Abstract
Aims: This study aimed to examine the effect of magnetite coating of salicylic acid and Cu metal nanoparticles on yield, cellular contents, and some biochemical constituents of wheat subjected to heat stress.
Background: An applied experiment was conducted over two seasons at the Agricultural Experimental Station of Desert Research Center (DRC), which was supervised by the El Wadi El Gadeed Governorate in Egypt. The grains of wheat cultivars Sids1 (tolerant) and Gimmeza7 (sensitive) were treated with copper metal as NPs (Cu NPs) (0.25, 0.50, 0.75, 1.0, and 10 ppm) and magnetite NPs (0.25, 0.50, 0.75, 1.0 and 10 ppm) coated with salicylic acid at 100ppm (Fe NPs+SA).
Objective: The objective of this study was to examine wheat tolerance to heat stress and subsequently yield by comparing two wheat cultivars under the same conditions.
Methods: The chemically formulated nanoparticles were well characterized and applied in two wheat cultivars subjected to heat stress.
Results: The results showed that all NPs treatments had a positive impact on all physiological parameters and grain yield. Sids1 surpassed Gemmeiza7 in the quality of wheat grains (essential, nonessential amino acids). However, Gimmeza7 exceeded Sids1 in yield quantity, especially with the application of SA+Fe NPs at 0.50 ppm. These effects were associated with heat tolerance and the best survival in wheat cultivars. There was an increase in glutathione content, antioxidant enzymes (Glutathione -S- Transferase), and/or a decline in malondialdehyde content.
Conclusion: Fe NPs+SA (0.5ppm) helped the Gimmeza7 cultivar to mitigate the effects of heat stress through activating growth, glutathione, and glutathione S transferase, enhancing yield quantity in two wheat cultivars (Misr1 and Gimmeza11), and decreasing their MDA content.
[1]
Abdel-Azeem AA, Nada A, O’Donovan A, Kumar Thakur V, Elkelish A. Mycogenic silver nanoparticles from endophytic trichoderma atroviride with antimicrobial activity. J Renew Mater 2019; 7: 171-85.
[2]
Sangeetha J, Thangadurai D, Karekalammanavar G, et al. Nanotechnology An Agricultural Paradigm.In: Nanotechnology An Agricultural Paradigm. Singapore: Springer Nature Pte Ltd 2017; pp. 22-58.
[3]
Ditta A. How helpful is nanotechnology in agriculture? Adv Nat Sci: Nanosci Nanotechnol 2012; 3(3): 033002.
[http://dx.doi.org/10.1088/2043-6262/3/3/033002]
[http://dx.doi.org/10.1088/2043-6262/3/3/033002]
[4]
Kumar C, Igbaria A, D’Autreaux B, et al. Glutathione revisited: A vital function in iron metabolism and ancillary role in thiol-redox control. EMBO J 2011; 30(10): 2044-56.
[http://dx.doi.org/10.1038/emboj.2011.105] [PMID: 21478822]
[http://dx.doi.org/10.1038/emboj.2011.105] [PMID: 21478822]
[5]
Khan NA, Khan S, Naz N, et al. Effect of heat stress on growth, physiological and biochemical activities of wheat (Triticum aestivum L.). Int J Biosci 2017; 11(4): 173-83.
[http://dx.doi.org/10.12692/ijb/11.4.173-183]
[http://dx.doi.org/10.12692/ijb/11.4.173-183]
[6]
Hafeez A, Razaaq A, Mahmood T, Jhanzab MH. Potential of copper nanoparticles to increase growth and yield of wheat. JNAT 2015; 1(1): 6-11.
[7]
Yasmeen F, Razzaq A, Iqbal MN, Jhanzab HM. Effect of silver, copper and iron nanoparticles on wheat germination. Int J Biosci 2015; 6(4): 112-7.
