Login Register Cart 0
Generic placeholder image

Current Proteomics

Editor-in-Chief

ISSN (Print): 1570-1646
ISSN (Online): 1875-6247

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Evaluation of the Binding Affinity of a Gonadotropin-Releasing Hormone Analogue (GnRH-a) Buserelin through In silico and In vivo Testing in Clarias magur

Author(s): Mukesh Kumar, Mukunda Goswami*, Sunil Kumar Nayak , P. Gireesh-Babu and Aparna Chaudhari

Volume 19, Issue 2, 2022

Published on: 26 April, 2021

Page: [163 - 170] Pages: 8

DOI: 10.2174/1570164618666210426090916

Price: $65

Abstract

Aim: To evaluate the binding affinity and biological potency of gonadotropin-releasing hormone analogue (GnRHa) Buserelin (C60H86N16O13) based on in silico and in vivo testing for induced breeding in Clarias magur.

Background: Many attempts have been made to induce C. magur, but encouraging results have not yet been achieved. Hence, it is the need of the hour to find out more potent analogues or other bio-molecules for induced breeding in C. magur to facilitate sustainable aquaculture.

Objective: To determine the binding affinity of C. magur GnRH receptor through in silico and to validate it for induced breeding of C. magur.

Methods: Buserelin (C60H86N16O13) was selected as the potential GnRHa after screening several peptides for their binding energy with the C. magur GnRH receptor. The induced breeding trial was set up at ICAR-CIFE Powarkheda Centre, M.P. India, and Buserelin was administered in different doses to the brooders along with the dopamine inhibitor domperidone. The standard treatment with the commercial salmon GnRH (sGnRH) analogue Ovaprim® (Syndel, USA) was used as the control.

Results: The 3-D structure of C. magur GnRH receptor was generated using MODELLER software. Molecular docking studies revealed the binding preference of the receptor as chicken (c) Gn- RH-II > Buserelin > sGnRH > catfish (cf) GnRH > human (m) GnRH. Though Buserelin showed better binding affinity compared to sGnRH, induced breeding experiments with magur showed similar performance of the ligands at the equivalent dose of 20 μg/kg B.W., but the spontaneous release of milt from the males was not observed in both cases. Significantly better reproductive parameters were recorded with Buserelin at the dose of 30 μg/kg B.W.

Conclusion: The study revealed that that the GnRHa Buserelin can be used as an effective inducing agent for breeding in C. magur.

Keywords: Freshwater catfish, bioinformatics, protein modelling, ligand–receptor interaction, Clarias magur, buserelin.

