Origanum vulgare L.: In vitro Assessment of Cytotoxicity, Molecular Docking Studies, Antioxidant and Anti-inflammatory Activity in LPS Stimulated RAW 264.7 Cells

Title:Origanum vulgare L.: In vitro Assessment of Cytotoxicity, Molecular Docking Studies, Antioxidant and Anti-inflammatory Activity in LPS Stimulated RAW 264.7 Cells

Volume: 17 Issue: 9

Author(s): Reyaz H. Mir, Gifty Sawhney, Rohini Verma, Bilal Ahmad, Parveen Kumar, Soni Ranjana, Asha Bhagat, Sreedhar Madishetti, Zabeer Ahmed, Sanjay M. Jachak, Sangdun Choi and Mubashir H. Masoodi*

Affiliation:

• Pharmaceutical Chemistry Division, Department of Pharmaceutical Sciences, University of Kashmir, Hazratbal, Srinagar 190006, Kashmir,India

Keywords: Phytoconstituents, IL-6, LPS, traditional medicine, inflammation, anti-inflammatory.

Abstract:

Background: Inflammation involves a dynamic network that is highly regulated by signals that initiate the inflammation process as well as signals that downregulate it. However, an imbalance between the two leads to tissue damage. Throughout the world, inflammatory disease becomes common in the aging society. The drugs which are used clinically have serious side effects. Natural products or compounds derived from natural products show diversity in structure and play an important role in drug discovery and development.

Objective: Oreganum Vulgare is used in traditional medicine for various ailments including respiratory and rheumatic disorders, severe cold, suppression of tumors. The current study aims to evaluate the anti-inflammatory potential by evaluating various in vitro parameters.

Methods: Inflammation-induced in macrophages via LPS is the most accepted model for evaluating the antiinflammatory activity of various plant extracts and lead compounds.

Results: The extracts (OVEE, OVEAF) as well as the isolated compound(OVRA)of Oreganum Vulgare inhibit the pro-inflammatory cytokines (IL-6 and TNF-α) and NO without affecting cell viability.

Conclusion: Our study established that the leaf extracts of Oreganum vulgare L. exhibit anti-inflammatory activity and thus confirm its importance in traditional medicine.

Cite this article as:

Mir H. Reyaz , Sawhney Gifty , Verma Rohini , Ahmad Bilal , Kumar Parveen , Ranjana Soni , Bhagat Asha , Madishetti Sreedhar, Ahmed Zabeer , Jachak M. Sanjay , Choi Sangdun and Masoodi H. Mubashir *, Origanum vulgare L.: In vitro Assessment of Cytotoxicity, Molecular Docking Studies, Antioxidant and Anti-inflammatory Activity in LPS Stimulated RAW 264.7 Cells, Medicinal Chemistry 2021; 17 (9) . https://dx.doi.org/10.2174/1573406416666200904110828