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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

Pharmacokinetics Study of Rabdosia rubescens Drop Pills Based on UPLC-MS/MS

Author(s): Xi-yu Wei, Zhen-zhen Zhang, Tao Xie, Yue-Sheng Xie, Yue Sun, Ting Yang, Wen-wu Xu, Hou-ru Liu, Wei Li* and De-hong Yu*

Volume 18, Issue 5, 2022

Published on: 24 January, 2022

Page: [491 - 503] Pages: 13

DOI: 10.2174/1573412918666211230095348

Price: $65

Abstract

Background: Rabdosia rubescens drop pills have the effects of clearing away heat and toxin, detumescence, relieving pain.

Objective: A simple and sensitive method for simultaneous determination of oridonin, ponicidin, and rosmarinic acid in rat plasma was developed based on ultra performance liquid chromatography- tandem mass spectrometry (UPLC-MS/MS).

Methods: Chromatographic separation was performed on a Waters ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a mobile phase consisting of water containing 0.2% formic acid (mobile phase A) and methanol (mobile phase B) at a flow rate of 0.3 mL/min over a total run time of 3.8 min. All analytes were measured with optimized multiple reaction monitoring (MRM) in positive and negative ion ESI mode.

Results: The transitions of oridonin, ponicidin, rosmarinic acid, diphenhydramine, and chloramphenicol were 365.3→347.3, 363.3→345.2, 359.0→160.9, 256.0→167.2, and 321.1→151.9, respectively. The linear ranges were 1-256 ng/mL for ponicidin and rosmarinic acid and 2-512 ng/mL for oridonin. The validated method was stable and reliable. There was no significant difference in the half-life (t1/2) of the three analytes at three doses. The area under the curve (AUC0-t) and peak concentration (Cmax) of the three analytes decreased linearly in each dose range, and the linear correlation R2 of each analyte under the three doses was greater than 0.95.

Conclusion: This method was successfully applied to pharmacokinetic studies of oridonin, ponicidin, and rosmarinic acid in rat plasma after intragastric administration of Rabdosia rubescens drop pills.

Keywords: Oridonin, pharmacokinetics, ponicidin, rosmarinic acid, UPLC-MS/MS, Rabdosia rubescens.

