Recent Approaches to Enhance Osteogenesis of Dental Pulp Stem Cells on
Electrospun Scaffolds

Zahra      Safari; Seyedeh Sara      Aghili; Sahar      Hassantash; Ehsan      Iranmanesh; Mehdi      Abouali; Mobina      Bagherianlemraski; Shabnam      Ghasemzadeh; Esmaeel      Dadgar; Ghasem      Barati; Ehsan      Saburi
doi:10.2174/1574888X18666230530153521
Abstract

Critical-sized bone defects are a challenging issue during bone regeneration. Bone tissue engineering is aimed to repair such defects using biomimicking scaffolds and stem cells. Electrospinning allows the fabrication of biocompatible, biodegradable, and strengthened scaffolds for bone regeneration. Natural and synthetic polymers, alone or in combination, have been employed to fabricate scaffolds with appropriate properties for the osteogenic differentiation of stem cells. Dental pulps are rich in stem cells, and dental pulp stem cells (DPSCs) have a high capacity for proliferation, differentiation, immunomodulation, and trophic factor expression. Researchers have tried to enhance osteogenesis through scaffold modification approaches, including incorporation or coating with mineral, inorganic materials, and herbal extract components. Among them, the incorporation of nanofibers with hyaluronic acid (HA) has been widely used to promote osteogenesis. In this review, the electrospun scaffolds and their modifications used in combination with DPSCs for bone regeneration are discussed.
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Graphical Abstract

[1]
Perry CR. Bone repair techniques, bone graft, and bone graft substitutes. Clin Orthop Relat Res  1999; 360(360): 71-86.
 [http://dx.doi.org/10.1097/00003086-199903000-00010] [PMID: 10101312]

[2]
Wani TU, Khan RS, Rather AH, Abdal-hay A, Amna T, Sheikh FA. Nanofiber-mediated stem cell osteogenesis: Prospects in bone tissue regeneration. In: Sheikh FA, Ed. Engineering Materials for Stem Cell Regeneration.  Springer Singapore, Singapore 2021; pp. 47-67.
 [http://dx.doi.org/10.1007/978-981-16-4420-7_3]

[3]
Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol  2003; 63(15): 2223-53.
 [http://dx.doi.org/10.1016/S0266-3538(03)00178-7]

[4]
Brown C, McKee C, Bakshi S, et al. Mesenchymal stem cells: Cell therapy and regeneration potential. J Tissue Eng Regen Med  2019; 13(9): 1738-55.
 [http://dx.doi.org/10.1002/term.2914] [PMID: 31216380]

[5]
Bar JK, Kowalczyk T, Grelewski PG, et al. Characterization of biological properties of dental pulp stem cells grown on an electrospun poly(l-lactide-co-caprolactone) scaffold. Materials (Basel)  2022; 15(5): 1900.
 [http://dx.doi.org/10.3390/ma15051900] [PMID: 35269131]

[6]
Bar JK, Lis-Nawara A, Grelewski PG. Dental pulp stem cell-derived secretome and its regenerative potential. Int J Mol Sci  2021; 22(21): 12018.
 [http://dx.doi.org/10.3390/ijms222112018] [PMID: 34769446]

[7]
Vazin T, Freed WJ. Human embryonic stem cells: Derivation, culture, and differentiation: A review. Restor Neurol Neurosci  2010; 28(4): 589-603.
 [http://dx.doi.org/10.3233/RNN-2010-0543] [PMID: 20714081]

[8]
Leeb C, Jurga M, McGuckin C, et al. New perspectives in stem cell research: Beyond embryonic stem cells. Cell Prolif  2011; 44(S1): 9-14.
 [http://dx.doi.org/10.1111/j.1365-2184.2010.00725.x] [PMID: 21481037]

[9]
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. cell  2006; 126(4): 663-76.

[10]
Fibbe WE, Nauta AJ, Roelofs H. Modulation of immune responses by mesenchymal stem cells. Ann N Y Acad Sci  2007; 1106(1): 272-8.
 [http://dx.doi.org/10.1196/annals.1392.025] [PMID: 17442776]

[11]
Anitua E, Troya M, Zalduendo M. Progress in the use of dental pulp stem cells in regenerative medicine. Cytotherapy  2018; 20(4): 479-98.
 [http://dx.doi.org/10.1016/j.jcyt.2017.12.011] [PMID: 29449086]

[12]
Galipeau J, Krampera M, Barrett J, et al. International Society for Cellular Therapy perspective on immune functional assays for mesenchymal stromal cells as potency release criterion for advanced phase clinical trials. Cytotherapy  2016; 18(2): 151-9.
 [http://dx.doi.org/10.1016/j.jcyt.2015.11.008] [PMID: 26724220]