« Previous Next »
Graphical Abstract

[1]
Clarias batrachus, the medicinal fish: An excellent candidate for aquaculture & employment generation. Debnath, S., Ed.; International Conference on Asia Agriculture and Animal IPCBEE, Singapore 2011, 13
[2]
Kumar, M.; Kumar, S.; Acharya, A.P.; Agarwal, D.; Laishram, M.; Das, R. Effect of different probe hybridization methods on the yield of simple sequence repeat loci in clarias batrachus. Int J Pure App Biosci., 2017, 5(4), 2081-2089.
[http://dx.doi.org/10.18782/2320-7051.5252]
[3]
Talukdar, A.; Kumar, S.; Varghese, T.; Jain, K.K.; Sahu, N.P.; Sahoo, S. Feeding gelatinized carbohydrate in the diets of magur, Clarias batrachus (Linnaeus, 1758): Effects on growth performance, enzyme activities and expression of muscle regulatory factors. Aquacult. Res., 2019, 50(3), 765-777.
[4]
Majhi, S.K.; Maurya, P.K.; Kumar, S.; Mohindra, V.; Lal, K.K. Non-invasive method for collection of clarias magur spermatozoa for breeding. MethodsX, 2020, 7, 100929.
[http://dx.doi.org/10.1016/j.mex.2020.100929] [PMID: 32566489]
[5]
Srivastava, P.P.; Raizada, S.; Dayal, R.; Chowdhary, S.; Lakra, W.S.; Yadav, A.K. Breeding and larval rearing of asian catfish, clarias batrachus (linnaeus, 1758) on live and artificial feed. J. Aquac. Res. Dev., 2012, 3(4)
[http://dx.doi.org/10.4172/2155-9546.1000134]
[6]
Sinha, M; Mahapatra, B; Saha, D; Maitra, N Mass scale seed production of Magur, Clarias batrachus at farm level through improvised modifications. Int. j. fish., 2014, 2(2), 210-214.
[7]
Millar, R.P.; Lu, Z-L.; Pawson, A.J.; Flanagan, C.A.; Morgan, K.; Maudsley, S.R. Gonadotropin-releasing hormone receptors. Endocr. Rev., 2004, 25(2), 235-275.
[http://dx.doi.org/10.1210/er.2003-0002] [PMID: 15082521]
[8]
Sahoo, S.; Giri, S.; Chandra, S.; Sahu, A. Spawning performance and egg quality of Asian catfish Clarias batrachus (Linn.) at various doses of human chorionic gonadotropin (HCG) injection and latency periods during spawning induction. Aquaculture, 2007, 266(1-4), 289-292.
[http://dx.doi.org/10.1016/j.aquaculture.2007.02.006]
[9]
Dhandare, B.C.; Rather, M.A.; Bhosale, B.P.; Pawar, R.; Guttula, P.K.; Pagarkar, A.U. Molecular modeling, docking and dynamic simulations of growth hormone receptor (GHR) of Labeo rohita. J. Biomol. Struct. Dyn., 2020, 1-14.
[http://dx.doi.org/10.1080/07391102.2020.1844063] [PMID: 33179589]
[10]
Schneider, F.; Tomek, W.; Gründker, C. Gonadotropin-releasing hormone (GnRH) and its natural analogues: A review. Theriogenology, 2006, 66(4), 691-709.
[http://dx.doi.org/10.1016/j.theriogenology.2006.03.025] [PMID: 16650469]
[11]
Okubo, K.; Nagahama, Y. Structural and functional evolution of gonadotropin-releasing hormone in vertebrates. Acta Physiol. (Oxf.), 2008, 193(1), 3-15.
[http://dx.doi.org/10.1111/j.1748-1716.2008.01832.x] [PMID: 18284378]
[12]
Lethimonier, C.; Madigou, T.; Muñoz-Cueto, J-A.; Lareyre, J-J.; Kah, O. Evolutionary aspects of GnRHs, GnRH neuronal systems and GnRH receptors in teleost fish. Gen. Comp. Endocrinol., 2004, 135(1), 1-16.
[http://dx.doi.org/10.1016/j.ygcen.2003.10.007] [PMID: 14644639]
[13]
Grosse, R.; Schmid, A.; Schöneberg, T.; Herrlich, A.; Muhn, P.; Schultz, G.; Gudermann, T. Gonadotropin-releasing hormone receptor initiates multiple signaling pathways by exclusively coupling to G(q/11) proteins. J. Biol. Chem., 2000, 275(13), 9193-9200.
[http://dx.doi.org/10.1074/jbc.275.13.9193] [PMID: 10734055]
[14]
Parker, K.; Schimmer, B. Pituitary hormones and their hypothalamic releasing factors.Goodman & Gilman’s The Pharmacological Basis of Therapeutics New York; McGraw-Hill Medical Publishing Division: NY, 2001, pp. 1541-1562.
[15]
