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Current Drug Discovery Technologies

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ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

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Research Article

The Effect of Myrtus communis Aqueous Extract-Containing Gel on Wound Healing in Streptozotocin-Induced Diabetic Rats

Author(s): Seyed-Ali Khodaie, Fatemeh Emadi, Mohsen Naseri*, Mohammad Kamalinejad, Seyed Mohammad Riahi, Fatemeh Alijaniha and Mehrdad Roghani*

Volume 18, Issue 4, 2021

Published on: 12 July, 2020

Page: [542 - 547] Pages: 6

DOI: 10.2174/1570163817666200712163956

Price: $65

Abstract

Background: The medicinal plant Myrtus communis L. (Myrtle) has properties, including anti-inflammatory and wound healing in Persian Medicine.

Objective: The objective of this study was to explore the wound healing potential of the local application of a gel containing aqueous extract of the plant berry in streptozotocin (STZ)-induced diabetic rats.

Methods: Seven days after diabetes establishment, full-thickness excision skin wounds were made in normal and diabetic rats where treated groups received topical application of a gel containing 6% aqueous extract of myrtle berries for 3 weeks. The rate of wound healing and the level of epidermal and dermal maturation in the wound tissue were determined.

Results: The results showed that after 3 and 7 days of wound injury, the gel significantly improved wound healing by accelerating epidermal and dermal maturation in diabetic rats with no significant effect in the control group. However, the wounds of all groups almost completely healed after 3 weeks.

Conclusion: These results demonstrate that aqueous extract of myrtle possesses a definite wound healing potential in diabetic conditions. The present findings may suggest the use of topical myrtle berries aqueous extract gel 6% to treat and manage intractable diabetic wounds.

Keywords: Myrtus communis, streptozotocin, wound healing, diabetes mellitus, persian medicine, Diabetic foot ulcers (DFUs).