[http://dx.doi.org/10.12692/ijb/6.4.112-117]
[http://dx.doi.org/10.12692/ijb/6.4.112-117]
[8]
Mahdi AA, El-Saber MM, Osman A, Hassan AH, Farroh KY. Toxicological and significant execute of biocompatible CuO nanoparticles and their influences on biochemical and molecular markers of wheat varieties under saline conditions. IOSR BB 2020; 6(5): 58-74.
[9]
Hassan NS, Salah El Din TA, Hendawey MH, Borai IH, Mahdi A. A Magnetite and zinc oxide nanoparticles alleviated heat stress in wheat plants. Curr Nanosci 2018; 3(1): 32-43.
[10]
El-Saber MM, Mahdi AA, Hassan AH, Farroh KY, Osman A. Effects of magnetite nanoparticles on physiological processes to alleviate salinity induced oxidative damage in wheat. J Sci Food Agric 2021; 101(13): 5550-62.
[http://dx.doi.org/10.1002/jsfa.11206] [PMID: 33709391]
[http://dx.doi.org/10.1002/jsfa.11206] [PMID: 33709391]
[11]
Mahdi AA. Biochemical changes in heat stressed wheat grown at El Wadi El Gedeed. MSc, thesis, faculty of science. Ain Shams University: Cairo, Egypt 2011.
[12]
Dung Dang TM, Tuyet Le TT, Fribourg-Blanc E, Chien Dang M. The influence of solvents and surfactants on the preparation of copper nanoparticles by a chemical reduction method. Advances in Natural Sciences: Nanosci Nanotechnol 2011; 2(2): 025004.
[http://dx.doi.org/10.1088/2043-6262/2/2/025004]
[http://dx.doi.org/10.1088/2043-6262/2/2/025004]
[13]
Nghiem TH, La TH, Vu XH, et al. Synthesis, capping and binding of colloidal gold nanoparticles to proteins. Adv Nat Sci Nanosci Nanotechnol 2010; 1: 1-6.
[14]
Xuan S, Hao L, Jiang W, Gong X, Hu Y, Chen Z. Preparation of water-soluble magnetite nanocrystals through hydrothermal approach. J Magn Magn Mater 2007; 308(2): 210-3.
[http://dx.doi.org/10.1016/j.jmmm.2006.05.017]
[http://dx.doi.org/10.1016/j.jmmm.2006.05.017]
[15]
Raskin I. Role of salicylic acid in plants. Annu Rev Plant Physiol Plant Mol Biol 1992; 43(1): 439-63.
[http://dx.doi.org/10.1146/annurev.pp.43.060192.002255]
[http://dx.doi.org/10.1146/annurev.pp.43.060192.002255]
[16]
Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. Arch Biochem Biophys 1968; 125(1): 189-98.
[http://dx.doi.org/10.1016/0003-9861(68)90654-1] [PMID: 5655425]
[http://dx.doi.org/10.1016/0003-9861(68)90654-1] [PMID: 5655425]
[17]
Moron MS, Depierre JW, Mannervik B. Levels of GSH, GR and GST activities in rat lungs and liver. Biochim Biophys Acta 1979; 582: 67-78.
[http://dx.doi.org/10.1016/0304-4165(79)90289-7] [PMID: 760819]
[http://dx.doi.org/10.1016/0304-4165(79)90289-7] [PMID: 760819]
[18]
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-Transferases. J Biol Chem 1974; 249(22): 7130-9.
[http://dx.doi.org/10.1016/S0021-9258(19)42083-8] [PMID: 4436300]
[http://dx.doi.org/10.1016/S0021-9258(19)42083-8] [PMID: 4436300]
[19]
Bozzola JJ, Russell LD. Electron Microscopy, Principles and Techniques For Biologists. (2nd ed.). Burlington, US: Jones and Bartlett 1998; pp. 16-48.
[20]
Pellet PL, Young VR. Nutritional evaluation of protein foods. United Nation University: Tokyo, Japan 1980.
[21]
Hamideh G, Razmjoo J. Effect of foliar application of nano-iron oxidase, iron chelate and iron sulphate rates on yield and quality of wheat. Int J Agron Plant Prod 2013; 4(11): 2997-3003.