[13]
Gugliandolo A, Fonticoli L, Trubiani O, et al. Oral bone tissue regeneration: mesenchymal stem cells, secretome, and biomaterials. Int J Mol Sci  2021; 22(10): 5236.
 [http://dx.doi.org/10.3390/ijms22105236] [PMID: 34063438]

[14]
L PK, Kandoi S, Misra R, S V, K R, Verma RS. The mesenchymal stem cell secretome: A new paradigm towards cell-free therapeutic mode in regenerative medicine. Cytokine Growth Factor Rev  2019; 46: 1-9.
 [http://dx.doi.org/10.1016/j.cytogfr.2019.04.002] [PMID: 30954374]

[15]
Morsczeck C, Reichert TE. Dental stem cells in tooth regeneration and repair in the future. Expert Opin Biol Ther  2018; 18(2): 187-96.
 [http://dx.doi.org/10.1080/14712598.2018.1402004] [PMID: 29110535]

[16]
Morad G, Kheiri L, Khojasteh A. Dental pulp stem cells for in vivo bone regeneration: A systematic review of literature. Arch Oral Biol  2013; 58(12): 1818-27.
 [http://dx.doi.org/10.1016/j.archoralbio.2013.08.011] [PMID: 24095289]

[17]
Rahmati M, Mills DK, Urbanska AM, et al. Electrospinning for tissue engineering applications. Prog Mater Sci  2021; 117: 100721.
 [http://dx.doi.org/10.1016/j.pmatsci.2020.100721]

[18]
Azari Matin A, Fattah K, Saeidpour MS, et al. Synthetic electrospun nanofibers as a supportive matrix in osteogenic differentiation of induced pluripotent stem cells. J Biomater Sci Polym Ed  2022; 33(11): 1469-93.
 [http://dx.doi.org/10.1080/09205063.2022.2056941] [PMID: 35321624]

[19]
Barnes CP, Sell SA, Boland ED, Simpson DG, Bowlin GL. Nanofiber technology: Designing the next generation of tissue engineering scaffolds. Adv Drug Deliv Rev  2007; 59(14): 1413-33.
 [http://dx.doi.org/10.1016/j.addr.2007.04.022] [PMID: 17916396]

[20]
Liu X, Ma PX. Phase separation, pore structure, and properties of nanofibrous gelatin scaffolds. Biomaterials  2009; 30(25): 4094-103.
 [http://dx.doi.org/10.1016/j.biomaterials.2009.04.024] [PMID: 19481080]

[21]
Prasad A, Sankar MR, Katiyar V. State of art on solvent casting particulate leaching method for orthopedic scaffoldsfabrication. Mater Today Proc  2017; 4(2): 898-907.
 [http://dx.doi.org/10.1016/j.matpr.2017.01.101]

[22]
Chahal S, Kumar A, Hussian FSJ. Development of biomimetic electrospun polymeric biomaterials for bone tissue engineering. A review. J Biomater Sci Polym Ed  2019; 30(14): 1308-55.
 [http://dx.doi.org/10.1080/09205063.2019.1630699] [PMID: 31181982]

[23]
Asghari F, Samiei M, Adibkia K, Akbarzadeh A, Davaran S. Biodegradable and biocompatible polymers for tissue engineering application: A review. Artif Cells Nanomed Biotechnol  2017; 45(2): 185-92.
 [http://dx.doi.org/10.3109/21691401.2016.1146731] [PMID: 26923861]

[24]
Koons GL, Diba M, Mikos AG. Materials design for bone-tissue engineering. Nat Rev Mater  2020; 5(8): 584-603.
 [http://dx.doi.org/10.1038/s41578-020-0204-2]

[25]
Hashemi J, Barati G, Enderami SE, Safdari M. Osteogenic differentiation of induced pluripotent stem cells on electrospun nanofibers: A review of literature. Mater Commun  2020; 25: 101561.

[26]
Su WT, Wu PS, Huang TY. Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly(ε-caprolactone) nanofibers containing strontium phosphate. Mater Sci Eng C  2015; 46: 427-34.
 [http://dx.doi.org/10.1016/j.msec.2014.10.076] [PMID: 25492007]

[27]
Samanipour R, Farzaneh S, Ranjbari J, Hashemi S, Khojasteh A, Hosseinzadeh S. Osteogenic differentiation of pulp stem cells from human permanent teeth on an oxygen-releasing electrospun scaffold. Polym Bull  2022; 80: 1795-816.

