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Research Article

游离和 PLGA-PAA 封装的羟基酪醇对 MCF-7 乳腺癌细胞系的抗癌作用研究

卷 22, 期 7, 2022

发表于: 22 December, 2021

页: [657 - 662] 页: 6

弟呕挨: 10.2174/1566524020666201231103826

价格: $65

摘要

目的：乳腺癌是女性最常见的癌症，也是最重要的死亡原因。手术和化疗是乳腺癌的常见治疗方法，但耐药性的增加为其治疗带来了许多挑战。本研究旨在探讨游离和纳米包封的羟基酪醇对MCF-7乳腺癌细胞系的抗癌作用。 方法：合成聚丙交酯-共-乙交酯-共-聚丙烯酸（PLGA-co-PAA）纳米包封羟基酪醇，采用MTT法评价游离和纳米包封的抗增殖和抗肿瘤作用。羟基酪醇。从处理过的和对照的癌细胞中提取 RNA 后，进行 cDNA 合成，并通过实时 PCR 评估 P21、P27 和 Cyclin D1 基因的表达。 结果：研究结果表明，游离（12 ppm 和 72 小时）和纳米封装（10 ppm 和 24 小时）羟基酪醇导致癌细胞死亡 50%（IC50），并随着浓度和时间的增加而增加。此外，游离和纳米封装的羟基酪醇增加了乳腺癌细胞中 P21 和 P27 基因的表达，并降低了 Cyclin D1 的表达。一般来说，纳米封装的羟基酪醇比游离羟基酪醇显示出更多的抗癌功能。 结论：本研究表明羟基酪醇可通过调节细胞周期导致MCF-7乳腺癌细胞死亡。此外，羟基酪醇的纳米包封通过PLGA-co-PAA增强了羟基酪醇的抗癌功能。然而，为了获得更准确的结果，需要对动物模型进行进一步研究。

关键词: 乳腺癌、PLGA-PAA 共聚物、细胞周期、羟基酪醇、纳米封装。

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