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Current Chemical Biology

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ISSN (Print): 2212-7968
ISSN (Online): 1872-3136

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Research Article

Differential Metabolites Markers from Trunking and Stressed Non-Trunking Sago Palm (Metroxylon sagu Rottb.)

Author(s): Hasnain Hussain*, Wei-Jie Yan, Zainab Ngaini, Norzainizul Julaihi, Rina Tommy and Showkat Ahmad Bhawani

Volume 14, Issue 4, 2020

Page: [262 - 278] Pages: 17

DOI: 10.2174/2212796814999200930120925

Price: $65

Abstract

Background: Sago palm is an important agricultural starch-producing crop in Malaysia. The trunk of sago palm is responsible for the the starch to reach maturity for harvesting after ten years. However, there are sago palms that fail to develop thier trunk after 17 years of being planted. This is known as a stressed “non-trunking” sago palm, which reduces the economic value of the palms.

Objective: The study was initiated to compare the differences in metabolite expression between trunking and non-trunking sago palms and secondly to determine the potential metabolite- makers that are related to differential phenotypes of sago palms.

Methods: Metabolites were extracted using various solvents and analysed using NMR spectroscopy and GC-MS spectrometry. Data obtained were subjected to principal component analysis.

Results: The study determined differential metabolites expression in the leaf extracts of normal trunking sago palm compared to the non-trunking palms. Metabolite groups differently expressed between trunking and non-trunking sago palm are oils and waxes, haloalkanes, sulfite esters, phosphonates, phosphoric acid, thiophene ester, terpenes and tocopherols. GC-MS analysis of Jones & Kinghorn extraction method determined two sets of metabolite markers, explaining the differences in metabolites expression of trunking and nontrunking sago palms in ethyl acetate and methanol extract of 89.55% comprising sulfurous ester compounds and 87.04% comprising sulfurous ester, sulfurous acid and cyclohexylmethyl hexyl ester, respectively.

Conclusion: Two sets of metabolite markers were expressed in the trunking and nontrunking sago palms. These metabolites can potentially be used as markers for identifying normal and stressed plants.

Keywords: Non-trunking, sago, metabolites, differential expression, Metroxylon sagu, metabolite markers.

