Login Register Cart 0
Generic placeholder image

Current Stem Cell Research & Therapy

Editor-in-Chief

ISSN (Print): 1574-888X
ISSN (Online): 2212-3946

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

The Effect of Injection of Secretome of Umbilical Cord Mesenchymal Stem Cells in Articular Cartilage Repair in Sheep Model

Author(s): Andri M.T. Lubis*, Andi Praja Wira Yudha Luthfi, Jeanne Adiwinata Pawitan, Bambang Pontjo Priosoeryanto and Anissa Feby Canintika

Volume 18, Issue 4, 2023

Published on: 07 September, 2022

Page: [522 - 527] Pages: 6

DOI: 10.2174/1574888X17666220426114841

Price: $65

Abstract

Introduction: Articular cartilage is an avascular, aneural, and lymphatic tissue with limited capacity to regenerate. Numerous techniques have been employed to repair or regenerate; however, the success rate varies. In fact, most of them result in the formation of fibrocartilage, not hyaline cartilage. The future of treating cartilage defects lies in providing biological solutions through cartilage regeneration. Mesenchymal stem cells (MSCs) represent a promising therapy for cartilage regeneration. These cells secrete factors that enhance cartilage repair. This study studied the effects of intra-articular injection of human umbilical cord MSC (hUC-MSC) secretome on cartilage damage in a sheep model.

Methods: Standardized rectangular (5x5 mm) full-thickness chondral defects were created in the lateral femoral condyle of 15 adult sheep and debrided down to the subchondral bone plate. Three treatment groups were tested: 4 microfracture perforations using 1.0mm diameter awls (group 1), intra-articular injection of hUC-MSC secretome (group 2), and a combination of microfracture and intra-articular injection of hUC-MSC secretome (group 3). The osteochondral repair was assessed at 6 months using an established macroscopic and histological analyses.

Results: Macroscopically, combined therapy application shows significant cartilage repair improvement compared to microfracture alone (p=0.004). Microscopically, the application of combined therapy shows significant improvement of cartilage repair compared to secretome injection alone (p=0.031).

Conclusion: Microfracture combined with injection of hUCB-MSCs secretome could be an effective alternative for repairing articular cartilage defects in vivo.

Keywords: Secretome, articular cartilage, sheep, stem cells, microfracture, secretome.

