Login Register Cart 0
Generic placeholder image

Letters in Drug Design & Discovery

Editor-in-Chief

ISSN (Print): 1570-1808
ISSN (Online): 1875-628X

Back
Research Article

The Therapeutic Potential of Panax Ginseng and Ginger on Postsurgical Adhesion Band Formation

Author(s): Seyedeh Elnaz Nazari, Ghazaleh Khalili-Tanha, Leila Mobasheri, Fereshteh Asgharzadeh, Hamideh Naimi, Moein Eskandari, Mohammad-Mostafa Askarnia-Faal, Seyed Mahdi Hassanian Mehr, Masoumeh Gharib, Hamid Reza Ghorbani, Mohsen Aliakbarian, Gordon A Ferns, Majid Khazaei* and Amir Avan*

Volume 20, Issue 10, 2023

Published on: 25 August, 2022

Page: [1585 - 1592] Pages: 8

DOI: 10.2174/1570180819666220630094320

Price: $65

Abstract

Background: Peritoneal adhesions (PA) are a common complication of abdominal operations. Previous studies indicate that inhibition of inflammation and fibrosis at sites of peritoneal damage may prevent the development of intra-abdominal adhesions. Zingiber officinalis Roscoe (ginger) and Panax ginseng (P. ginseng) are herbal products with antioxidant and anti-inflammatory effects that can have restorative properties.

Objective: This research aimed to examine the impact of ginger and P. ginseng on prevention of PA in a rat model after surgery.

Methods: Following a laparotomy, the wall of the cecum was rubbed to induce intra-abdominal adherence in Wistar rats. Ginger (400mg/kg) and P. ginseng (500mg/kg) were orally adminstered to the animals. The animals were sacrificed on the 10th day after surgery, and the Nair and Leach scoring system was used to assess adhesion. The microscopic histology of the induced cecal adhesions was evaluated. An enzyme-linked immunosorbent assay (ELISA) was used to determined tissue levels of transforming growth factor-beta (TGF-β) on homogenized PA tissue. Real-time PCR was performed to quantify the mRNA expression of IL-1β, TNF-α, Col 1a1, and Col 3a1 in rat tissue.

Results: The adhesion score and histopathological rating based on the Nair and Leach scoring criteria showed lower adhesion scores in the group of rats treated with P. ginseng compared to the control group (p<0.05). Treatment with ginger and P. ginseng was associated with reduced tissue level of TGF-β and mRNA expression of TNF-α and IL-1β. The gene expression level of Col 1a1 and Col 3a1 were markedly decreased in the P. ginseng group.

Conclusion: The study's outcome recommends that P. ginseng could be an effective agent for preventing the PA and inflammation during the post-operative stage.