[28]
Aghazadeh M, Samiei M, Alizadeh E, Porkar P, Bakhtiyari M, Salehi R. Towards osteogenic bioengineering of dental pulp stem induced by sodium fluoride on hydroxyapatite based biodegradable polymeric scaffold. Fibers Polym  2017; 18(8): 1468-77.
 [http://dx.doi.org/10.1007/s12221-017-7120-0]

[29]
Oliveira NK, Salles THC, Pedroni AC, et al. Osteogenic potential of human dental pulp stem cells cultured onto poly-ε-caprolactone/poly (rotaxane) scaffolds. Dent Mater  2019; 35(12): 1740-9.
 [http://dx.doi.org/10.1016/j.dental.2019.08.109] [PMID: 31543375]

[30]
Ma L, Yu Y, Liu H, et al. Berberine-releasing electrospun scaffold induces osteogenic differentiation of DPSCs and accelerates bone repair. Sci Rep  2021; 11(1): 1027.
 [http://dx.doi.org/10.1038/s41598-020-79734-9] [PMID: 33441759]

[31]
Alipour M, Aghazadeh M, Akbarzadeh A, Vafajoo Z, Aghazadeh Z, Raeisdasteh HV. Towards osteogenic differentiation of human dental pulp stem cells on PCL-PEG-PCL/zeolite nanofibrous scaffolds. Artif Cells Nanomed Biotechnol  2019; 47(1): 3431-7.
 [http://dx.doi.org/10.1080/21691401.2019.1652627] [PMID: 31411067]

[32]
Hosseini FS, Enderami SE, Hadian A, et al. Efficient osteogenic differentiation of the dental pulp stem cells on β‐glycerophosphate loaded polycaprolactone/polyethylene oxide blend nanofibers. J Cell Physiol  2019; 234(8): 13951-8.
 [http://dx.doi.org/10.1002/jcp.28078] [PMID: 30633333]

[33]
Akkouch A, Zhang Z, Rouabhia M. Engineering bone tissue using human dental pulp stem cells and an osteogenic collagen-hydroxyapatite-poly (l -lactide-co-ɛ-caprolactone) scaffold. J Biomater Appl  2014; 28(6): 922-36.
 [http://dx.doi.org/10.1177/0885328213486705] [PMID: 23640860]

[34]
Ko EK, Jeong SI, Rim NG, Lee YM, Shin H, Lee BK. In vitro osteogenic differentiation of human mesenchymal stem cells and in vivo bone formation in composite nanofiber meshes. Tissue Eng Part A  2008; 14(12): 2105-19.
 [http://dx.doi.org/10.1089/ten.tea.2008.0057] [PMID: 18788980]

[35]
Sanaei-rad P, Jamshidi D, Adel M, Seyedjafari E. Electrospun poly(l ‐lactide) nanofibers coated with mineral trioxide aggregate enhance odontogenic differentiation of dental pulp stem cells. Polym Adv Technol  2021; 32(1): 402-10.
 [http://dx.doi.org/10.1002/pat.5095]

[36]
Gonçalves F, Bentini R, Burrows M, et al. Hybrid membranes of PLLA/Collagen for bone tissue engineering: A comparative study of scaffold production techniques for optimal mechanical properties and osteoinduction ability. Materials  2015; 8(2): 408-23.
 [http://dx.doi.org/10.3390/ma8020408] [PMID: 28787946]

[37]
Asghari F, Salehi R, Agazadeh M, et al. The odontogenic differentiation of human dental pulp stem cells on hydroxyapatite-coated biodegradable nanofibrous scaffolds. Int J Polym Mater  2016; 65(14): 720-8.
 [http://dx.doi.org/10.1080/00914037.2016.1163564]

[38]
Sohrabi A, Hosseini M, Abazari MF, et al. Wnt pathway activator delivery by poly (lactide-co-glycolide)/silk fibroin composite nanofibers promotes dental pulp stem cell osteogenesis. J Drug Deliv Sci Technol  2021; 61: 102223.
 [http://dx.doi.org/10.1016/j.jddst.2020.102223]

[39]
Zhang L, Feng KC, Yu Y, et al. Effect of graphene on differentiation and mineralization of dental pulp stem cells in poly(4-vinylpyridine) matrix in vitro. ACS Appl Bio Mater  2019; 2(6): 2435-43.
 [http://dx.doi.org/10.1021/acsabm.9b00127] [PMID: 35030700]

[40]
Dwivedi R, Kumar S, Pandey R, et al. Polycaprolactone as biomaterial for bone scaffolds: Review of literature. J Oral Biol Craniofac Res  2020; 10(1): 381-8.
 [http://dx.doi.org/10.1016/j.jobcr.2019.10.003] [PMID: 31754598]