« Previous Next »
Graphical Abstract

[1]
Deng Z, Jin J, Zhao J, Xu H. Cartilage defect treatments: With or without cells? Mesenchymal stem cells or chondrocytes? Traditional or matrix-assisted? A systematic review and meta-analyses. Stem Cells Int 2016; 2016: 1-14.
[http://dx.doi.org/10.1155/2016/9201492] [PMID: 26839570]
[2]
Memon AR, Quinlan JF. Surgical treatment of articular cartilage defects in the knee: Are we winning? Adv Orthop 2012; 2012: 1-6.
[http://dx.doi.org/10.1155/2012/528423] [PMID: 22655202]
[3]
Dewan AK, Gibson MA, Elisseeff JH, Trice ME. Evolution of autologous chondrocyte repair and comparison to other cartilage repair techniques. BioMed Res Int 2014; 2014: 272481.
[http://dx.doi.org/10.1155/2014/272481] [PMID: 25210707]
[4]
Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Prolif 1970; 3(4): 393-403.
[http://dx.doi.org/10.1111/j.1365-2184.1970.tb00347.x] [PMID: 5523063]
[5]
Stoddart MJ, Bara J, Alini M. Cells and secretome--towards endogenous cell re-activation for cartilage repair. Adv Drug Deliv Rev 2015; 84: 135-45.
[http://dx.doi.org/10.1016/j.addr.2014.08.007] [PMID: 25174306]
[6]
Allen MJ, Houlton JF, Adams SB, Rushton N. The surgical anatomy of the stifle joint in sheep. Vet Surg 1998; 27(6): 596-605.
[http://dx.doi.org/10.1111/j.1532-950X.1998.tb00536.x] [PMID: 9845224]
[7]
Goebel L, Orth P, Müller A, et al. Experimental scoring systems for macroscopic articular cartilage repair correlate with the MOCART score assessed by a high-field MRI at 9.4 T – comparative evaluation of five macroscopic scoring systems in a large animal cartilage defect model. Osteoarthritis Cartilage 2012; 20(9): 1046-55.
[http://dx.doi.org/10.1016/j.joca.2012.05.010] [PMID: 22698442]
[8]
Gruber HE, Ingram JA. Basic Staining Techniques for Cartilage Sections.In: An YH, Martin KL, Eds Handbook of Histology for Bone and Cartilage. United States: Humana Press 2003; pp. 287-93.
[9]
Pineda S, Pollack A, Stevenson S, Goldberg V, Caplan A. A semiquantitative scale for histologic grading of articular cartilage repair. Cells Tissues Organs 1992; 143(4): 335-40.
[http://dx.doi.org/10.1159/000147272] [PMID: 1502876]
[10]
Curl WW, Krome J, Gordon ES, Rushing J, Smith BP, Poehling GG. Cartilage injuries: A review of 31,516 knee arthroscopies. Arthroscopy 1997; 13(4): 456-60.
[http://dx.doi.org/10.1016/S0749-8063(97)90124-9] [PMID: 9276052]
[11]
Widuchowski W, Widuchowski J, Trzaska T. Articular cartilage defects: Study of 25,124 knee arthroscopies. Knee 2007; 14(3): 177-82.
[http://dx.doi.org/10.1016/j.knee.2007.02.001] [PMID: 17428666]
[12]
Getgood A, Bhullar TPS, Rushton N. Current concepts in articular cartilage repair. Orthop Trauma 2009; 23(3): 189-200.
[http://dx.doi.org/10.1016/j.mporth.2009.05.002]
[13]
Mithoefer K, McAdams T, Williams RJ, Kreuz PC, Mandelbaum BR. Clinical efficacy of the microfracture technique for articular cartilage repair in the knee: An evidence-based systematic analysis. Am J Sports Med 2009; 37(10): 2053-63.
[http://dx.doi.org/10.1177/0363546508328414] [PMID: 19251676]
[14]
Steadman JR, Briggs KK, Rodrigo JJ, Kocher MS, Gill TJ, Rodkey WG. Outcomes of microfracture for traumatic chondral defects of the knee: Average 11-year follow-up. Arthroscopy 2003; 19(5): 477-84.
[http://dx.doi.org/10.1053/jars.2003.50112] [PMID: 12724676]
[15]
Li X, Duan L, Liang Y, Zhu W, Xiong J, Wang D. Human umbilical cord blood-derived mesenchymal stem cells contribute to chondrogenesis in coculture with chondrocytes. BioMed Res Int 2016; 2016: 3827057.
[http://dx.doi.org/10.1155/2016/3827057] [PMID: 27446948]
[16]
Yu DA, Han J, Kim BS. Stimulation of chondrogenic differentiation of mesenchymal stem cells. Int J Stem Cells 2012; 5(1): 16-22.
[http://dx.doi.org/10.15283/ijsc.2012.5.1.16] [PMID: 24298351]
[17]
Zheng P, Ju L, Jiang B, et al. Chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells by co-culture with rabbit chondrocytes. Mol Med Rep 2013; 8(4): 1169-74.
[http://dx.doi.org/10.3892/mmr.2013.1637] [PMID: 23969943]
[18]
Soetjahjo B. Hı̇dayat M, Sujutiı̇ H, Fı̇brı̇ anto YH. The significant effect of conditioned medium of umbilical cord mesenchymal stem cells in histological improvement of cartilage defect in wistar rats. Turk J Immunol 2018; 6(2): 57-64.
[http://dx.doi.org/10.25002/tji.2018.682]
[19]
Mak J, Jablonski CL, Leonard CA, et al. Intra-articular injection of synovial mesenchymal stem cells improves cartilage repair in a mouse injury model. Sci Rep 2016; 6(1): 23076.
[http://dx.doi.org/10.1038/srep23076] [PMID: 26983696]
[20]
Marquass B, Schulz R, Hepp P, et al. Matrix-associated implantation of predifferentiated mesenchymal stem cells versus articular chondrocytes: In vivo results of cartilage repair after 1 year. Am J Sports Med 2011; 39(7): 1401-12.
[http://dx.doi.org/10.1177/0363546511398646] [PMID: 21527412]
[21]
Mehrabani D, Babazadeh M, Tanideh N, et al. The healing effect of adipose-derived mesenchymal stem cells in full-thickness femoral articular cartilage defects of rabbit. Int J Organ Transplant Med 2015; 6(4): 165-75.
[PMID: 26576262]
[22]
Chung JY, Song M, Ha CW, Kim JA, Lee CH, Park YB. Comparison of articular cartilage repair with different hydrogel-human umbilical cord blood-derived mesenchymal stem cell composites in a rat model. Stem Cell Res Ther 2014; 5(2): 39.
[http://dx.doi.org/10.1186/scrt427] [PMID: 24646697]

Rights & Permissions Print Cite
Article Metrics
3
© 2024 Bentham Science Publishers | Privacy Policy