Keywords: postoperative adhesions, panax ginseng, ginger, inflammation, fibrosis

« Previous Next »
Graphical Abstract

[1]
ten Broek, R.P.; Bakkum, E.A.; Laarhoven, C.J.; van Goor, H. Epidemiology and prevention of postsurgical adhesions revisited. Ann. Surg., 2016, 263(1), 12-19.
[http://dx.doi.org/10.1097/SLA.0000000000001286] [PMID: 26135678]
[2]
Vediappan, R.S.; Bennett, C.; Bassiouni, A.; Smith, M.; Finnie, J.; Trochsler, M.; Psaltis, A.J.; Vreugde, S.; Wormald, P.J. A novel rat model to test intra-abdominal anti-adhesive therapy. Front. Surg., 2020, 7, 12.
[http://dx.doi.org/10.3389/fsurg.2020.00012] [PMID: 32322586]
[3]
Schnüriger, B.; Barmparas, G.; Branco, B.C.; Lustenberger, T.; Inaba, K.; Demetriades, D. Prevention of postoperative peritoneal adhesions: A review of the literature. Am. J. Surg., 2011, 201(1), 111-121.
[http://dx.doi.org/10.1016/j.amjsurg.2010.02.008] [PMID: 20817145]
[4]
Strik, C.; Wever, K.E.; Stommel, M.W.J.; Goor, H.V.; Ten Broek, R.P.G. Adhesion reformation and the limited translational value of experiments with adhesion barriers: A systematic review and meta-analysis of animal models. Sci. Rep., 2019, 9(1), 18254.
[http://dx.doi.org/10.1038/s41598-019-52457-2] [PMID: 31796777]
[5]
Charboneau, A.J.; Delaney, J.P.; Beilman, G. Fucoidans inhibit the formation of post-operative abdominal adhesions in a rat model. PLoS One, 2018, 13(11), e0207797.
[http://dx.doi.org/10.1371/journal.pone.0207797] [PMID: 30462732]
[6]
Gao, Q.; Wei, G.; Wu, Y.; Yao, N.; Zhou, C.; Wang, K.; Wang, K.; Sun, X.; Li, X. Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model. Oncotarget, 2017, 8(55), 93899-93911.
[http://dx.doi.org/10.18632/oncotarget.21333] [PMID: 29212197]
[7]
Javanmardi, S.; Golmohammadi, S.; Mazaheri-Khameneh, R. Evaluation of Silymarin effects on post-operative peritoneal adhesion in Rats. The Urmia University of Medical Sciences, 2017, 28(8), 55-63.
[8]
Askari, V.R.; Rahimi, V.B.; Zamani, P.; Fereydouni, N.; Rahmanian-Devin, P.; Sahebkar, A.H.; Rakhshandeh, H. Evaluation of the effects of Iranian propolis on the severity of post operational-induced peritoneal adhesion in rats. Biomed. Pharmacother., 2018, 99, 346-353.
[http://dx.doi.org/10.1016/j.biopha.2018.01.068] [PMID: 29665643]
[9]
Tungmunnithum, D.; Thongboonyou, A.; Pholboon, A.; Yangsabai, A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines (Basel), 2018, 5(3), 93.
[http://dx.doi.org/10.3390/medicines5030093] [PMID: 30149600]
[10]
Mao, Q-Q.; Xu, X-Y.; Cao, S-Y.; Gan, R-Y.; Corke, H.; Beta, T.; Li, H.B. Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods, 2019, 8(6), 185.
[http://dx.doi.org/10.3390/foods8060185] [PMID: 31151279]
[11]
Liu, Y.; Liu, J.; Zhang, Y. Research progress on chemical constituents of Zingiber officinale Roscoe. Bio. Med. Res. Inter, 2019, 2019
[http://dx.doi.org/10.1155/2019/5370823]
[12]
Al Hroob, A.M.; Abukhalil, M.H.; Alghonmeen, R.D.; Mahmoud, A.M. Ginger alleviates hyperglycemia-induced oxidative stress, inflammation and apoptosis and protects rats against diabetic nephropathy. Biomed. Pharmacother., 2018, 106, 381-389.
[http://dx.doi.org/10.1016/j.biopha.2018.06.148] [PMID: 29966984]
[13]
Kim, J-H. Pharmacological and medical applications of Panax ginseng and ginsenosides: A review for use in cardiovascular diseases. J. Ginseng Res., 2018, 42(3), 264-269.
[http://dx.doi.org/10.1016/j.jgr.2017.10.004] [PMID: 29983607]
[14]
Kang, S.; Min, H. Ginseng, the’immunity boost’: The effects of Panax ginseng on immune system. J. Ginseng Res., 2012, 36(4), 354-368.
[http://dx.doi.org/10.5142/jgr.2012.36.4.354] [PMID: 23717137]
[15]
Chang, Y.; Lai, P.-H.; Wang, C.-C.; Chen, S.-C.; Chang, W.-C.; Sung, H.-W. Mesothelium regeneration on acellular bovine pericardia loaded with an angiogenic agent (ginsenoside Rg1) successfully reduces postsurgical pericardial adhesions J. Thora. Cardiovas Surg., 2006, 132(4), 867-874.e3.
[16]
Liu, R.; Chen, Q-H.; Ren, J-W.; Sun, B.; Cai, X-X.; Li, D.; Mao, R-X.; Wu, X.; Li, Y. Ginseng (Panax ginseng Meyer) oligopeptides protect against binge drinking-induced liver damage through inhibiting oxidative stress and inflammation in rats. Nutrients, 2018, 10(11), 1665.
[http://dx.doi.org/10.3390/nu10111665] [PMID: 30400371]
[17]
Karunasagara, S.; Hong, G-L.; Park, S-R.; Lee, N-H.; Jung, D-Y.; Kim, T-W.; Jung, J-Y. Korean red ginseng attenuates hyperglycemia-induced renal inflammation and fibrosis via accelerated autophagy and protects against diabetic kidney disease. J. Ethnopharmacol., 2020, 254, 112693.
