Construction of a Competitive Endogenous RNA Network Related to Exosomes
in Diabetic Retinopathy

Tong      Wang; Mao-yuan      Cheng; Meng-ya      Shan; Cui-yao      Tang; Nai-jia      Pan; Zi-hui      Xu; Xian-lun      Tang
doi:10.2174/1386207325666220610122114
Abstract

Background: The competing endogenous RNA (ceRNA) network plays an important role in the occurrence and development of a variety of diseases. This study aimed to construct a ceRNA network related to exosomes in diabetic retinopathy (DR).
Methods: We explored the Gene Expression Omnibus (GEO) database and then analyzed the RNAs of samples to obtain differentially expressed lncRNAs (DELs), miRNAs (DEMs) and mRNAs (DEGs) alongside the progress of DR. Next, Gene Set Enrichment Analysis (GSEA) analysis of DEGs, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of up-DEGs were performed. In addition, a ceRNA network related to exosomes in DR was constructed on the base of DELs, DEMs and DEGs. Finally, the function of the ceRNA network was explored by GO and KEGG enrichment analysis.
Results: Through our analysis, 267 DELs (93 up and 174 down), 114 DEMs (64 up and 50 down) and 2368 DEGs (1252 up and 1116 down) were screened. The GSEA analysis results show that these genes were mainly related to cytokine-cytokine receptor interaction, hippo signaling pathway and JAK-STAT signaling pathway. The GO and KEGG results show that these up-DEGs were mainly enriched in viral gene expression, components of ribosomes, mineral absorption, Wntprotein binding, and TGF-β signaling pathway. Besides, a ceRNA network, including 15 lncRNAs (e.g., C1orf145, FGF14-IT1, and PRNT), 3 miRNAs (miR-10a-5p, miR-1297 and miR-507) and 11 mRNAs (NCOR2, CHAC1 and LIX1L, etc.) was constructed. Those 5 lncRNAs were up-regulated, 1 miRNA was down-regulated and 5 mRNAs were up-regulated in DR, while 10 lncRNAs were downregulated, 2 miRNAs were up-regulated and 6 mRNAs were down-regulated in DR.
Conclusion: The novel ceRNA network that we constructed will provide new insights into the underlying molecular mechanisms of exosomes in DR.
Keywords: Bioinformatics analysis, ceRNA network, exosomes, diabetic retinopathy
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Graphical Abstract

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DOI https://dx.doi.org/10.2174/1386207325666220610122114	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402
Combinatorial Chemistry & High Throughput Screening

Construction of a Competitive Endogenous RNA Network Related to Exosomes in Diabetic Retinopathy

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