Preparation of protected peptides by gel-phase synthesis on butanediol dimethacrylate cross-linked polystyrene support

Title:Preparation of protected peptides by gel-phase synthesis on butanediol dimethacrylate cross-linked polystyrene support

Volume: 7 Issue: 6

Author(s): M. Roice and V. N. Rajasekharan Pillai

Affiliation:

Abstract: Preparation of fully protected peptide C-terminal esters in high yield and purity by making use of gel-phase synthesis on chloromethyl butanediol dimethacrylate (BDODMA) crosslinked polystyrene is described. The C-terminal amino acid of the peptide was incorporated by cesium salt method and the step-wise synthesis was carried out using HOBt active ester coupling procedure. The protected peptides were cleaved from the support by trans-esterification. The crude peptides were purified by HPLC and characterized by amino acid analysis, tic and MALDI TOF MS.

Cite this article as:

Roice M. and Pillai Rajasekharan V. N., Preparation of protected peptides by gel-phase synthesis on butanediol dimethacrylate cross-linked polystyrene support, Protein & Peptide Letters 2000; 7 (6) . https://dx.doi.org/10.2174/092986650706221207161246