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Research Article

丁酸钠预处理增强骨髓间充质干细胞（BM-MSCs）向肝细胞的分化并改善肝损伤

卷 22, 期 7, 2022

发表于: 06 January, 2022

页: [663 - 674] 页: 12

弟呕挨: 10.2174/1566524021666211014161716

价格: $65

摘要

目的：干细胞移植治疗肝功能衰竭引起了越来越多的关注。在此，我们旨在探索丁酸钠（NaB）在体外和体内肝脏特异性因子诱导下骨髓间充质干细胞（BM-MSCs）肝脏分化中的作用。 材料与方法：我们从兔骨髓样品的单核细胞部分中分离出 BM-MSCs，并通过免疫表型分析鉴定细胞。我们研究了不同浓度和诱导条件的影响。组蛋白去乙酰化酶抑制剂 NaB 在体外肝特异性因子诱导下诱导 BM-MSCs 肝分化。进行形态学特征、肝脏特异性基因和蛋白质表达以及体外和体内功能分析以评估BM-MSCs的肝脏分化。 结果：我们的结果表明，预处理的 NaB 以剂量依赖性方式抑制肝脏特异性蛋白的表达。 NaB预处理24h诱导效率高于NaB连续干预。 0.5 mM 24h NaB 预处理细胞可改善体内肝组织损伤。肝脏ALB、AAT和血清TP明显升高，而血清ALT明显降低。 结论：连续NaB处理在一定浓度范围内能以剂量依赖性方式抑制BM-MSCs增殖。 0.5 mM NaB 预处理 24 小时可增强 BM-MSCs 向肝细胞的分化，并改善体外和体内的肝损伤。

关键词: 骨髓间充质干细胞，丁酸钠，肝分化，肝损伤，体外，体内。

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