[41]
Engelberg I, Kohn J. Physico-mechanical properties of degradable polymers used in medical applications: A comparative study. Biomaterials  1991; 12(3): 292-304.
 [http://dx.doi.org/10.1016/0142-9612(91)90037-B] [PMID: 1649646]

[42]
Anderson JM, Shive MS. Biodegradation and biocompatibility of PLA and PLGA microspheres. Adv Drug Deliv Rev  2012; 64: 72-82.
 [http://dx.doi.org/10.1016/j.addr.2012.09.004]

[43]
Kang R, Luo Y, Zou L, et al. Osteogenesis of human induced pluripotent stem cells derived mesenchymal stem cells on hydroxyapatite contained nanofibers. RSC Advances  2014; 4(11): 5734-9.
 [http://dx.doi.org/10.1039/c3ra44181d]

[44]
Ardeshirylajimi A, Khojasteh A. Synergism of electrospun nanofibers and pulsed electromagnetic field on osteogenic differentiation of induced pluripotent stem cells. ASAIO J  2018; 64(2): 253-60.
 [http://dx.doi.org/10.1097/MAT.0000000000000631] [PMID: 28746081]

[45]
Soleimanifar F, Hosseini FS, Atabati H, et al. Adipose‐derived stem cells‐conditioned medium improved osteogenic differentiation of induced pluripotent stem cells when grown on polycaprolactone nanofibers. J Cell Physiol  2019; 234(7): 10315-23.
 [http://dx.doi.org/10.1002/jcp.27697] [PMID: 30378123]

[46]
Deitzel J, Kleinmeyer JD, Hirvonen JK, Beck Tan NC. Controlled deposition of electrospun poly(ethylene oxide) fibers. Polymer  2001; 42(19): 8163-70.
 [http://dx.doi.org/10.1016/S0032-3861(01)00336-6]

[47]
Gaaz T, Sulong A, Akhtar M, Kadhum A, Mohamad A, Al-Amiery A. Properties and applications of polyvinyl alcohol, halloysite nanotubes and their nanocomposites. Molecules  2015; 20(12): 22833-47.
 [http://dx.doi.org/10.3390/molecules201219884] [PMID: 26703542]

[48]
Azadian E, Arjmand B, Ardeshirylajimi A, Hosseinzadeh S, Omidi M, Khojasteh A. Polyvinyl alcohol modified polyvinylidene fluoride‐graphene oxide scaffold promotes osteogenic differentiation potential of human induced pluripotent stem cells. J Cell Biochem  2020; 121(5-6): 3185-96.
 [http://dx.doi.org/10.1002/jcb.29585] [PMID: 31886565]

[49]
Kashef-Saberi MS, Hayati Roodbari N, Parivar K, Vakilian S, Hanaee-Ahvaz H. Enhanced osteogenic differentiation of mesenchymal stem cells on electrospun polyethersulfone/Poly(Vinyl) alcohol/platelet rich plasma nanofibrous scaffolds. ASAIO J  2018; 64(5): e115-22.
 [http://dx.doi.org/10.1097/MAT.0000000000000781] [PMID: 30142100]

[50]
Aslam M, Kalyar MA, Raza ZA. Polyvinyl alcohol: A review of research status and use of polyvinyl alcohol based nanocomposites. Polym Eng Sci  2018; 58(12): 2119-32.
 [http://dx.doi.org/10.1002/pen.24855]

[51]
Feldman D. Poly(Vinyl Alcohol) recent contributions to engineering and medicine. Journal of Composites Science  2020; 4(4): 175.
 [http://dx.doi.org/10.3390/jcs4040175]

[52]
Jundziłł A, Pokrywczyńska M, Adamowicz J, et al. Vascularization potential of electrospun poly(L-Lactide-co-Caprolactone) Scaffold: The impact for tissue engineering. Med Sci Monit  2017; 23: 1540-51.
 [http://dx.doi.org/10.12659/MSM.899659] [PMID: 28360409]

[53]
Kloskowski T. Jundziłł A, Kowalczyk T, et al. Ureter regeneration-the proper scaffold has to be defined. PLoS One  2014; 9(8): e106023.
 [http://dx.doi.org/10.1371/journal.pone.0106023] [PMID: 25162415]

[54]
Simamora P, Chern W. Poly-L-lactic acid: An overview. J Drugs Dermatol  2006; 5(5): 436-40.
 [PMID: 16703779]

[55]
Eling B, Gogolewski S, Pennings AJ. Biodegradable materials of poly(l-lactic acid): 1. Melt-spun and solution-spun fibres. Polymer  1982; 23(11): 1587-93.
 [http://dx.doi.org/10.1016/0032-3861(82)90176-8]