Graphical Abstract

[1]
Zhang Y, McClain SA, Lee H-M, et al. A novel chemically modified curcumin “normalizes” wound-healing in rats with experimentally induced type I diabetes: initial studies. J Diabetes Res 2016.20165782904
[http://dx.doi.org/10.1155/2016/5782904] [PMID: 27190999]
[2]
Boulton AJ. Pressure and the diabetic foot: Clinical science and offloading techniques. Am J Surg 2004; 187(5A): 17S-24S.
[http://dx.doi.org/10.1016/S0002-9610(03)00297-6] [PMID: 15147987]
[3]
Rathur HM, Boulton AJ. The diabetic foot. Clin Dermatol 2007; 25(1): 109-20.
[http://dx.doi.org/10.1016/j.clindermatol.2006.09.015] [PMID: 17276208]
[4]
McDaniel JC, Browning KK. Smoking, chronic wound healing, and implications for evidence-based practice. J Wound Ostomy Continence Nurs 2014; 41(5): 415-23.
[http://dx.doi.org/10.1097/WON.0000000000000057] [PMID: 25188797]
[5]
Cianfarani F, Toietta G, Di Rocco G, Cesareo E, Zambruno G, Odorisio T. Diabetes impairs adipose tissue-derived stem cell function and efficiency in promoting wound healing. Wound Repair Regen 2013; 21(4): 545-53.
[http://dx.doi.org/10.1111/wrr.12051] [PMID: 23627689]
[6]
Yadollah-Damavandi S, Chavoshi-Nejad M, Jangholi E, et al. Topical Hypericum perforatum improves tissue regeneration in full-thickness excisional wounds in diabetic rat model. Evid Based Complement Alternat Med 2015.2015245328
[http://dx.doi.org/10.1155/2015/245328] [PMID: 26417372]
[7]
Hajiheydari MR, Yarmohammadi ME, Izadi P, et al. Effect of Nepeta bracteata Benth. on allergic rhinitis symptoms: A randomized double-blind clinical trial. J Res Med Sci 2017; 22: 128.
[http://dx.doi.org/10.4103/jrms.JRMS_316_17] [PMID: 29259639]
[8]
Soltanian AR, Mehdibarzi D, Faghihzadeh S, Naseri M, Gerami A. Mixture of Arnebia euchroma and Matricaria chamomilla (Marhame-Mafasel) for pain relief of osteoarthritis of the knee–A two-treatment, two-period crossover trial. Archives of medical science. AMS 2010; 6(6): 950.
[http://dx.doi.org/10.5114/aoms.2010.19307] [PMID: 22427772]
[9]
Ahmadi A, Mohagheghi M, Karimi M, et al. Therapeutic effects of HESA-A in patients with end-stage metastatic cancers. Integr Cancer Ther 2010; 9(1): 32-5.
[http://dx.doi.org/10.1177/1534735409357934] [PMID: 20150223]
[10]
Hosein Farzaei M, Abbasabadi Z, Reza Shams-Ardekani M, Abdollahi M, Rahimi R. A comprehensive review of plants and their active constituents with wound healing activity in traditional Iranian medicine. Wounds 2014; 26(7): 197-206.
[PMID: 25856320]
[11]
Romani A, Coinu R, Carta S, et al. Evaluation of antioxidant effect of different extracts of Myrtus communis L. Free Radic Res 2004; 38(1): 97-103.
[http://dx.doi.org/10.1080/10715760310001625609] [PMID: 15061659]
[12]
Ghaffari F, Naseri M, Khodadoost M. Traditional Iranian medicine and the need for its revival and development 2010.
[13]
Ghaffari F, Naseri M, Jafari Hajati R, Zargaran A. Rhazes, a pioneer in contribution to trials in medical practice. Acta Med Hist Adriat 2017; 15(2): 261-70.
[http://dx.doi.org/10.31952/amha.15.2.4] [PMID: 29402116]
[14]
Avicenna H. Kitab al- Qanun fi al- Tibb. Beirut.Alaalamy Publication. 2005; Vol. 4: pp. 230-44. The Cannon of Medicine [In Arabic].
[15]
Aazam Khan Chashti M. Exir-E-Aazam. Tehran: Institue Of Medical History & Islamic And Complemantory Medicine Studies.Iran Univercity. 2008; Vol. 4: pp. 614-41. Grate Panacea [In Persian].
[16]
Hakim Mohammad. Zakhira-Kamelah The Treasury of Perfection Correction and research: Mirsalehian HTehran: Almaeipublications 2013.
[17]
Sedaghat R, Ghosian Moghadam M, Naseri M, Davati A. Histological evaluation of the anti-inflammatory effects of Alkanna tinctoria on the cutaneous wounds healing in rat. Hormozgan Med J 2011; 14(4): 281-9.
[18]
Aliasl J, Barikbin B, Khoshzaban F, et al. Effect of Arnebia euchroma ointment on post-laser wound healing in rats. J Cosmet Laser Ther 2015; 17(1): 41-5.
[http://dx.doi.org/10.3109/14764172.2014.968583] [PMID: 25260137]
[19]
Khodaie S-A, Kamalinejad M, Emadi F, Naseri M. Prioritization of effective Materia Medica on diabetic foot ulcer in Persian medicine. Daneshvar 2018; 25(132): 45-54. [In Persian].
[20]
Alipour G, Dashti S, Hosseinzadeh H. Review of pharmacological effects of Myrtus communis L. and its active constituents. Phytother Res 2014; 28(8): 1125-36.
[http://dx.doi.org/10.1002/ptr.5122] [PMID: 24497171]
[21]
Asgarpanah J, Ariamanesh A. Phytochemistry and pharmacological properties of Myrtus communis L. Indian J Tradit Knowl 2015; 1(1): 82-7.
[22]
Aleksic V, Knezevic P. Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiol Res 2014; 169(4): 240-54.