Yano, T.; Yano, N.; Matsumi, H.; Morita, Y.; Tsutsumi, O.; Schally, A.V.; Taketani, Y. Effect of luteinizing hormone-releasing hormone analogs on the rat ovarian follicle development. Horm. Res., 1997, 48(Suppl. 3), 35-41.
[http://dx.doi.org/10.1159/000191298] [PMID: 9267814]
[16]
Saltan, G.; Süntar, I.; Ozbilgin, S.; Ilhan, M.; Demirel, M.A.; Oz, B.E.; Keleş, H.; Akkol, E.K. Viburnum opulus L.: A remedy for the treatment of endometriosis demonstrated by rat model of surgically-induced endometriosis. J. Ethnopharmacol., 2016, 193, 450-455.
[http://dx.doi.org/10.1016/j.jep.2016.09.029] [PMID: 27647013]
[17]
Bryant, A.S.; Greenwood, A.K.; Juntti, S.A.; Byrne, A.E.; Fernald, R.D. Dopaminergic inhibition of gonadotropin-releasing hormone neurons in the cichlid fish Astatotilapia burtoni. J. Exp. Biol., 2016, 219(Pt 24), 3861-3865.
[http://dx.doi.org/10.1242/jeb.147637] [PMID: 27742893]
[18]
Bardal, SK; Waechter, JE; Martin, DS Applied pharmacology: elsevier health sciences. 2011.
[19]
Agarwal, D.; Gireesh-Babu, P.; Pavan-Kumar, A.; Koringa, P.; Joshi, C.G.; Chaudhari, A. Transcriptome analysis of Clarias magur brain and gonads suggests neuro-endocrine inhibition of milt release from captive GnRH-induced males. Genomics, 2020, 112(6), 4041-4052.
[http://dx.doi.org/10.1016/j.ygeno.2020.07.012] [PMID: 32650102]
[20]
Schulz, R.W.; Bosma, P.T.; Zandbergen, M.A.; Van der Sanden, M.C.; Van Dijk, W.; Peute, J.; Bogerd, J.; Goos, H.J. Two gonadotropin-releasing hormones in the African catfish, Clarias gariepinus: Localization, pituitary receptor binding, and gonadotropin release activity. Endocrinology, 1993, 133(4), 1569-1577.
[http://dx.doi.org/10.1210/endo.133.4.8404596] [PMID: 8404596]
[21]
Ramachandran, G.N.; Ramakrishnan, C.; Sasisekharan, V. Stereochemistry of polypeptide chain configurations. J. Mol. Biol., 1963, 7, 95-99.
[http://dx.doi.org/10.1016/S0022-2836(63)80023-6] [PMID: 13990617]
[22]
Spencer, R.K.; Butterfoss, G.L.; Edison, J.R.; Eastwood, J.R.; Whitelam, S.; Kirshenbaum, K.; Zuckermann, R.N. Stereochemistry of polypeptoid chain configurations. Biopolymers, 2019, 110(6), e23266.
[http://dx.doi.org/10.1002/bip.23266] [PMID: 30835823]
[23]
Wiederstein, M; Sippl, MJ ProSA-web: Interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Res., 2007, 35(suppl_2), W407-W410.
[http://dx.doi.org/10.1093/nar/gkm290]
[24]
Eisenberg, D; Lüthy, R; Bowie, J. VERIFY3D: Assessment of protein models with three-dimensional profiles. Methods in enzymology, 1997, Vol. 277, 396-404.
[25]
Krebs, B.B.; De Mesquita, J.F. Amyotrophic lateral sclerosis type 20-In Silico analysis and molecular dynamics simulation of hnRNPA. PLoS One, 2016, 11(7), e0158939.
[http://dx.doi.org/10.1371/journal.pone.0158939] [PMID: 27414033]
[26]
de Vries, S.J.; van Dijk, M.; Bonvin, A.M. The HADDOCK web server for data-driven biomolecular docking. Nat. Protoc., 2010, 5(5), 883-897.
[http://dx.doi.org/10.1038/nprot.2010.32] [PMID: 20431534]
[27]
Thalathiah, S.; Fauzi, O.A.; Ibrahim, T. First Successful Attempt to Induce Breed Mystus Nemurus (C & V) at Batu Berendam Melaka; Malaysian Society of Animal Production, 1988.
[28]
El-Hawarry, W.N.; Abd El-Rahman, S.H.; Shourbela, R.M. Breeding response and larval quality of African catfish (Clarias gariepinus, Burchell 1822) using different hormones/hormonal analogues with dopamine antagonist. Egypt. J. Aquat. Res., 2016, 42(2), 231-239.
[http://dx.doi.org/10.1016/j.ejar.2016.06.003]
[29]
Thomas, P. Breeding and seed production of fin fish and shell fish: Daya Books. 2003.
[30]
Adebayo, O. Reproductive performance of African Clariid Catfish Clarias gariepinus broodstock on varying maternal stress. Journal of Fisheries international, 2006, 1(1-2), 17-20.
[31]
Moran, LA; Horton, RA; Scrimgeour, KG; Perry, MD Principles of biochemistry. 2014.