[56]
Walton M, Cotton NJ. Long-term in vivo degradation of poly-L-lactide (PLLA) in bone. J Biomater Appl  2007; 21(4): 395-411.
 [http://dx.doi.org/10.1177/0885328206065125] [PMID: 16684797]

[57]
D’Angelo F, Armentano I, Cacciotti I, et al. Tuning multi/pluri-potent stem cell fate by electrospun poly(L-lactic acid)-calcium-deficient hydroxyapatite nanocomposite mats. Biomacromolecules  2012; 13(5): 1350-60.
 [http://dx.doi.org/10.1021/bm3000716] [PMID: 22449037]

[58]
Izadpanahi M, Seyedjafari E, Arefian E, et al. Nanotopographical cues of electrospun PLLA efficiently modulate non-coding RNA network to osteogenic differentiation of mesenchymal stem cells during BMP signaling pathway. Mater Sci Eng C  2018; 93: 686-703.
 [http://dx.doi.org/10.1016/j.msec.2018.08.023] [PMID: 30274102]

[59]
Karimi Z, Seyedjafari E, Mahdavi FS, et al. Baghdadite nanoparticle‐coated poly l ‐lactic acid (PLLA) ceramics scaffold improved osteogenic differentiation of adipose tissue‐derived mesenchymal stem cells. J Biomed Mater Res A  2019; 107(6): 1284-93.
 [http://dx.doi.org/10.1002/jbm.a.36638] [PMID: 30706628]

[60]
Hu M, Deng C, Gu X, Fu Q, Zhang J. Manipulating the strength–toughness balance of poly(l -lactide) (PLLA) via introducing ductile poly(ε-caprolactone) (PCL) and strong shear flow. Ind Eng Chem Res  2020; 59(2): 1000-9.
 [http://dx.doi.org/10.1021/acs.iecr.9b05380]

[61]
Lin CC, Fu SJ. Osteogenesis of human adipose-derived stem cells on poly(dopamine)-coated electrospun poly(lactic acid) fiber mats. Mater Sci Eng C  2016; 58: 254-63.
 [http://dx.doi.org/10.1016/j.msec.2015.08.009] [PMID: 26478309]

[62]
Helal MH, Hendawy HD, Gaber RA, Helal NR, Aboushelib MN. Osteogenesis ability of CAD-CAM biodegradable polylactic acid scaffolds for reconstruction of jaw defects. J Prosthet Dent  2019; 121(1): 118-23.
 [http://dx.doi.org/10.1016/j.prosdent.2018.03.033] [PMID: 29961633]

[63]
Jin S, Xia X, Huang J, et al. Recent advances in PLGA-based biomaterials for bone tissue regeneration. Acta Biomater  2021; 127: 56-79.
 [http://dx.doi.org/10.1016/j.actbio.2021.03.067] [PMID: 33831569]

[64]
Gentile P, Chiono V, Carmagnola I, Hatton P. An overview of poly(lactic-co-glycolic) acid (PLGA)-based biomaterials for bone tissue engineering. Int J Mol Sci  2014; 15(3): 3640-59.
 [http://dx.doi.org/10.3390/ijms15033640] [PMID: 24590126]

[65]
Zhang L, Yu Y, Feng K, et al. Templated dentin formation by dental pulp stem cells on banded collagen bundles nucleated on electrospun poly (4-vinyl pyridine) fibers in vitro. Acta Biomater  2018; 76: 80-8.
 [http://dx.doi.org/10.1016/j.actbio.2018.06.028] [PMID: 29940368]

[66]
Venkatesan J, Kim SK. Nano-hydroxyapatite composite biomaterials for bone tissue engineering--a review. J Biomed Nanotechnol  2014; 10(10): 3124-40.
 [http://dx.doi.org/10.1166/jbn.2014.1893] [PMID: 25992432]

[67]
Qin J, Yang D, Maher S, et al. Micro- and nano-structured 3D printed titanium implants with a hydroxyapatite coating for improved osseointegration. J Mater Chem B Mater Biol Med  2018; 6(19): 3136-44.
 [http://dx.doi.org/10.1039/C7TB03251J] [PMID: 32254348]

[68]
Pepla E, Besharat LK, Palaia G, Tenore G, Migliau G. Nano-hydroxyapatite and its applications in preventive, restorative and regenerative dentistry: A review of literature. Ann Stomatol  2014; 5(3): 108-14.
 [http://dx.doi.org/10.11138/ads/2014.5.3.108] [PMID: 25506416]

[69]
Xu R, Zhang Z, Toftdal MS, et al. Synchronous delivery of hydroxyapatite and connective tissue growth factor derived osteoinductive peptide enhanced osteogenesis. J Control Release  2019; 301: 129-39.
 [http://dx.doi.org/10.1016/j.jconrel.2019.02.037] [PMID: 30880079]