[22]
Costa MVJ, Sharma PK. Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa. Photosynthetica 2016; 54(1): 110-9.
[http://dx.doi.org/10.1007/s11099-015-0167-5]
[http://dx.doi.org/10.1007/s11099-015-0167-5]
[23]
Dimkpa CO, McLean JE, Latta DE, et al. CuO and ZnO nanoparticles: Phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat. J Nanopart Res 2012; 14(9): 1125.
[http://dx.doi.org/10.1007/s11051-012-1125-9]
[http://dx.doi.org/10.1007/s11051-012-1125-9]
[24]
Shi Q, Bao Z, Zhu Z, Ying Q, Qian Q. Effects of different treatments of salicylic acid on heat tolerance, chlorophyll fluorescence and antioxidant enzyme activity in seedlings of Cucumis sativa L. Plant Growth Regul 2006; 48(2): 127-35.
[http://dx.doi.org/10.1007/s10725-005-5482-6]
[http://dx.doi.org/10.1007/s10725-005-5482-6]
[25]
Agarwal S, Sairam RK, Srivastava GC, Meena RC. Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biol Plant 2005; 49(4): 541-50.
[http://dx.doi.org/10.1007/s10535-005-0048-z]
[http://dx.doi.org/10.1007/s10535-005-0048-z]
[26]
Mishra A, Choudhuri MA. Effect of salicylic acid on heavy metal-induced membrane deterioration mediated by lipoxygenase in rice. Biol Plant 1999; 42(3): 409-15.
[http://dx.doi.org/10.1023/A:1002469303670]
[http://dx.doi.org/10.1023/A:1002469303670]
[27]
Waseem M, Athar HUR, Ashraf M. Effect of salicylic acid applied through rooting medium on drought tolerance of wheat. Pak J Bot 2006; 38(4): 1127-36.
[28]
Larkindale J, Knight MR. Protection against heat stress-induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol 2002; 128(2): 682-95.
[http://dx.doi.org/10.1104/pp.010320] [PMID: 11842171]
[http://dx.doi.org/10.1104/pp.010320] [PMID: 11842171]
[29]
Arfan M, Athar HR, Ashraf M. Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? J Plant Physiol 2007; 164(6): 685-94.
[http://dx.doi.org/10.1016/j.jplph.2006.05.010] [PMID: 16884826]
[http://dx.doi.org/10.1016/j.jplph.2006.05.010] [PMID: 16884826]
[30]
Hendawey MH, Abo El Fadl RE, Salah El-Din TA. Biochemical role of some nanoparticles in the production of active constituents in Stevia rebaudiana L. Callus Life Sci 2015; 12(7): 144-56.
[31]
Garrido T, Mendoza J, Riveros R, Sáez L. Acute and chronic effect of copper on levels of reduced and oxidized glutathione and nutrient uptake of tomato plants. J Plant Nutr Soil Sci 2010; 173(6): 920-6.
[http://dx.doi.org/10.1002/jpln.200800306]
[http://dx.doi.org/10.1002/jpln.200800306]
[32]
De Vos CHR, Vonk MJ, Vooijs R, Schat H. Glutathione depletion due to copper-induced phytochelatin synthesis causes oxidative stress in Silence cucubalus. Plant Physiol 1992; 98(3): 853-8.
[http://dx.doi.org/10.1104/pp.98.3.853] [PMID: 16668756]
[http://dx.doi.org/10.1104/pp.98.3.853] [PMID: 16668756]
[33]
Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 2010; 48(12): 909-30.
[http://dx.doi.org/10.1016/j.plaphy.2010.08.016] [PMID: 20870416]
[http://dx.doi.org/10.1016/j.plaphy.2010.08.016] [PMID: 20870416]
[34]
Saito K. Regulation of sulfate transport and synthesis of sulfur-containing amino acids. Curr Opin Plant Biol 2000; 3(3): 188-95.
