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Current Medicinal Chemistry

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ISSN (Print): 0929-8673
ISSN (Online): 1875-533X

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Research Article

Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors

Author(s): Bruno P. Crulhas, Caroline R. Basso, Gustavo R. Castro and Valber A. Pedrosa*

Volume 29, Issue 37, 2022

Published on: 04 August, 2022

Page: [5895 - 5902] Pages: 8

DOI: 10.2174/0929867329666220607162250

Price: $65

Abstract

Background: Prostate cancer cells have very high PCA3 messenger RNA levels, which turns them into one of the new biomarkers for prostate cancer prognosis and diagnosis.

Objective: Our goal here is to develop a new aptasensor to detect PCA3 release by the cancer cell.

Methods: DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methylene blue (MB) redox probe, and immobilized on gold electrode through self-assembly to detect label-free cancer cells.

Results: Our data have evidenced stable and sensitive sensors presenting a wide linear detection range (0-150ng/mL). In addition, monitoring PCA3 released by different types of prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore, it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary depending on the type of adopted prostate cells.

Conclusion: PCA3 release was monitored in a group of cells for 2 h; it showed significantly higher expression in both LNCaP and PC-3 cells. This strategy provides a unique and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it emerged as a promising tool for early cost-effective diagnosis.

Keywords: Aptasensor, releasing protein, PCA3, aptamer, cancer cell, biomarker.

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