[70]
Fang R, Zhang E, Xu L, Wei S. Electrospun PCL/PLA/HA based nanofibers as scaffold for osteoblast-like cells. J Nanosci Nanotechnol  2010; 10(11): 7747-51.
 [http://dx.doi.org/10.1166/jnn.2010.2831] [PMID: 21138024]

[71]
Coelho MJ, Fernandes MH. Human bone cell cultures in biocompatibility testing. Part II: effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation. Biomaterials  2000; 21(11): 1095-102.
 [http://dx.doi.org/10.1016/S0142-9612(99)00192-1] [PMID: 10817261]

[72]
Erisken C, Kalyon DM, Wang H, Örnek-Ballanco C, Xu J. Osteochondral tissue formation through adipose-derived stromal cell differentiation on biomimetic polycaprolactone nanofibrous scaffolds with graded insulin and Beta-glycerophosphate concentrations. Tissue Eng Part A  2011; 17(9-10): 1239-52.
 [http://dx.doi.org/10.1089/ten.tea.2009.0693] [PMID: 21189068]

[73]
Krawetz RJ, Taiani JT, Wu YE, et al. Collagen I scaffolds cross-linked with beta-glycerol phosphate induce osteogenic differentiation of embryonic stem cells in vitro and regulate their tumorigenic potential in vivo. Tissue Eng Part A  2012; 18(9-10): 1014-24.
 [http://dx.doi.org/10.1089/ten.tea.2011.0174] [PMID: 22166057]

[74]
Hassanian SM, Ardeshirylajimi A, Dinarvand P, Rezaie AR. Inorganic polyphosphate promotes cyclin D1 synthesis through activation of mTOR/Wnt/β‐catenin signaling in endothelial cells. J Thromb Haemost  2016; 14(11): 2261-73.
 [http://dx.doi.org/10.1111/jth.13477] [PMID: 27546592]

[75]
Yan M, Wu J, Yu Y, et al. Mineral trioxide aggregate promotes the odonto/osteogenic differentiation and dentinogenesis of stem cells from apical papilla via nuclear factor kappa B signaling pathway. J Endod  2014; 40(5): 640-7.
 [http://dx.doi.org/10.1016/j.joen.2014.01.042] [PMID: 24767557]

[76]
Wang Y, Li J, Song W, Yu J. Mineral trioxide aggregate upregulates odonto/osteogenic capacity of bone marrow stromal cells from craniofacial bones via JNK and ERK MAPK signalling pathways. Cell Prolif  2014; 47(3): 241-8.
 [http://dx.doi.org/10.1111/cpr.12099] [PMID: 24635197]

[77]
Holt BD, Wright ZM, Arnold AM, Sydlik SA. Graphene oxide as a scaffold for bone regeneration. Wiley Interdiscip Rev Nanomed Nanobiotechnol  2017; 9(3): e1437.
 [http://dx.doi.org/10.1002/wnan.1437] [PMID: 27781398]

[78]
Xie Y, Li H, Ding C, Zheng X, Li K. Effects of graphene plates’ adoption on the microstructure, mechanical properties, and in vivo biocompatibility of calcium silicate coating. Int J Nanomedicine  2015; 10: 3855-63.
 [http://dx.doi.org/10.2147/IJN.S77919] [PMID: 26089662]

[79]
Yan Y, Zhang X, Mao H, Huang Y, Ding Q, Pang X. Hydroxyapatite/gelatin functionalized graphene oxide composite coatings deposited on TiO2 nanotube by electrochemical deposition for biomedical applications. Appl Surf Sci  2015; 329: 76-82.
 [http://dx.doi.org/10.1016/j.apsusc.2014.12.115]

[80]
Duan S, Yang X, Mei F, et al. Enhanced osteogenic differentiation of mesenchymal stem cells on poly(l -lactide) nanofibrous scaffolds containing carbon nanomaterials. J Biomed Mater Res A  2015; 103(4): 1424-35.
 [http://dx.doi.org/10.1002/jbm.a.35283] [PMID: 25046153]

[81]
Santos C, Piedade C, Uggowitzer PJ, Montemor MF, Carmezim MJ. Parallel nano-assembling of a multifunctional GO/HapNP coating on ultrahigh-purity magnesium for biodegradable implants. Appl Surf Sci  2015; 345: 387-93.
 [http://dx.doi.org/10.1016/j.apsusc.2015.03.182]

[82]
Zeng Y, Pei X, Yang S, et al. Graphene oxide/hydroxyapatite composite coatings fabricated by electrochemical deposition. Surf Coat Tech  2016; 286: 72-9.
 [http://dx.doi.org/10.1016/j.surfcoat.2015.12.013]

