Login Register Cart 0
Generic placeholder image

Current HIV Research

Editor-in-Chief

ISSN (Print): 1570-162X
ISSN (Online): 1873-4251

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Point-of-Care HIV Test for a Promising Simple and Rapid Clinical HIV Definite Diagnosis Process

Author(s): Zhenrui Xue, Min Song, Ping Peng and Chunyan Yao*

Volume 21, Issue 2, 2023

Published on: 28 March, 2023

Page: [117 - 121] Pages: 5

DOI: 10.2174/1570162X21666230309115137

Price: $65

Abstract

Background: This study compared and evaluated the performance of a commercially available HIV POC rapid test with assays commonly used in clinical laboratories, including enzymelinked immunosorbent assay (ELISA), western blot (WB), and reverse transcription-polymerase chain reaction (RT-PCR).

Methods: 500 patients’ samples were detected by the POC rapid test and clinically common tests (WB, ELISA, and RT-PCR) to compare detection performance, test time, and test cost.

Results: Taking the WB results as the gold standard, the results of RT-PCR were completely consistent with WB. The concordance of ELISA and POC with WB was 82.00% and 93.80%, respectively, with statistically significant differences (p<0.05).

Conclusion: This study provides evidence that rapid HIV POC assays are superior to ELISA and that WB and RT-PCR have equal detection performance in detecting HIV. As a result, a rapid and costeffective HIV definition process based on the POC assays can be proposed.