[http://dx.doi.org/10.1016/j.jep.2020.112693] [PMID: 32112899]
[18]
Afshari, A.T.; Shirpoor, A.; Farshid, A.; Saadatian, R.; Rasmi, Y.; Saboory, E.; Ilkhanizadeh, B.; Allameh, A. The effect of ginger on diabetic nephropathy, plasma antioxidant capacity and lipid peroxidation in rats. Food Chem., 2007, 101(1), 148-153.
[http://dx.doi.org/10.1016/j.foodchem.2006.01.013]
[19]
Abdi, T.; Mahmoudabady, M.; Marzouni, H.Z.; Niazmand, S.; Khazaei, M. Ginger (Zingiber Officinale Roscoe) extract protects the heart against inflammation and fibrosis in diabetic rats. Can. J. Diabetes, 2021, 45(3), 220-227.
[http://dx.doi.org/10.1016/j.jcjd.2020.08.102] [PMID: 33162372]
[20]
Nair, S.K.; Bhat, I.K.; Aurora, A.L. Role of proteolytic enzyme in the prevention of postoperative intraperitoneal adhesions. Arch. Surg., 1974, 108(6), 849-853.
[http://dx.doi.org/10.1001/archsurg.1974.01350300081019] [PMID: 4829809]
[21]
Leach, R.E.; Burns, J.W.; Dawe, E.J. SmithBarbour, M.D.; Diamond, M.P. Reduction of postsurgical adhesion formation in the rabbit uterine horn model with use of hyaluronate/carboxymethylcellulose gel. Fertil. Steril., 1998, 69(3), 415-418.
[http://dx.doi.org/10.1016/S0015-0282(97)00573-6] [PMID: 9531869]
[22]
Wei, G.; Wu, Y.; Gao, Q.; Shen, C.; Chen, Z.; Wang, K.; Yu, J.; Li, X.; Sun, X. Gallic acid attenuates postoperative intra-abdominal adhesion by inhibiting inflammatory reaction in a rat model. Med. Sci. Monit., 2018, 24, 827-838.
[http://dx.doi.org/10.12659/MSM.908550] [PMID: 29429982]
[23]
Wu, F.; Liu, W.; Feng, H.; Long, L.; Hou, L.; Hou, C. Application of traditional Chinese medicines in postoperative abdominal adhesion Evid.based Complement. Altern. Med., 2020, 2020
[http://dx.doi.org/10.1155/2020/8073467]
[24]
Gumán-Valdivia-Gómez, G.; Tena-Betancourt, E.; de Alva-Coria, P.M. Postoperative abdominal adhesions: Pathogenesis and current preventive techniques. Cir. Cir., 2019, 87(6), 698-703.
[PMID: 31631189]
[25]
Parsaei, P.; Karimi, M.; Asadi, S.Y.; Rafieian-Kopaei, M. Bioactive components and preventive effect of green tea (Camellia sinensis) extract on post-laparotomy intra-abdominal adhesion in rats. Int. J. Surg., 2013, 11(9), 811-815.
[http://dx.doi.org/10.1016/j.ijsu.2013.08.014] [PMID: 23994005]
[26]
Karimi, M.; Yazdan Asadi, S.; Parsaei, P.; Rafieian-Kopaei, M.; Ghaheri, H.; Ezzati, S. The effect of ethanol extract of rose (rosa damascena) on intra-abdominal adhesions after laparotomy in rats. Wounds, 2016, 28(5), 167-174.
[PMID: 27191175]
[27]
Bayhan, Z.; Zeren, S.; Kocak, F.E.; Kocak, C. Akcılar, R.; Kargı E.; Tiryaki, C.; Yaylak, F.; Akcılar, A. Antiadhesive and anti-inflammatory effects of pirfenidone in postoperative intra-abdominal adhesion in an experimental rat model. J. Surg. Res., 2016, 201(2), 348-355.
[28]
Rahimi, V.B.; Shirazinia, R.; Fereydouni, N.; Zamani, P.; Darroudi, S.; Sahebkar, A.H.; Askari, V.R. Comparison of honey and dextrose solution on post-operative peritoneal adhesion in rat model. Biomed. Pharmacother., 2017, 92, 849-855.
[http://dx.doi.org/10.1016/j.biopha.2017.05.114] [PMID: 28618654]
[29]
Roohbakhsh, Y.; Baradaran Rahimi, V.; Silakhori, S.; Rajabi, H.; Rahmanian-Devin, P.; Samzadeh-Kermani, A.; Rakhshandeh, H.; Hasanpour, M.; Iranshahi, M.; Mousavi, S.H.; Askari, V.R. Evaluation of the effects of peritoneal lavage with rosmarinus officinalis extract against the prevention of postsurgical-induced peritoneal adhesion. Planta Med., 2020, 86(6), 405-414.
[http://dx.doi.org/10.1055/a-1118-3918] [PMID: 32097974]
[30]
Barrientos, S.; Stojadinovic, O.; Golinko, M.S.; Brem, H.; Tomic-Canic, M. Growth factors and cytokines in wound healing. Wound Repair Regen., 2008, 16(5), 585-601.
[http://dx.doi.org/10.1111/j.1524-475X.2008.00410.x] [PMID: 19128254]
[31]
Herrick, S.E.; Wilm, B. Post-surgical peritoneal scarring and key molecular mechanisms. Biomolecules, 2021, 11(5), 692.
[http://dx.doi.org/10.3390/biom11050692] [PMID: 34063089]
[32]
Flevaris, P.; Vaughan, D. The role of plasminogen activator inhibitor type-1 in fibrosis, Seminars in thrombosis and hemostasis; Thieme Medical Publishers, 2017, pp. 169-177.
[33]
Sanjabi, S.; Zenewicz, L.A.; Kamanaka, M.; Flavell, R.A. Anti-inflammatory and pro-inflammatory roles of TGF-β IL-10, and IL-22 in immunity and autoimmunity. Curr. Opin. Pharmacol., 2009, 9(4), 447-453.
[http://dx.doi.org/10.1016/j.coph.2009.04.008] [PMID: 19481975]

Rights & Permissions Print Cite
Article Metrics
3
© 2024 Bentham Science Publishers | Privacy Policy