[83]
Wang JK, Xiong GM, Zhu M, et al. Polymer-enriched 3D graphene foams for biomedical applications. ACS Appl Mater Interfaces  2015; 7(15): 8275-83.
 [http://dx.doi.org/10.1021/acsami.5b01440] [PMID: 25822669]

[84]
Saburi E, Islami M, Hosseinzadeh S, et al. In vitro osteogenic differentiation potential of the human induced pluripotent stem cells augments when grown on Graphene oxide-modified nanofibers. Gene  2019; 696: 72-9.
 [http://dx.doi.org/10.1016/j.gene.2019.02.028] [PMID: 30772518]

[85]
Elkhenany H, Amelse L, Lafont A, et al. Graphene supports in vitro proliferation and osteogenic differentiation of goat adult mesenchymal stem cells: Potential for bone tissue engineering. J Appl Toxicol  2015; 35(4): 367-74.
 [http://dx.doi.org/10.1002/jat.3024] [PMID: 25220951]

[86]
Kumar S, Raj S, Sarkar K, Chatterjee K. Engineering a multi-biofunctional composite using poly(ethylenimine) decorated graphene oxide for bone tissue regeneration. Nanoscale  2016; 8(12): 6820-36.
 [http://dx.doi.org/10.1039/C5NR06906H] [PMID: 26955801]

[87]
Luo Y, Shen H, Fang Y, et al. Enhanced proliferation and osteogenic differentiation of mesenchymal stem cells on graphene oxide-incorporated electrospun poly(lactic-co-glycolic acid) nanofibrous mats. ACS Appl Mater Interfaces  2015; 7(11): 6331-9.
 [http://dx.doi.org/10.1021/acsami.5b00862] [PMID: 25741576]

[88]
Hoseinpour V, Shariatinia Z. Applications of zeolitic imidazolate framework-8 (ZIF-8) in bone tissue engineering: A review. Tissue Cell  2021; 72: 101588.
 [http://dx.doi.org/10.1016/j.tice.2021.101588] [PMID: 34237482]

[89]
Zarrintaj P, Mahmodi G, Manouchehri S, et al. Zeolite in tissue engineering: Opportunities and challenges. MedComm  2020; 1(1): 5-34.
 [http://dx.doi.org/10.1002/mco2.5] [PMID: 34766107]

[90]
Zhu R, Chen YX, Ke QF, Gao YS, Guo YP. SC79-loaded ZSM-5/chitosan porous scaffolds with enhanced stem cell osteogenic differentiation and bone regeneration. J Mater Chem B Mater Biol Med  2017; 5(25): 5009-18.
 [http://dx.doi.org/10.1039/C7TB00897J] [PMID: 32264018]

[91]
Gao X, Xue Y, Zhu Z, et al. Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair. ACS Appl Mater Interfaces  2021; 13(1): 97-111.
 [http://dx.doi.org/10.1021/acsami.0c15945] [PMID: 33354968]

[92]
Wang X, Qiu X, Pei J, Zhao D, Yan Y. Fabrication of magnesium phosphate bone cement with enhanced osteogenic properties by employing zeolitic imidazolate framework-8. J Mater Res  2022; 37(17): 2761-74.
 [http://dx.doi.org/10.1557/s43578-022-00663-6]

[93]
Choudhary S, Halbout P, Alander C, Raisz L, Pilbeam C. Strontium ranelate promotes osteoblastic differentiation and mineralization of murine bone marrow stromal cells: Involvement of prostaglandins. J Bone Miner Res  2007; 22(7): 1002-10.
 [http://dx.doi.org/10.1359/jbmr.070321] [PMID: 17371157]

[94]
Peng S, Liu XS, Wang T, et al. In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells. J Orthop Res  2010; 28(9): 1208-14.
 [http://dx.doi.org/10.1002/jor.21127] [PMID: 20196084]

[95]
Marie PJ. Strontium ranelate: A physiological approach for optimizing bone formation and resorption. Bone  2006; 38(2 (Supp1)): 10-4.
 [http://dx.doi.org/10.1016/j.bone.2005.07.029] [PMID: 16439191]

[96]
Ammann P. Strontium ranelate: A physiological approach for an improved bone quality. Bone  2006; 38(S2): 15-8.
 [http://dx.doi.org/10.1016/j.bone.2005.09.023] [PMID: 16455318]

[97]
Kalalinia F, Ghasim H, Amel Farzad S, Pishavar E, Ramezani M, Hashemi M. Comparison of the effect of crocin and crocetin, two major compounds extracted from saffron, on osteogenic differentiation of mesenchymal stem cells. Life Sci  2018; 208: 262-7.
 [http://dx.doi.org/10.1016/j.lfs.2018.07.043] [PMID: 30048694]