[http://dx.doi.org/10.1016/j.micres.2013.10.003] [PMID: 24291016]
[23]
Mahboubi M. Myrtus communis L. and its application in treatment of Recurrent Aphthous Stomatitis. J Ethnopharmacol 2016; 193: 481-9.
[http://dx.doi.org/10.1016/j.jep.2016.09.054] [PMID: 27693775]
[24]
Mimica-Dukić N, Bugarin D, Grbović S, et al. Essential oil of Myrtus communis L. as a potential antioxidant and antimutagenic agents. Molecules 2010; 15(4): 2759-70.
[http://dx.doi.org/10.3390/molecules15042759] [PMID: 20428077]
[25]
Sumbul S, Ahmad MA, Asif M, Akhtar M. Myrtus communis Linn.-A review. Ind J Natur Prod Res 2011; 2(4): 395-402.
[26]
Mobli M, Haririan I, Amin G, Kamalinejad M, Hajimahmoodi M. Stability of polyphenols in myrtle berries syrup, a traditional Iranian medicine Tradition integ medic 2016; 1(1): 35-9.
[27]
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol 1999; 299: 152-78.
[http://dx.doi.org/10.1016/S0076-6879(99)99017-1]
[28]
Lin J-Y, Tang C-Y. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem 2007; 101(1): 140-7.
[http://dx.doi.org/10.1016/j.foodchem.2006.01.014]
[29]
Wang J, Wang L, Zhou J, Qin A, Chen Z. The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice. Biomed Pharmacother 2018; 106: 1250-7.
[http://dx.doi.org/10.1016/j.biopha.2018.07.063] [PMID: 30119194]
[30]
Lee Y-H, Chang J-J, Chien C-T, Yang M-C, Chien H-F. Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats. Exp Diabetes Res 2012; 2012: 504693.
[http://dx.doi.org/10.1155/2012/504693] [PMID: 22919368]
[31]
Yates CC, Whaley D, Babu R, et al. The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models. Biomaterials 2007; 28(27): 3977-86.
[http://dx.doi.org/10.1016/j.biomaterials.2007.05.008] [PMID: 17561250]
[32]
Al-Bayaty F, Abdulla MA. A comparison of wound healing rate following treatment with aftamed and chlorine dioxide gels in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med 2012; 2012: 468764.
[http://dx.doi.org/10.1155/2012/468764] [PMID: 22666291]
[33]
Nayak BS, Pereira LP, Maharaj D. Wound healing activity of Carica papaya L experimentally induced diabetic rats 2007; 45(8): 739-43.
[34]
Ponrasu T, Suguna L. Efficacy of Annona squamosa on wound healing in streptozotocin-induced diabetic rats. Int Wound J 2012; 9(6): 613-23.
[http://dx.doi.org/10.1111/j.1742-481X.2011.00924.x] [PMID: 22233431]
[35]
Cao Y, Gang X, Sun C, Wang G. Mesenchymal stem cells improve healing of diabetic foot ulcer. J Diabetes Res 2017.20179328347
[http://dx.doi.org/10.1155/2017/9328347] [PMID: 28386568]
[36]
Neville RF, Kayssi A, Buescher T, Stempel MS. The diabetic foot. Curr Probl Surg 2016; 53(9): 408-37.
[http://dx.doi.org/10.1067/j.cpsurg.2016.07.003] [PMID: 27687301]
[37]
Arya AK, Tripathi R, Kumar S, Tripathi K. Recent advances on the association of apoptosis in chronic non healing diabetic wound. World J Diabetes 2014; 5(6): 756-62.
[http://dx.doi.org/10.4239/wjd.v5.i6.756] [PMID: 25512778]
[38]
El-Refaei MF, Abduljawad SH, Alghamdi AH. Alternative medicine in diabetes-role of angiogenesis, oxidative stress, and chronic inflammation. Rev Diabet Stud 2014; 11(3-4): 231-44.
[http://dx.doi.org/10.1900/RDS.2014.11.231] [PMID: 26177484]
[39]
Hu SC-S, Lan CE. High-glucose environment disturbs the physiologic functions of keratinocytes: Focusing on diabetic wound healing. J Dermatol Sci 2016; 84(2): 121-7.
[http://dx.doi.org/10.1016/j.jdermsci.2016.07.008] [PMID: 27461757]
[40]
Eo H, Lee H-J, Lim Y. Ameliorative effect of dietary genistein on diabetes induced hyper-inflammation and oxidative stress during early stage of wound healing in alloxan induced diabetic mice. Biochem Biophys Res Commun 2016; 478(3): 1021-7.
[http://dx.doi.org/10.1016/j.bbrc.2016.07.039] [PMID: 27431618]
[41]
Jabri M-A, Tounsi H, Rtibi K, Marzouki L, Sakly M, Sebai H. Ameliorative and antioxidant effects of myrtle berry seed (Myrtus communis) extract during reflux-induced esophagitis in rats. Pharm Biol 2016; 54(9): 1575-85.
[http://dx.doi.org/10.3109/13880209.2015.1107748] [PMID: 26810811]
[42]
Rosa A, Melis MP, Deiana M, et al. Protective effect of the oligomeric acylphloroglucinols from Myrtus communis on cholesterol and human low density lipoprotein oxidation. Chem Phys Lipids 2008; 155(1): 16-23.
[http://dx.doi.org/10.1016/j.chemphyslip.2008.04.005] [PMID: 18498764]
[43]
Hashemipour MA, Lotfi S, Torabi M, et al. Evaluation of the effects of three plant species (Myrtus Communis L., Camellia Sinensis L., Zataria Multiflora Boiss.) on the healing process of intraoral ulcers in rats. J Dent (Shiraz) 2017; 18(2): 127-35.
[PMID: 28620637]

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