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[1]
Jong F-S. Research for the development of sago palm (Metroxylon sagu Rottb) cultivation in Sarawak, Malaysia Department of Agronomy, Wageningen University & Research, Netherlands,. 1995; p. 135.
[2]
McClatchey W, Manner HI, Elevitch CR, et al. M warburgii (sago palm) Traditional Trees of Pacific Islands: Their Culture Environment, and Use Permanent Agricultural Resources, 2006; 491-512.
[3]
Flach M. Sago palm: Metroxylon sagu Rottb Promoting the conservation and use of underutilized and neglected crops 13 Bioversity International: Institute of Plant Genetics and Crop Plant Research. Gatersleben, Germany/International Plant Genetic Resources Institute, Rome, Italy 1997.
[4]
Fong SS, Khan AJ, Mohamed M, Dos Mohammad AM. The relationship between peat soil characteristics and the growth of sago palm (Metroxylon sagu). Sago Palm 2005; 13: 9-16.
[5]
Okazaki M, Sasaki Y. Soil environment in sago palm forest. In: Ehara H, Toyoda Y, Johnson DV. Sago Palm: Multiple contributions to food security and sustainable livelihoods. Springer Singapore: Singapore 2018; pp. 193-206.
[6]
Apun K, Lihan S, Wong MK, Bilung LM. Microbiological characteristics of trunking and non-trunking sago palm peat soil. In: 1st ASEAN Sago Symposium 2009: Current Trend and Development in Sago Research. Universiti Malaysia Sarawak: Kuching, In: 2009; pp. 29-30.
[7]
Toyota K. Microbial Interactions and Activities Affecting Sago Palm Growth. In: Ehara H, Toyoda Y, Johnson DV, Eds. Sago Palm: Multiple Contributions to Food Security and Sustainable Livelihoods Springer Singapore. Singapore 2018; pp. 207-17.
[8]
Lim LWK, Chung HH, Hussain H, Bujang K. Sago Palm (Metroxylon sagu Rottb.): Now and Beyond. Pertanika J Trop Agric 2019; 42: 435-51.
[9]
van der Greef J, Smilde AK. Symbiosis of chemometrics and metabolomics: Past, present, and future. J Chemometr 2005; 19: 376-86.
[10]
Rosato A, Tenori L, Cascante M, De Atauri Carulla PR, Martins dos Santos VAP, Saccenti E. From correlation to causation: Analysis of metabolomics data using systems biology approaches. Metabolomics 2018; 14: 37.
[11]
Melandri G. AbdElgawad H, Riewe D, Hageman JA, Asard H, Beemster GTS, Kadam N, Jagadish K, Altmann T, Ruyter-Spira C, Bouwmeester H. Biomarkers for grain yield stability in rice under drought stress. J Exp Bot 2019; 71: 669-83.
[12]
Liu J, Liu Y, Wang Y, et al. The combined effects of ethylene and MeJA on metabolic profiling of phenolic compounds in catharanthus roseus revealed by metabolomics analysis. Front Physiol 2016; 7.
[13]
Schwieterman ML, Colquhoun TA, Jaworski EA, et al. Strawberry flavor: diverse chemical compositions, a seasonal influence, and effects on sensory perception. PLoS One 2014; 9: e88446.
[14]
Lim LWK, Chung HH, Hussain H. Complete chloroplast genome sequencing of sago palm (Metroxylon sagu Rottb.): Molecular structures, comparative analysis and evolutionary significance. Gene Rep 2020; 19: 100662.
[15]
Edward AS, Hussain H. Differential expression gene profiling of trunking and non-trunking sago palm In: 2nd Biotechnology Colloquium 2009 Department of Molecular Biology, Faculty of Resource Science and Technology, UNIMAS. 2009; p. 96.
[16]
Hussain H, Kamal MM, Al-Obaidi JR, Hamdin NE, Ngaini Z, Mohd-Yusuf Y. Proteomics of Sago Palm Towards Identifying Contributory Proteins in Stress-Tolerant Cultivar. Protein J 2020; 39: 62-72.
[17]
Nisar M, Hussain H. The protein extraction method of Metroxylon sagu leaf for high-resolution two-dimensional gel electrophoresis and comparative proteomics. Chem Biol Technol Agric 2020; 7: 14.
[18]
Jones WP, Kinghorn AD. Extraction of plant secondary metabolites In: Sarker SD, Latif Z, Gray AI, Eds, Natural Products Isolation Springer. 2006; pp. 323-51.
[19]
Sobolev AP, Brosio E, Gianferri R, Segre AL. Metabolic profile of lettuce leaves by high-field NMR spectra. Magn Reson Chem 2005; 43: 625-38.
[20]
Macomber RS. A Complete Introduction to Modern NMR Spectroscopy Wiley: New York. 1998.
[21]
Shepherd T, Wynne Griffiths D. The effects of stress on plant cuticular waxes. New Phytol 2006; 171: 469-99.
[22]
Denic V, Weissman JS. A molecular caliper mechanism for determining very long-chain fatty acid length. Cell 2007; 130: 663-77.
[23]
Nobusawa T, Okushima Y, Nagata N, Kojima M, Sakakibara H, Umeda M. Synthesis of very-long-chain fatty acids in the epidermis controls plant organ growth by restricting cell proliferation. PLoS Biol 2013; 11: e1001531.
[24]
Taiz L, Zeiger E. Plant Physiology Sinauer Associates. Sunderland, Massachusetts, USA 2002.
[25]
Inamori Y, Muro C, Osaka K, et al. Inhibitory activities of 3-thiophenecarboxylic acid and related compounds on plant growth. Bioscie Biotechnol Biochem 1994; 58: 1336-7.
[26]
McGorrin RJ. Character-impact flavor and off-flavor compounds in foods. In: Marsili R, ed.Flavor, Fragrance, and Odor Analysis CRC Press,. Boca Raton, FL, USA 2012; pp. 207-62.
[27]
Margl L, Tei A, Gyurján I, Wink M. GLC and GLC-MS analysis of thiophene derivatives in plants and in in vitro cultures of Tagetes patula L.(Asteraceae). Zeitschrift für Naturforschung C 2002; 57: 63-71.
[28]
Yu X, Doroghazi JR, Janga SC, et al. Diversity and abundance of phosphonate biosynthetic genes in nature. Proc Natl Acad Sci 2013; 110: 20759-64.
[29]
Jackson T, Burgess T, Colquhoun I, Hardy GS. Action of the fungicide phosphite on Eucalyptus marginata inoculated with Phytophthora cinnamomi. Plant Pathol 2000; 49: 147-54.
[30]
Hincha DK. Effects of α‐tocopherol (vitamin E) on the stability and lipid dynamics of model membranes mimicking the lipid composition of plant chloroplast membranes. FEBS Lett 2008; 582: 3687-92.
[31]
Munné-Bosch S. The role of α-tocopherol in plant stress tolerance. J Plant Physiol 2005; 162: 743-8.
[32]
Čamagajevac IŠ, Pfeiffer TŽ, Maronić DŠ. Abiotic Stress Response in Plants: The Relevance of Tocopherols. In: Gupta D, Palma J, Corpas F(eds). Antioxidants and Antioxidant Enzymes in Higher Plants. Cham: Springer 2018; pp. 233-51.
[33]
Amarowicz R. Squalene: a natural antioxidant? European J Lipid Sci Technol 2009; 111: 411-2.

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