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Research Article

新设计的脱细胞支架，用于通过超临界二氧化碳流体和碱性/蛋白酶处理从弹性软骨进行基于支架的基因治疗

卷 22, 期 2, 2022

发表于: 18 June, 2021

页: [162 - 167] 页: 6

弟呕挨: 10.2174/1566523219666210618151843

价格: $65

摘要

背景：基于支架的基因治疗为组织工程提供了一种有前途的方法，它结合了医学应用和工程材料的知识，因此非常重要和流行。 目的：采用脱细胞技术从猪弹性软骨中去除细胞成分，留下天然脱细胞细胞外基质 (dECM) 组合物和大部分不溶性胶原蛋白、弹性蛋白和紧密结合的糖胺聚糖的结构完整性。对于新设计的胶原支架样品，弹性软骨被不同浓度的蛋白酶水解，获得完整清晰的状态。 方法：采用超临界二氧化碳（ScCO2）提取工艺从猪弹性软骨中去除细胞成分。具有胶原蛋白的 dECM 支架必须通过傅里叶变换红外光谱 (FTIR)、热重分析 (TGA) 和扫描电子显微镜 (SEM) 进行表征。 结果：该研究提供了一种结合超临界二氧化碳和碱性/蛋白酶的方法制备具有孔-支架微结构的dECM支架，并引入了基于支架的基因治疗在骨软骨组织工程中的潜在应用。新工艺简单高效。在源自猪弹性软骨的 dECM 支架中观察到孔支架微结构。在超过 330oC 时观察到所得 dECM 支架的 Tdmax 值。 结论：采用 ScCO2 和碱/酶处理（如 NH4OH 和木瓜蛋白酶的混合水溶液）成功地从猪组织中获得了一系列新型支架。获得了具有高热稳定性的dECM支架。由此产生的具有清洁孔支架微结构的支架可能是基于支架的基因治疗的潜在应用。

关键词: 蛋白酶、木瓜蛋白酶、超临界二氧化碳、弹性软骨、dECM、基于支架的基因治疗

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