[32]
Wiltgen, M. Algorithms for structure comparison and analysis: Homology modelling of proteins.Encyclopedia of Bioinformatics and Computational Biology; Ranganathan, S.; Gribskov, M.; Nakai, K.; Schönbach, C., Eds.; , 2018, Vol. 21, pp. 38-61.
[33]
Dominguez, C.; Boelens, R.; Bonvin, A.M. HADDOCK: A protein-protein docking approach based on biochemical or biophysical information. J. Am. Chem. Soc., 2003, 125(7), 1731-1737.
[http://dx.doi.org/10.1021/ja026939x] [PMID: 12580598]
[34]
Flanagan, C.A.; Manilall, A. Gonadotropin-releasing hormone (GnRH) receptor structure and GnRH binding. Front. Endocrinol. (Lausanne), 2017, 8, 274.
[http://dx.doi.org/10.3389/fendo.2017.00274] [PMID: 29123501]
[35]
Cheng, C.K.; Leung, P.C. Molecular biology of gonadotropin-releasing hormone (GnRH)-I, GnRH-II, and their receptors in humans. Endocr. Rev., 2005, 26(2), 283-306.
[http://dx.doi.org/10.1210/er.2003-0039] [PMID: 15561800]
[36]
Bogerd, J.; Diepenbroek, W.B.; Hund, E.; van Oosterhout, F.; Teves, A.C.; Leurs, R.; Blomenröhr, M. Two gonadotropin-releasing hormone receptors in the African catfish: no differences in ligand selectivity, but differences in tissue distribution. Endocrinology, 2002, 143(12), 4673-4682.
[http://dx.doi.org/10.1210/en.2002-220578] [PMID: 12446595]
[37]
Brogden, R.N.; Buckley, M.M-T.; Ward, A. Buserelin. A review of its pharmacodynamic and pharmacokinetic properties, and clinical profile. Drugs, 1990, 39(3), 399-437.
[http://dx.doi.org/10.2165/00003495-199039030-00007] [PMID: 2109679]
[38]
Hazum, E.; Conn, P.M. Molecular mechanism of gonadotropin releasing hormone (GnRH) action. I. The GnRH receptor. Endocr. Rev., 1988, 9(4), 379-386.
[http://dx.doi.org/10.1210/edrv-9-4-379] [PMID: 2851440]
[39]
Basavaraja, N.; Nandeesha, M.; Akash, N.; Rangaswamy, B.; Mahesh, T.; Murthy, C. Successful induced spawning of Indian major carps following injection of buserelin and domperidone. Asian Fish. Sci., 2007, 20(3/4), 287.
[40]
Taslima, K.; Ahmed, F. Study on seed production technique of indigenous magur (Clarias batrachus), shing (Heteropneustes fossilis) and pabda (Ompok pabda) through induced breeding. BEPLS, 2012, 1(4), 16-23.
[41]
Sahoo, S.; Ferosekhan, S.; Giri, S.; Swain, S. Recent trends in breeding and seed production of Magur in India. World Aquaculture Magazine., 2016, 47(2), 59-62.
[42]
Dhara, K.; Saha, N.C. Controlled breeding of asian catfish clarias batrachus using pituitary gland extracts and ovaprim at different temperatures, latency periods and their early development. J. Aquac. Res. Dev., 2013, 4(4)
[43]
Niveshika, ; Verma, E.; Maurya, S.K.; Mishra, R.; Mishra, A.K. The combined use of in silico, in vitro, and in vivo analyses to assess anti-cancerous potential of a bioactive compound from cyanobacterium nostoc sp. MGL001. Front. Pharmacol., 2017, 8, 873.
[http://dx.doi.org/10.3389/fphar.2017.00873] [PMID: 29230175]
[44]
Jeong, J.; Kim, H.; Choi, J. In silico molecular docking and in vivo validation with caenorhabditis elegans to discover molecular initiating events in adverse outcome pathway framework: case study on endocrine-disrupting chemicals with estrogen and androgen receptors. Int. J. Mol. Sci., 2019, 20(5), 1209.
[http://dx.doi.org/10.3390/ijms20051209] [PMID: 30857347]
[45]
Tahghighi, A.; Mohamadi-Zarch, S.M.; Rahimi, H.; Marashiyan, M.; Maleki-Ravasan, N.; Eslamifar, A. In silico and in vivo anti- malarial investigation on 1-(heteroaryl)-2-((5-nitroheteroaryl)methylene) hydrazine derivatives. Malar. J., 2020, 19(1), 231.
[http://dx.doi.org/10.1186/s12936-020-03269-7] [PMID: 32600425]

Rights & Permissions Print Cite
Article Metrics
21
1
© 2024 Bentham Science Publishers | Privacy Policy