An Overview of Algorithms and Associated Applications for Single Cell RNA-Seq Data Imputation

Title:An Overview of Algorithms and Associated Applications for Single Cell RNA-Seq Data Imputation

Volume: 22 Issue: 5

Author(s): Zarrin Basharat*, Sania Majeed, Humaira Saleem, Ishtiaq Ahmad Khan and Azra Yasmin

Affiliation:

• Jamil–ur–Rahman Center for Genome Research, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi–75270,Pakistan

Keywords: Single cell, RNA-Seq, imputation, algorithms, heterogeneity, analysis.

Abstract: Single cell RNA-Seq technology enables the assessment of RNA expression in individual cells. This makes it popular in experimental biology for gleaning specifications of novel cell types as well as inferring heterogeneity. Experimental data conventionally contains zero counts or dropout events for many single cell transcripts. Such missing data hampers the accurate analysis using standard workflows, designed for massive RNA-Seq datasets. Imputation for single cell datasets is done to infer the missing values. This was traditionally done with ad-hoc code but later customized pipelines, workflows and specialized software appeared for this purpose. This made it easy to benchmark and cluster things in an organized manner. In this review, we have assembled a catalog of available RNASeq single cell imputation algorithms/workflows and associated softwares for the scientific community performing single-cell RNA-Seq data analysis. Continued development of imputation methods, especially using deep learning approaches, would be necessary for eradicating associated pitfalls and addressing challenges associated with future large scale and heterogeneous datasets.

Cite this article as:

Basharat Zarrin*, Majeed Sania , Saleem Humaira , Khan Ahmad Ishtiaq and Yasmin Azra , An Overview of Algorithms and Associated Applications for Single Cell RNA-Seq Data Imputation, Current Genomics 2021; 22 (5) . https://dx.doi.org/10.2174/1389202921999200716104916