[http://dx.doi.org/10.1016/S1369-5266(00)00063-7] [PMID: 10837270]
[http://dx.doi.org/10.1016/S1369-5266(00)00063-7] [PMID: 10837270]
[35]
Krystofova O, Sochor J, Zitka O, et al. Effect of magnetic nanoparticles on tobacco BY-2 cell suspension culture. Int J Environ Res Public Health 2012; 10(1): 47-71.
[http://dx.doi.org/10.3390/ijerph10010047] [PMID: 23343980]
[http://dx.doi.org/10.3390/ijerph10010047] [PMID: 23343980]
[36]
Kumar V, Sharma M, Khare T, Wani S. Chapter 17 - Impact of Nanoparticles on Oxidative Stress and Responsive Antioxidative Defense in Plants. Nanomaterials in Plants, Algae, and Microorganisms Concepts and Controversies. Amsterdam: Elsevier 2018; 1: pp. 393-405.
[http://dx.doi.org/10.1016/B978-0-12-811487-2.00017-7]
[http://dx.doi.org/10.1016/B978-0-12-811487-2.00017-7]
[37]
Rico CM, Majumdar S, Duarte-Gardea M, Peralta-Videa JR, Gardea-Torresdey JL. Interaction of nanoparticles with edible plants and their possible implications in the food chain. J Agric Food Chem 2011; 59(8): 3485-98.
[http://dx.doi.org/10.1021/jf104517j] [PMID: 21405020]
[http://dx.doi.org/10.1021/jf104517j] [PMID: 21405020]
[38]
Watanabe T, Misawa S, Hiradate S, Osaki M. Root mucilage enhances aluminum accumulation in Melastoma malabathricum, an aluminum accumulator. Plant Signal Behav 2008; 3(8): 603-5.
[http://dx.doi.org/10.4161/psb.3.8.6356] [PMID: 19704812]
[http://dx.doi.org/10.4161/psb.3.8.6356] [PMID: 19704812]
[39]
Mehrian SK, Heidari R, Rahmani F. Effect of silver nanoparticles on free amino acids content and antioxidant defense system of tomato plants. Ind J Pl. Physiol 2015; 20(3): 257-63.
[40]
Nair PMG, Chung IM, Chung IM. Impact of copper oxide nanoparticles exposure on Arabidopsis thaliana growth, root system development, root lignificaion, and molecular level changes. Environ Sci Pollut Res Int 2014; 21(22): 12709-22.
[http://dx.doi.org/10.1007/s11356-014-3210-3] [PMID: 24965006]
[http://dx.doi.org/10.1007/s11356-014-3210-3] [PMID: 24965006]
[41]
Yuan M, Guo X, Pang H. Derivatives (Cu/CuO, Cu/Cu2O, and CuS) of Cu superstructures reduced by biomass reductants. Mater Today Chem 2021; 21: 100519.
[http://dx.doi.org/10.1016/j.mtchem.2021.100519]
[http://dx.doi.org/10.1016/j.mtchem.2021.100519]
[42]
Bai Y, Liu C, Chen T, et al. MXene-copper/cobalt hybrids via lewis acidic molten salts etching for high performance symmetric supercapacitors. Angew Chem 2021; 133(48): 25522-6.
[http://dx.doi.org/10.1002/ange.202112381]
[http://dx.doi.org/10.1002/ange.202112381]
[43]
Abou El-Nasr M. A Model For Using Iron Nanoparticales As A Nutritive Support For Some Fruit Trees Transplants. MSc Thesis, Faculty of Agriculture Ain Shams University: Cairo, Egypt 2015.
[44]
Fouda MS, Hendawey MH, Hegazi GA. Sharada HM, El-Arabi NI, Attia ME, Soliman ERS Nanoparticles induce genetic, biochemical, and ultrastructure variations in Salvadora persica callus. J Genet Eng Biotechnol 2021; 19(1): 27.
[http://dx.doi.org/10.1186/s43141-021-00124-3]
[http://dx.doi.org/10.1186/s43141-021-00124-3]
