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Current Pharmaceutical Design

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ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

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Research Article

GC-MS Identification and Quantification of the Synthetic Cannabinoid MDMB-4en- PINACA in Cannabis-derived Material Seized in the Turin Metropolitan Area (Italy)

Author(s): Enrico Gerace*, Fabrizio Seganti, Daniele Di Corcia, Marco Vincenti and Alberto Salomone

Volume 28, Issue 32, 2022

Published on: 22 June, 2022

Page: [2618 - 2621] Pages: 4

DOI: 10.2174/1381612828666220603142859

Price: $65

Abstract

Background: The presence of the synthetic cannabinoid receptor agonist MDMB-4en-PINACA in adulterated low-THC cannabis products was recently highlighted in several reports. Moreover, numerous intoxication cases involving MDMB-4en-PINACA have been described.

Objective: In order to monitor the diffusion of cannabis products containing MDMB-4en-PINACA in our territory, a total of 358 cannabis-derived samples (213 vegetal material and 145 resins) seized in the period November 2020 - February 2021 in the western Piedmont Area (Italy) was analyzed.

Methods: General screening analyses for traditional and synthetic cannabinoids were performed by a GC-MS device operating in full scan mode (40-600 amu). The MDMB-4en-PINACA was quantified by means of a specific GC-SIM-MS protocol purposely developed and validated, while the quantification of THC, CBD, and CBN was carried out by a GC-SIM-MS method routinely employed in our laboratory.

Results: MDMB-4en-PINACA was detected in 12 out of 358 samples (3.4% of the total). Among these, the molecule was found in 11 vegetal materials and in one resin sample. Considering solely the analysis of the 213 herb products, a positive rate of 5.2% was found for the presence of MDMB-4en-PINACA in these samples. MDMB-4en-PINACA was found in the seized materials at concentration levels ranging from 0.4 up to 6.3 mg/g (mean 2.5 mg/g; median 1.7 mg/g). Concerning the traditional cannabinoids, the THC concentration was in the interval 3-43 mg/g (mean 12 mg/g; median 7 mg/g), while CBD was found at higher concentrations in all specimens, specifically in the range 47-140 mg/g (mean 87 mg/g; median 80 mg/g).

Conclusion: The adulteration of low-THC cannabis products with synthetic cannabinoid receptor agonists is widespread today. Since these substances are potentially more toxic than THC, their consumption poses a high risk of overdose for unaware users and a health-threatening situation. This study confirmed the sporadic presence on the market of CBD-prevalent cannabis products adulterated with MDMB-4en-PINACA.

Keywords: Synthetic cannabinoids, cannabis, adulterated, MDMB-4en-PINACA, NPS, GC-MS.