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Graphical Abstract

[1]
Yang, Y.C.; Wei, M.C.; Huang, T.C. Optimisation of an ultrasound-assisted extraction followed by RP-HPLC separation for the simultaneous determination of oleanolic acid, ursolic acid and oridonin content in Rabdosia rubescens. Phytochem. Anal., 2012, 23(6), 627-636.
[http://dx.doi.org/10.1002/pca.2365] [PMID: 22706975]
[2]
Lu, Y.; Sun, C.; Liu, R.; Pan, Y. Effective two-dimensional counter-current chromatographic method for simultaneous isolation and purification of oridonin and ponicidin from the crude extract of Rabdosia rubescens. J. Chromatogr. A, 2007, 1146(1), 125-130.
[http://dx.doi.org/10.1016/j.chroma.2007.01.141] [PMID: 17328904]
[3]
He, H.; Jiang, H.; Chen, Y.; Ye, J.; Wang, A.; Wang, C.; Liu, Q.; Liang, G.; Deng, X.; Jiang, W.; Zhou, R. Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity. Nat. Commun., 2018, 9(1), 2550.
[http://dx.doi.org/10.1038/s41467-018-04947-6] [PMID: 29959312]
[4]
Kou, B.; Yang, Y.; Bai, Y.E.; Shi, Y.H.; Gao, R.X.; Yang, F.L.; Zhang, S.Q.; Liu, W. Oridonin induces apoptosis of laryngeal carcinoma via endoplasmic reticulum stress. Cancer Manag. Res., 2020, 12, 8387-8396.
[http://dx.doi.org/10.2147/CMAR.S271759] [PMID: 32982432]
[5]
Yan, Y.; Tan, R.Z.; Liu, P.; Li, J.C.; Zhong, X.; Liao, Y.; Lin, X.; Wei, C.; Wang, L. Oridonin alleviates IRI-induced kidney injury by inhibiting inflammatory response of macrophages via AKT-related pathways. Med. Sci. Monit., 2020, 26e921114
[http://dx.doi.org/10.12659/MSM.921114] [PMID: 32362652]
[6]
Han, J.M.; Hong, K.O.; Yang, I.H.; Ahn, C.H.; Jin, B.; Lee, W.; Jung, Y.C.; Kim, K.A.; Shin, J.A.; Cho, S.D.; Hong, S.D. Oridonin induces the apoptosis of mucoepidermoid carcinoma cell lines in a myeloid cell leukemia 1 dependent manner. Int. J. Oncol., 2020, 57(1), 377-385.
[http://dx.doi.org/10.3892/ijo.2020.5061] [PMID: 32467983]
[7]
Islam, M.T.; Biswas, S.; Bagchi, R.; Khan, M.R.; Khalipha, A.B.R.; Rouf, R.; Uddin, S.J.; Shilpi, J.A.; Bardaweel, S.K.; Sabbah, D.A.; Mubarak, M.S. Ponicidin as a promising anticancer agent: Its biological and biopharmaceutical profile along with a molecular docking study. Biotechnol. Appl. Biochem., 2019, 66(3), 434-444.
[http://dx.doi.org/10.1002/bab.1740] [PMID: 30801842]
[8]
Liu, Y.F.; Lu, Y.M.; Qu, G.Q.; Liu, Y.; Chen, W.X.; Liao, X.H.; Kong, W.M. Ponicidin induces apoptosis via JAK2 and STAT3 signaling pathways in gastric carcinoma. Int. J. Mol. Sci., 2015, 16(1), 1576-1589.
[http://dx.doi.org/10.3390/ijms16011576] [PMID: 25588213]
[9]
Zhang, Z.; Xu, J.; Liu, B.; Chen, F.; Li, J.; Liu, Y.; Zhu, J.; Shen, C. Ponicidin inhibits pro-inflammatory cytokine TNF-α-induced epithelial-mesenchymal transition and metastasis of colorectal cancer cells via suppressing the AKT/GSK-3β/Snail pathway. Inflammopharmacology, 2019, 27(3), 627-638.
[http://dx.doi.org/10.1007/s10787-018-0534-5] [PMID: 30244296]
[10]
Wei, Y.; Chen, J.; Hu, Y.; Lu, W.; Zhang, X.; Wang, R.; Chu, K. Rosmarinic acid mitigates lipopolysaccharide-induced neuroinflammatory responses through the inhibition of TLR4 and CD14 expression and NF-κB and NLRP3 inflammasome activation. Inflammation, 2018, 41(2), 732-740.
[http://dx.doi.org/10.1007/s10753-017-0728-9] [PMID: 29318480]
[11]
Mei, Y.; Xu, J.; Zhao, J.; Feng, N.; Liu, Y.; Wei, L. An HPLC method for determination of oridonin in rabbits using isopsoralen as an internal standard and its application to pharmacokinetic studies for oridonin-loaded nanoparticles. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2008, 869(1-2), 138-141.
[http://dx.doi.org/10.1016/j.jchromb.2008.05.005] [PMID: 18514594]
[12]
Ge, J.; You-Wei, W.; Xiao-Cong, L.; Ji-Yue, C. Determination of oridonin in rat plasma by reverse-phase high-performance liquid chromatography. J. Pharm. Biomed. Anal., 2007, 43(2), 793-797.
[http://dx.doi.org/10.1016/j.jpba.2006.08.016] [PMID: 17011736]
[13]
Li, X.; Hou, W.; Song, J.; Liu, H.; Song, S.; Zhang, L.; Shi, Y. A simple and sensitive HPLC-UV method for the determination of ponicidin in rat plasma and its application in a pharmacokinetic study. Biomed. Chromatogr., 2011, 25(3), 362-366.
[http://dx.doi.org/10.1002/bmc.1456] [PMID: 21110387]
[14]
Li, X.; Chu, Y.; Du, B.; Wang, L.; Yu, T. LC-MS-MS for the determination of ponicidin in dog plasma. J. Chromatogr. Sci., 2014, 52(3), 211-217.
[http://dx.doi.org/10.1093/chromsci/bmt013] [PMID: 23505290]
[15]
Xu, W.; Sun, J.; Zhang, T.T.; Ma, B.; Cui, S.M.; Chen, D.W.; He, Z.G. Pharmacokinetic behaviors and oral bioavailability of oridonin in rat plasma. Acta Pharmacol. Sin., 2006, 27(12), 1642-1646.
[http://dx.doi.org/10.1111/j.1745-7254.2006.00440.x] [PMID: 17112421]
[16]
Liu, Y.; Li, X.; Li, Y.; Wang, L.; Xue, M. Simultaneous determination of danshensu, rosmarinic acid, cryptotanshinone, tanshinone IIA, tanshinone I and dihydrotanshinone I by liquid chromatographic-mass spectrometry and the application to pharmacokinetics in rats. J. Pharm. Biomed. Anal., 2010, 53(3), 698-704.
[http://dx.doi.org/10.1016/j.jpba.2010.03.041] [PMID: 20430561]
[17]
Du, Y.; Liu, P.; Shi, X.; Jin, Y.; Wang, Q.; Zhang, X.; Sheng, X.; Zhang, L. A novel analysis method for diterpenoids in rat plasma by liquid chromatography-electrospray ionization mass spectrometry. Anal. Biochem., 2010, 407(1), 111-119.
[http://dx.doi.org/10.1016/j.ab.2010.07.009] [PMID: 20643094]
[18]
Ma, B.; Wang, Y.; Zhang, Q.; Liu, Y.; Li, J.; Xu, Q.; Ying, H. Simultaneous determination of oridonin, ponicidin and rosmarinic acid from herba Isodi rubescentis extract by LC-MS-MS in rat plasma. J. Chromatogr. Sci., 2013, 51(10), 910-918.
[http://dx.doi.org/10.1093/chromsci/bms189] [PMID: 23357046]
[19]
Chen, S.; Liu, J.; Zhang, H. Efficacy of Rabdosia rubescens in the treatment of gingivitis. J. Huazhong Univ. Sci. Technolog. Med. Sci., 2009, 29(5), 659-663.
[http://dx.doi.org/10.1007/s11596-009-0525-2] [PMID: 19821105]
[20]
Kang, N.; Cao, S.; Jiang, B.; Zhang, Q.; Donkor, P.O.; Zhu, Y.; Qiu, F.; Gao, X. Cetuximab enhances oridonin-induced apoptosis through mitochondrial pathway and endoplasmic reticulum stress in laryngeal squamous cell carcinoma cells. Toxicol. In Vitro, 2020, 67104885
[http://dx.doi.org/10.1016/j.tiv.2020.104885] [PMID: 32407876]
[21]
Jiang, J.H.; Pi, J.; Jin, H.; Cai, J.Y. Oridonin-induced mitochondria-dependent apoptosis in esophageal cancer cells by inhibiting PI3K/AKT/mTOR and Ras/Raf pathways. J. Cell. Biochem., 2019, 120(3), 3736-3746.
[http://dx.doi.org/10.1002/jcb.27654] [PMID: 17112421]

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