activity of displayed LinB spores was 4.30±0.09 U/mL, and its specific activity was 0.78±0.03U/mg. Meanwhile, LinB spores showed a stronger stress resistance activity on 2-CEES than free LinB. This study obtained B. subtilis spores of LinB (phingobium japonicum UT26) with enzyme activity that was not reported before.

Conclusion: Spore surface display technology uses resistance spore as the carrier to guarantee LinB activity, enhances its stability, and reduces the production cost, thus expanding the range of its application.

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Graphical Abstract

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