« Previous Next »
Graphical Abstract

[1]
Yu Y, Qin K, Xiao F, et al. HIV disclosure among sexually infected people living with HIV and AIDS in China: Prevalence, influencing factors, and negative outcomes. AIDS Behav 2022; 1-19.
[http://dx.doi.org/10.1007/s10461-022-03968-z] [PMID: 36580165]
[2]
Nandi S, Mondal A, Roberts A, Gandhi S. Biosensor platforms for rapid HIV detection. Adv Clin Chem 2020; 98: 1-34.
[http://dx.doi.org/10.1016/bs.acc.2020.02.001] [PMID: 32564784]
[3]
Mehra B, Bhattar S, Bhalla P, Rawat D. Rapid tests versus ELISA for screening of HIV infection: Our experience from a voluntary counselling and testing facility of a tertiary care centre in North India. ISRN AIDS 2014; 2014: 1-5.
[http://dx.doi.org/10.1155/2014/296840] [PMID: 25006527]
[4]
Zaini R, Shesha N. Comparison between the accuracy of a rapid point-of-care HIV test TOYO versus ELISA, Western Blot, and RT-PCR analysis. Int J Med Sci Public Health 2016; 5(5): 877.
[http://dx.doi.org/10.5455/ijmsph.2016.25122015304]
[5]
Alaidarous M, Choudhary RK, Waly MI, et al. The prevalence of transfusion-transmitted infections and nucleic acid testing among blood donors in Majmaah, Saudi Arabia. J Infect Public Health 2018; 11(5): 702-6.
[http://dx.doi.org/10.1016/j.jiph.2018.04.008] [PMID: 29703711]
[6]
Xu S, Huang W, Zhang L, et al. Regulation and quality evaluation system for HIV diagnostics in China. Biologicals 2016; 44(2): 111-6.
[http://dx.doi.org/10.1016/j.biologicals.2015.12.004] [PMID: 26811217]
[7]
Minichiello A, Swab M, Chongo M, et al. HIV point-of-care testing in canadian settings: A scoping review. Front Public Health 2017; 5: 76.
[http://dx.doi.org/10.3389/fpubh.2017.00076] [PMID: 28459048]
[8]
Marinho FLO, Santos NLL, Neves SPF, Vasconcellos LS. Performance evaluation of eight rapid tests to detect HIV infection: A comparative study from Brazil. PLoS One 2020; 15(8): e0237438.
[http://dx.doi.org/10.1371/journal.pone.0237438] [PMID: 32790799]
[9]
Panneer N, Lontok E, Branson BM, et al. HIV and hepatitis C virus infection in the United States: whom and how to test. Clin Infect Dis 2014; 59(6): 875-82.
[http://dx.doi.org/10.1093/cid/ciu396] [PMID: 24867787]
[10]
Bystryak S, Acharya C. Detection of HIV-1 p24 antigen in patients with varying degrees of viremia using an ELISA with a photochemical signal amplification system. Clin Chim Acta 2016; 456: 128-36.
[http://dx.doi.org/10.1016/j.cca.2016.02.022] [PMID: 26940950]
[11]
Ran B, Xianyu Y, Dong M, Chen Y, Qian Z, Jiang X. Bioorthogonal reaction-mediated ELISA using peroxide test strip as signal readout for point-of-care testing. Anal Chem 2017; 89(11): 6113-9.
[http://dx.doi.org/10.1021/acs.analchem.7b00831] [PMID: 28460169]
[12]
Chen H, Liu K, Li Z, Wang P. Point of care testing for infectious diseases. Clin Chim Acta 2019; 493: 138-47.
[http://dx.doi.org/10.1016/j.cca.2019.03.008] [PMID: 30853460]
[13]
Moodley D, Moodley P, Ndabandaba T, Esterhuizen T. Reliability of HIV rapid tests in a clinical setting is largely dependent on user performance. S Afr Med J 2008; 98(9): 707-9.
[PMID: 19113051]
[14]
Meulbroek M, Pujol F, Pérez F, et al. BCN Checkpoint: Same-day confirmation of reactive HIV rapid test with Point Of Care HIV-RNA accelerates linkage to care and reduces anxiety. HIV Med 2018; 19(S1): 63-5.
[http://dx.doi.org/10.1111/hiv.12595] [PMID: 29488706]
[15]
Demorat H, Lopes A, Chopin D, et al. Acceptability and feasibility of HIV testing in general medicine by ELISA or rapid test from finger-stick whole blood. Presse Med 2018; 47(2): e15-23.
[http://dx.doi.org/10.1016/j.lpm.2017.11.022] [PMID: 29478794]
[16]
Wolpaw BJ, Mathews C, Chopra M, et al. The failure of routine rapid HIV testing: A case study of improving low sensitivity in the field. BMC Health Serv Res 2010; 10(1): 73.
[http://dx.doi.org/10.1186/1472-6963-10-73] [PMID: 20307310]
[17]
Bhore AV, Sastry J, Patke D, et al. Sensitivity and specificity of rapid HIV testing of pregnant women in India. Int J STD AIDS 2003; 14(1): 37-41.
[http://dx.doi.org/10.1258/095646203321043246] [PMID: 12590791]
[18]
Manenzhe SC, Ngwenya SP, Shangase SL. The diagnostic accuracy of the HIV 1/2/subtype O Tri‐line rapid test compared with ELISA: A pilot study. Oral Dis 2020; 26(S1): 161-4.
[http://dx.doi.org/10.1111/odi.13399] [PMID: 32862539]
[19]
Liu MQ, Zhu ZR, Kong WH, et al. High rate of missed HIV infections in individuals with indeterminate or negative HIV western blots based on current HIV testing algorithm in China. J Med Virol 2016; 88(8): 1462-6.
[http://dx.doi.org/10.1002/jmv.24490] [PMID: 26856240]
[20]
Branson BM, Owen SM, Wesolowski LG, et al. Laboratory testing for the diagnosis of HIV infection: updated recommendations Maryland USA: Centers for Disease Control and Prevention. (U.S.): Association of Public Health Laboratories 2014.
[http://dx.doi.org/10.15620/cdc.23447]
[21]
Bennett B, Branson BM, Ethridge SF, et al. Suggested Reporting Language for the HIV Laboratory Diagnostic Testing Algorithm Association of Public Health Laboratories. 2013. Available from: https://stacks.cdc.gov/view/cdc/22423
[22]
Safavieh M, Kaul V, Khetani S, et al. Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens. Nanoscale 2017; 9(5): 1852-61.
[http://dx.doi.org/10.1039/C6NR06417E] [PMID: 27845796]
[23]
Kong M, Li Z, Wu J, et al. A wearable microfluidic device for rapid detection of HIV-1 DNA using recombinase polymerase amplification. Talanta 2019; 205: 120155.
[http://dx.doi.org/10.1016/j.talanta.2019.120155] [PMID: 31450450]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy