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Current Chromatography

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ISSN (Print): 2213-2406
ISSN (Online): 2213-2414

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Research Article

Chromatographic Assessment of Polyphenolic Profile and Total Phenolic Content and Antioxidant Activity of Common Leafy Vegetables in Bangladesh

Author(s): Mohammad Khairul Alam*, Rumana Tuli, Mohammad Sharif Khan, Abu Tareq Mohammad Abdullah, Maksuda Khatun, Mohammad Akhtaruzzaman and Sheikh Nazrul Islam

Volume 7, Issue 1, 2020

Page: [40 - 50] Pages: 11

DOI: 10.2174/2213240607999200421144940

Price: $65

Abstract

Background: Polyphenolic compounds are known to provide health benefits and protect against degenerative chronic diseases. Utilization and identification of foods with a high content of these compounds are gaining greater attention nowadays.

Objective: The present study reports the total phenolic content (TPC), polyphenolic composition and antioxidant activity (DPPH, FRAP and TEAC) of 10 commonly consumed leafy vegetables growing in Bangladesh.

Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Folin-Ciocalteu method was used for the analysis of TPC, and quantification of polyphenolic components was done by high-performance liquid chromatography (HPLC- DAD). Additionally, antioxidant activities of the selected vegetables were also analysed by utilizing DPPH, FRAP & TEAC.

Results and Discussion: TPC ranged from 23.64 ± 1.20 to 45.59 ± 3.04 mg gallic acid equivalent (GAE)/g freeze-dried sample (fds). The polyphenolic spectrum ranged from 0.30 ± 0.02 to 647.42 ± 147.12 mg/100 g fds; quantity and spectrum of which varied in the vegetables. Among the studied vegetables, Centella asiatica contained the highest amount of TPC (45.59 ± 3.04 mg GAE/g fds) and also exhibited high antioxidant capacities, as documented by DPPH, FRAP and TEAC assays. Moreover, Principal component analysis (PCA) of investigated variables clearly separated Centella asiatica from other samples.

Conclusion: Phenolic compounds being strong antioxidants reduce the risk of chronic diseases and the finding of this study would aware the people to take vegetables rich in phenolics. It would also fill up the data gap in the existing food composition table of Bangladesh.

Keywords: Antioxidants, HPLC-DAD, leafy vegetables, polyphenolic profile, principal component analysis, total phenolic content.

[1]
Alam, M.K.; Rana, Z.H.; Islam, S.N. Comparison of the proximate composition, total carotenoids and total polyphenol content of nine orange-fleshed sweet potato varieties grown in Bangladesh. Foods, 2016, 5(3), 64.
[http://dx.doi.org/10.3390/foods5030064] [PMID: 28231159]
[2]
Alam, M.K.; Rana, Z.H.; Akhtaruzzaman, M. Chemical composition and fatty acid profile of bangladeshi beef at retail. Int. Food Res. J., 2017, 24(5), 1897-1902.
[3]
Alam, M.K.; Rana, Z.H.; Akhtaruzzaman, M. Comparison of muscle and subcutaneous tissue fatty acid composition of bangladeshi nondescript deshi bulls finished on pasture diet. J. Chem., 2017, 2017, 1-6.
[http://dx.doi.org/10.1155/2017/8579387]
[4]
Alam, M.K.; Rana, Z.H.; Islam, S.N.; Akhtaruzzaman, M. Total phenolic content and antioxidant activity of methanolic extract of selected wild leafy vegetables grown in Bangladesh: A cheapest source of antioxidants. Potravin. Slovak J. Food Sci., 2019, 13(1), 287-293.
[http://dx.doi.org/10.5219/1107]
[5]
Rana, Z.H.; Alam, M.K.; Akhtaruzzaman, M. Nutritional composition, total phenolic content, antioxidant and α-amylase inhibitory activities of different fractions of selected wild edible plants. Antioxidants, 2019, 8(7), 203.
[http://dx.doi.org/10.3390/antiox8070203] [PMID: 31266183]
[6]
Lutz, M.; Hernández, J.; Henríquez, C. Phenolic content and antioxidant capacity in fresh and dry fruits and vegetables grown in Chile. CYTA J. Food, 2015, 13(4), 541-547.
[http://dx.doi.org/10.1080/19476337.2015.1012743]
[7]
Murimi, M.W.; Kanyi, M.G.; Mbogori, T.N.; Amin, M.R.; Rana, Z.H.; Nguyen, B.; Moyeda-Carabaza, A.F. Voices and perspectives of rural hispanic households on food insecurity in west Texas: A qualitative study. J. Hunger Environ. Nutr., 2018, 1-18.
[http://dx.doi.org/10.1080/19320248.2018.1434105]
[8]
Nguyen, B.; Murimi, M.; Rana, Z.H.; Lee, H.; Halloran, R. Impact of a nutrition education intervention on nutrition knowledge and dietary intake of fruits, vegetables, and milk among fourth and fifth-grade elementary school children. J. Nutr. Educ. Behav., 2016, 48(7), S79.
[http://dx.doi.org/10.1016/j.jneb.2016.04.211]
[9]
Andarwulan, N.; Kurniasih, D.; Apriady, R.A.; Rahmat, H.; Roto, A.V.; Bolling, B.W. Polyphenols, carotenoids, and ascorbic acid in underutilized medicinal vegetables. J. Funct. Foods, 2012, 4(1), 339-347.
[http://dx.doi.org/10.1016/j.jff.2012.01.003]
[10]
Działo, M.; Mierziak, J.; Korzun, U.; Preisner, M.; Szopa, J.; Kulma, A. The potential of plant phenolics in prevention and therapy of skin disorders. Int. J. Mol. Sci., 2016, 17(2), 160.
[http://dx.doi.org/10.3390/ijms17020160] [PMID: 26901191]
[11]
Savic Gajic, I.; Savic, I.; Boskov, I.; Žerajić, S.; Markovic, I.; Gajic, D. Optimization of ultrasound-assisted extraction of phenolic compounds from black locust (Robiniae Pseudoacaciae) flowers and comparison with conventional methods. Antioxidants, 2019, 8(8), 248.
[http://dx.doi.org/10.3390/antiox8080248] [PMID: 31357622]
[12]
Savic, I.M.; Nikolic, I.L.; Savic-Gajic, I.M.; Kundakovic, T.D. Modeling and optimization of bioactive compounds from chickpea seeds (Cicer arietinum L). Sep. Sci. Technol., 2018, 54(5), 837-846.
[http://dx.doi.org/10.1080/01496395.2018.1520720]
[13]
Dutta, A.K.; Gope, P.S.; Banik, S.; Makhnoon, S.; Siddiquee, M.A.; Kabir, Y. Antioxidant properties of ten high yielding rice varieties of Bangladesh. Asian Pac. J. Trop. Biomed., 2012, 2(1)(Suppl.), S99-S103.
[http://dx.doi.org/10.1016/S2221-1691(12)60137-3]
[14]
Sharmin, H.; Nazma, S.; Mohiduzzaman, M.; Cadi, P.B. Antioxidant capacity and total phenol content of commonly consumed selected vegetables of Bangladesh. Malays. J. Nutr., 2011, 17(3), 377-383.
[PMID: 22655459]
[15]
Mamun, S.; Shaheen, N.; Basak, T.A.; Mohiduzzaman, M.; Banu, C.P.; Takano-Ishikawa, Y. Hydrophilic antioxidant capacities and total phenol content of seasonal fruits of Bangladesh. Malays. J. Nutr., 2012, 18(3), 355-362.
[PMID: 24568076]
[16]
Tukun, A.B.; Shaheen, N.; Banu, C.P.; Mohiduzzaman, M.; Islam, S.; Begum, M. Antioxidant capacity and total phenolic contents in hydrophilic extracts of selected Bangladeshi medicinal plants.Asian Pac. J. Trop. Med. 2014, 7S1(S1), S568-S573.
[http://dx.doi.org/10.1016/S1995-7645(14)60291-1] [PMID: 25312185]
[17]
Islam, S.N.; Khan, M.N.I.; Akhtaruzzaman, M. A food composition database for bangladesh with special reference to selected ethnic foods; Institute of nutrition and food science: Dhaka , 2010.
[18]
Islam, S.N.; Khan, M.N.I.; Akhtaruzzaman, M. Food composition tables and database for bangladesh with special reference to selected ethnic foods; Institute of nutrition and food science: Dhaka, , 2012.
[19]
Hossain, A.; Begum, P.; Salma Zannat, M.; Hafizur Rahman, M.; Ahsan, M.; Islam, S.N. Nutrient composition of strawberry genotypes cultivated in a horticulture farm. Food Chem., 2016, 199, 648-652.
[http://dx.doi.org/10.1016/j.foodchem.2015.12.056] [PMID: 26776020]
[20]
Islam, S.N.; Nusrat, T.; Begum, P.; Ahsan, M. Carotenoids and β-carotene in orange fleshed sweet potato: A possible solution to vitamin A deficiency. Food Chem., 2016, 199, 628-631.
[http://dx.doi.org/10.1016/j.foodchem.2015.12.057] [PMID: 26776017]
[21]
Tariqul Islam Shajib, M.; Kawser, M.; Nuruddin Miah, M.; Begum, P.; Bhattacharjee, L.; Hossain, A.; Fomsgaard, I.S.; Islam, S.N. Nutritional composition of minor indigenous fruits: cheapest nutritional source for the rural people of Bangladesh. Food Chem., 2013, 140(3), 466-470.
[http://dx.doi.org/10.1016/j.foodchem.2012.11.035] [PMID: 23601393]
[22]
Uddin, M.N.; Ahmed, N.U.; Rahman, M.A.; Akter, R.; Akter, R. Antioxidative potential of the polyphenolics of stephania japonica var. discolor (Blume) forman: A chromatographic (high-performance liquid chromatography) and spectrophotometric measure. Int. J. Food Prop., 2016, 19(4), 911-928.
[http://dx.doi.org/10.1080/10942912.2015.1048355]
[23]
Fu, L.; Xu, B.T.; Xu, X.R.; Gan, R.Y.; Zhang, Y.; Xia, E.Q.; Li, H.B. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chem., 2011, 129(2), 345-350.
[http://dx.doi.org/10.1016/j.foodchem.2011.04.079] [PMID: 30634236]
[24]
Ismail, A.; Marjan, Z.M.; Foong, C.W. Total antioxidant activity and phenolic content in selected vegetables. Food Chem., 2004, 87(4), 581-586.
[http://dx.doi.org/10.1016/j.foodchem.2004.01.010]
[25]
Khanam, U.K.S.; Oba, S.; Yanase, E.; Murakami, Y. Phenolic acids, flavonoids and total antioxidant capacity of selected leafy vegetables. J. Funct. Foods, 2012, 4(4), 979-987.
[http://dx.doi.org/10.1016/j.jff.2012.07.006]
[26]
Kongkachuichai, R.; Charoensiri, R.; Yakoh, K.; Kringkasemsee, A.; Insung, P. Nutrients value and antioxidant content of indigenous vegetables from Southern Thailand. Food Chem., 2015, 173, 838-846.
[http://dx.doi.org/10.1016/j.foodchem.2014.10.123] [PMID: 25466097]
[27]
Jiménez-Aguilar, D.M.; Grusak, M.A. Minerals, Vitamin C, Phenolics, flavonoids and antioxidant activity of amaranthus leafy vegetables. J. Food Compos. Anal., 2017, 58, 33-39.
[http://dx.doi.org/10.1016/j.jfca.2017.01.005]
[28]
Shaheen, N.; Rahim, A.T.M.; Mohiduzzaman, M.; Banu, C.P.; Bari, M.L.; Tukun, A.B.; Mannan, M.A.; Bhattacharjee, L.; Stadlmayr, B. Food composition table for Bangladesh, 2nd ed; Institute of Nutrition and Food Science: Dhaka, 2014.
[29]
Longvah, T.; Ananthan, R.; Bhaskarachary, K.; Venkalah, K. Indian Food Composition Tables; National Institute of Nutrition: Hyderbad, 2017.

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