[98]
Saah S, Siriwan D, Trisonthi P. Biological activities of Boesenbergia rotunda parts and extracting solvents in promoting osteogenic differentiation of pre-osteoblasts. Food Biosci  2021; 41: 101011.
 [http://dx.doi.org/10.1016/j.fbio.2021.101011]

[99]
Natural Herb Mixture Extract Accelerates Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Activating the SMAD Pathway J Med Food  2021; 24(11): 1145-52.
 [PMID: 34792394]

[100]
Li H, Miyahara T, Tezuka Y, Tran QL, Seto H, Kadota S. Effect of berberine on bone mineral density in SAMP6 as a senile osteoporosis model. Biol Pharm Bull  2003; 26(1): 110-1.
 [http://dx.doi.org/10.1248/bpb.26.110] [PMID: 12520186]

[101]
Zhang R, Yang J, Wu J, et al. Berberine promotes osteogenic differentiation of mesenchymal stem cells with therapeutic potential in periodontal regeneration. Eur J Pharmacol  2019; 851: 144-50.
 [http://dx.doi.org/10.1016/j.ejphar.2019.02.026] [PMID: 30776366]

[102]
Liu J, Zhao X, Pei D, et al. The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR. Sci Rep  2018; 8(1): 2848.
 [http://dx.doi.org/10.1038/s41598-018-21116-3] [PMID: 29434321]

[103]
Xin BC, Wu QS, Jin S, Luo AH, Sun DG, Wang F. Berberine promotes osteogenic differentiation of human dental pulp stem cells through activating EGFR-MAPK-Runx2 pathways. Pathol Oncol Res  2020; 26(3): 1677-85.
 [http://dx.doi.org/10.1007/s12253-019-00746-6] [PMID: 31598896]

[104]
Yao Q, Cosme JGL, Xu T, et al. Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone formation. Biomaterials  2017; 115: 115-27.
 [http://dx.doi.org/10.1016/j.biomaterials.2016.11.018] [PMID: 27886552]

[105]
Abazari MF, Nejati F, Nasiri N, et al. Platelet-rich plasma incorporated electrospun PVA-chitosan-HA nanofibers accelerates osteogenic differentiation and bone reconstruction. Gene  2019; 720: 144096.
 [http://dx.doi.org/10.1016/j.gene.2019.144096] [PMID: 31476405]

[106]
Tanikawa DYS, Pinheiro CCG, Almeida MCA, et al. Deciduous dental pulp stem cells for maxillary alveolar reconstruction in cleft lip and palate patients. Stem Cells Int  2020; 2020: 6234167.

[107]
De Mori A, Peña Fernández M, Blunn G, Tozzi G, Roldo M. 3D printing and electrospinning of composite hydrogels for cartilage and bone tissue engineering. Polymers  2018; 10(3): 285.
 [http://dx.doi.org/10.3390/polym10030285] [PMID: 30966320]

[108]
Hong N, Yang GH, Lee J, Kim G. 3D bioprinting and its in vivo applications. J Biomed Mater Res B Appl Biomater  2018; 106(1): 444-59.
 [http://dx.doi.org/10.1002/jbm.b.33826] [PMID: 28106947]

[109]
Adepu S, Dhiman N, Laha A, Sharma CS, Ramakrishna S, Khandelwal M. Three-dimensional bioprinting for bone tissue regeneration. Curr Opin Biomed Eng  2017; 2: 22-8.
 [http://dx.doi.org/10.1016/j.cobme.2017.03.005]

[110]
Griffin KS, Davis KM, McKinley TO, et al. Evolution of bone grafting: Bone grafts and tissue engineering strategies for vascularized bone regeneration. Clin Rev Bone Miner Metab  2015; 13(4): 232-44.
 [http://dx.doi.org/10.1007/s12018-015-9194-9]

[111]
Kumar S, Wan C, Ramaswamy G, Clemens TL, Ponnazhagan S. Mesenchymal stem cells expressing osteogenic and angiogenic factors synergistically enhance bone formation in a mouse model of segmental bone defect. Mol Ther  2010; 18(5): 1026-34.
 [http://dx.doi.org/10.1038/mt.2009.315] [PMID: 20068549]
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DOI https://dx.doi.org/10.2174/1574888X18666230530153521	Print ISSN 1574-888X
Publisher Name Bentham Science Publisher	Online ISSN 2212-3946
Current Stem Cell Research & Therapy

Recent Approaches to Enhance Osteogenesis of Dental Pulp Stem Cells on Electrospun Scaffolds

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