Login Register Cart 0
Generic placeholder image

Current Cancer Therapy Reviews

Editor-in-Chief

ISSN (Print): 1573-3947
ISSN (Online): 1875-6301

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Assessment of Expression of LncRNA H19, Pinin and their Relation to rs532 Single Nucleotide Polymorphism in the Pinin Gene Present in the Tissue of Individuals Suffering from Breast Cancer in Central Iran

Author(s): Fariba Nabatchian*, Zahra Shafieizadeh, Zohreh Shafieizadeh*, Afshin Moradi, Fatemeh Kheiri, Mansoureh Azadeh, Reza Afrisham and Negin Davoudi

Volume 18, Issue 2, 2022

Published on: 28 June, 2022

Page: [143 - 147] Pages: 5

DOI: 10.2174/1573394718666220413124759

Price: $65

Abstract

Introduction: In women, breast cancer is a prevalent malignancy. One of the important genes in breast cancer progression is Pinin. LncRNAs H19 plays an important role in breast cancer cell development. In this study, the correlation between a miRNA-related rs532 polymorphism (G) and the level of expression of Pinin and lncRNA H19 in breast cancer tissues was investigated.

Methods: Tissue samples were collected from 64 patients with breast cancer. RT-PCR with highresolution melting (HRM) analysis was used.

Results: Our data suggest that there is significant up-regulation in the expression of lncRNA H19 and the Pinin gene in breast cancer tissue compared to the control ones. The Pinin polymorphism rs532 G is significantly associated with Pinin expression of breast cancer tissue compared to the control one.

Discussion: The rs532 G polymorphism is located around the pinin and its allele frequency is significantly associated with Pinin expression. Perhaps, rs532 G polymorphism could be functional in breast cancer progression through the impact on pinin expression. There is no correlation between SNP and lncRNA H19.

Conclusion: The polymorphism rs532 G polymorphism is significantly associated with Pinin expression of breast cancer tissue from Isfahan patients which suggests the miRNA-141has potential to regulate Pinin expression levels.

Keywords: Breast cancer, polymorphism, Pinin gene, LncRNA H19 gene, rs532, tumor.

« Previous Next »
Graphical Abstract

[1]
Zabihi N, Sadeghi S, Tabatabaeian H, Ghaedi K, Azadeh M, Fazilati M. The association between rs1972820 and the risk of breast cancer in Isfahan population. J Cancer Res Ther 2017; 13(1): 26-32.
[http://dx.doi.org/10.4103/0973-1482.183202] [PMID: 28508829]
[2]
Shi Y, Ouyang P, Sugrue SP. Characterization of the gene encoding pinin/DRS/memA and evidence for its potential tumor suppressor function. Oncogene 2000; 19(2): 289-97.
[http://dx.doi.org/10.1038/sj.onc.1203328] [PMID: 10645008]
[3]
Yang X, Sun D, Dong C, Tian Y, Gao Z, Wang L. Pinin associates with prognosis of hepatocellular carcinoma through promoting cell pro-liferation and suppressing glucose deprivation-induced apoptosis. Oncotarget 2016; 7(26): 39694-704.
[http://dx.doi.org/10.18632/oncotarget.9233] [PMID: 27175589]
[4]
Collette J, Le Bourhis X, Adriaenssens E. Regulation of human breast cancer by the long non-coding rna H19. Int J Mol Sci 2017; 18(11): E2319.
[http://dx.doi.org/10.3390/ijms18112319] [PMID: 29099749]
[5]
Nagarajan A, Malvi P, Wajapeyee N. Oncogene-directed alterations in cancer cell metabolism. Trends Cancer 2016; 2(7): 365-77.
[http://dx.doi.org/10.1016/j.trecan.2016.06.002] [PMID: 27822561]
[6]
Abbastabar M, Sarfi M, Golestani A, Khalili E. lncRNA involvement in hepatocellular carcinoma metastasis and prognosis. EXCLI J 2018; 17: 900-13.
[PMID: 30564069]
[7]
Liu C, Chen Z, Fang J, Xu A, Zhang W, Wang Z. H19-derived miR-675 contributes to bladder cancer cell proliferation by regulating p53 activation. Tumour Biol 2016; 37(1): 263-70.
[http://dx.doi.org/10.1007/s13277-015-3779-2] [PMID: 26198047]
[8]
Dai L, Li J, Dong Z, et al. Temporal expression and functional analysis of long non-coding RNAs in colorectal cancer initiation. J Cell Mol Med 2019; 23(6): 4127-38.
[http://dx.doi.org/10.1111/jcmm.14300] [PMID: 30920116]
[9]
Zhu Z, Song L, He J, Sun Y, Liu X, Zou X. Ectopic expressed long non-coding RNA H19 contributes to malignant cell behavior of ovarian cancer. Int J Clin Exp Pathol 2015; 8(9): 10082-91.
[PMID: 26617715]
[10]
Gao H, Hao G, Sun Y, Li L, Wang Y. Long noncoding RNA H19 mediated the chemosensitivity of breast cancer cells via Wnt pathway and EMT process. OncoTargets Ther 2018; 11: 8001-12.
[http://dx.doi.org/10.2147/OTT.S172379] [PMID: 30519041]
[11]
He P, Zhang Z, Huang G, et al. miR-141 modulates osteoblastic cell proliferation by regulating the target gene of lncRNA H19 and lncRNA H19-derived miR-675. Am J Transl Res 2016; 8(4): 1780-8.
[PMID: 27186302]
[12]
Lee DY, Jeyapalan Z, Fang L, et al. Expression of versican 3′-untranslated region modulates endogenous microRNA functions. PLoS One 2010; 5(10): e13599.
[http://dx.doi.org/10.1371/journal.pone.0013599] [PMID: 21049042]
[13]
Matouk IJ, Halle D, Raveh E, Gilon M, Sorin V, Hochberg A. The role of the oncofetal H19 lncRNA in tumor metastasis: Orchestrating the EMT-MET decision. Oncotarget 2016; 7(4): 3748-65.
[http://dx.doi.org/10.18632/oncotarget.6387] [PMID: 26623562]
[14]
Zhou X, Ye F, Yin C, Zhuang Y, Yue G, Zhang G. The interaction between MiR-141 and lncRNA-H19 in regulating cell proliferation and migration in gastric cancer. Cell Physiol Biochem 2015; 36(4): 1440-52.
[http://dx.doi.org/10.1159/000430309] [PMID: 26160158]
[15]
Meng L, Ma P, Cai R, Guan Q, Wang M, Jin B. Long noncoding RNA ZEB1-AS1 promotes the tumorigenesis of glioma cancer cells by modulating the miR-200c/141-ZEB1 axis. Am J Transl Res 2018; 10(11): 3395-412.
[PMID: 30662595]
[16]
Felekkis K, Touvana E, Stefanou Ch, Deltas C. microRNAs: A newly described class of encoded molecules that play a role in health and disease. Hippokratia 2010; 14(4): 236-40.
[PMID: 21311629]

Rights & Permissions Print Cite
Article Metrics
12
1
© 2024 Bentham Science Publishers | Privacy Policy