« Previous Next »
[1]
European drug report: trends and developments. Luxembourg: Publications office of the European Union; EMCDDA 2021. Available from: https://www.emcdda.europa.eu/system/files/ publications/13838/TDAT21001ENN.pdf (Accessed on December 27, 2021).
[2]
Synthetic cannabinoids in Europe – a review. Luxembourg: Publications office of the European Union; EMCDDA 2021. Available from: https://www.emcdda.europa.eu/system/files/publications/14035/Synthetic-cannabinoids-in-Europe-EMCDDA-technical-report.pdf (Accessed on December 27, 2021).
[3]
Evans-Brown M, Sedefov R. Responding to new psychoactive substances in the European union: early warning, risk assessment, and control measures. In: In: New Psychoactive Substances: Pharmacology, Clinical, Forensic and Analytical Toxicology Maurer HH, Brandt SD Cham: Springer International Publishing. 2018; p. pp. 3-49.
[http://dx.doi.org/10.1007/164_2018_160]
[4]
Apirakkan O, Frinculescu A, Denton H, et al. Isolation, detection and identification of synthetic cannabinoids in alternative formulations or dosage forms. Forensic Chem 2020; 18100227
[http://dx.doi.org/10.1016/j.forc.2020.100227]
[5]
Rodrigues TB, Souza MP, de Melo Barbosa L, et al. Synthetic cannabinoid receptor agonists profile in infused papers seized in Brazilian prisons. Forensic Toxicol 2021. Available from: https://link.springer.com/10.1007/s11419-021-00586-7 (Accessed on December 27, 2021).
[6]
Oomen PE, Schori D, Tögel-Lins K, et al. Cannabis adulterated with the synthetic cannabinoid receptor agonist MDMB-4en-PINACA and the role of European drug checking services. Int J Drug Policy 2022; 100103493
[http://dx.doi.org/10.1016/j.drugpo.2021.103493] [PMID: 34687992]
[7]
Manthey J. Cannabis use in Europe: Current trends and public health concerns. Int J Drug Policy 2019; 68: 93-6.
[http://dx.doi.org/10.1016/j.drugpo.2019.03.006] [PMID: 31030057]
[8]
Hazekamp A. The trouble with CBD oil. Med Cannabis Cannabinoids 2018; 1(1): 65-72.
[http://dx.doi.org/10.1159/000489287] [PMID: 34676324]
[9]
De Morais J, Brandt SD, Jorge R, et al. EMCDDA technical report on the new psychoactive substance methyl 3,3-dimethyl-2-{[1-(pent- 4-en-1-yl)-1H-indazole-3-carbonyl]amino}butanoate (MDMB-4en- PINACA). European Monitoring Centre for Drugs and Drug Addiction 2020. Available from: https://www.emcdda.europa.eu/ publications/technical-reports/technical-report-mdmb-4en-pinaca_ru (Accessed on December 27, 2021).
[10]
Erol Ozturk Y, Yeter O. In vitro phase I metabolism of the recently emerged synthetic mdmb-4en-pinaca and its detection in human urine samples. J Anal Toxicol 2021; 44(9): 976-84.
[http://dx.doi.org/10.1093/jat/bkaa017] [PMID: 32091101]
[11]
Liu CM, Jia W, Meng X, Hua ZD. Identification and quantification of 10 indole/indazole carboxamide synthetic cannabinoids in 36 herbal blends by gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. J Forensic Sci 2021; 66(6): 2156-66.
[http://dx.doi.org/10.1111/1556-4029.14873] [PMID: 34431514]
[12]
Norman C, Walker G, McKirdy B, et al. Detection and quantitation of synthetic cannabinoid receptor agonists in infused papers from prisons in a constantly evolving illicit market. Drug Test Anal 2020; 12(4): 538-54.
[http://dx.doi.org/10.1002/dta.2767] [PMID: 31944624]
[13]
Cannaert A, Sparkes E, Pike E, et al. Synthesis and in vitro cannabinoid receptor 1 activity of recently detected synthetic cannabinoids 4F-MDMB-BICA, 5F-MPP-PICA, MMB-4en-PICA, CUMYL-CBMICA, ADB-BINACA, APP-BINACA, 4F-MDMB-BINACA, MDMB-4en-PINACA, A-CHMINACA, 5F-AB-P7AICA, 5F-MDMB-P7AICA, and 5F-AP7AICA. ACS Chem Neurosci 2020; 11(24): 4434-46.
[http://dx.doi.org/10.1021/acschemneuro.0c00644] [PMID: 33253529]
[14]
Krotulski AJ, Cannaert A, Stove C, Logan BK. The next generation of synthetic cannabinoids: Detection, activity, and potential toxicity of pent-4en and but-3en analogues including MDMB-4en-PINACA. Drug Test Anal 2021; 13(2): 427-38.
[http://dx.doi.org/10.1002/dta.2935] [PMID: 32997377]
[15]
Goncalves R, Labadie M, Chouraqui S, et al. Involuntary MDMB-4en-PINACA intoxications following cannabis consumption: clinical and analytical findings. Clin Toxicol (Phila) 2022; 60(4): 458-63.
[http://dx.doi.org/10.1080/15563650.2021.1994144] [PMID: 34850659]

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