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Infectious Disorders - Drug Targets

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ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

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Case Report

Successful Control of a Co-Infection Caused by Candida albicans and Pseudomonas aeruginosa in Keratitis

Author(s): Debarati Paul, Suman Saha, Neelam Singh, Jayansgu Sengupta and Santi M. Mandal*

Volume 21, Issue 2, 2021

Published on: 18 March, 2020

Page: [284 - 288] Pages: 5

DOI: 10.2174/1871526520666200318111957

Price: $65

Abstract

Introduction: Nowadays, the co-infection of different classes of pathogens is a major concern. The objective of this study was to develop a successful therapy for keratitis caused by the co-infection of Candida sp. with Pseudomonas sp, which is difficult to cure. The study is based on a 47 years old male farmer showing redness and watering in the right eye for 15-days.

Methods: The microbiological examination was performed to isolate the causative organisms, i.e. Pseudomonas aeruginosa and Candida albicans. They were cultured separately along with their co-culture and treated with ciprofloxacin and amphotericin B during the growing stage to predict a definite cure.

Results: Scanning electron microscope (SEM) results confirmed the inter-specific interaction between the two different types of microorganisms. Amphotericin-B and Ciprofloxacin showed the least MIC value for both organisms in co-culture.

Conclusion: Treatment with Amphotericin-B and 5% ciprofloxacin effectively hindered the growth of Pseudomonas aeruginosa and Candida albicans, the co-infection of which caused keratitis. This therapy may be successfully implied for such cases of co-infection in the future.

Keywords: Amphotericin-B, Candida albicans, ciprofloxacin, co-infection, keratitis, Pseudomonas aeruginosa.

Graphical Abstract

[1]
Whitcher, J.P.; Srinivasan, M.; Upadhyay, M.P. Corneal blindness: a global perspective. Bull. World Health Organ., 2001, 79(3), 214-221.
[PMID: 11285665]
[2]
Birger, R.B.; Kouyos, R.D.; Cohen, T.; Griffiths, E.C.; Huijben, S.; Mina, M.J.; Volkova, V.; Grenfell, B.; Metcalf, C.J.E. Erratum to: ‘The potential impact of coinfection on antimicrobial chemotherapy and drug resistance’. Trends Microbiol., 2015, 23(11), 742.
[http://dx.doi.org/10.1016/j.tim.2015.09.001] [PMID: 29482745]
[3]
Dart, J.K. Predisposing factors in microbial keratitis: the significance of contact lens wear. Br. J. Ophthalmol., 1988, 72(12), 926-930.
[http://dx.doi.org/10.1136/bjo.72.12.926] [PMID: 3147696]
[4]
Wilhelmus, K.R. Epidemiology of ocular infections.Duane’s Foundations of Clinical Ophthalmology; Baum, J.; Liesegang, T.J., Eds.; Lippincott Williams & Wilkins: Philadelphia, 1998, Vol. 2, pp. 1-46.
[5]
Wong, T.Y.; Ng, T.P.; Fong, K.S.; Tan, D.T. Risk factors and clinical outcomes between fungal and bacterial keratitis: a comparative study. CLAO J., 1997, 23(4), 275-281.
[PMID: 9348453]
[6]
Bharathi, M.J.; Ramakrishnan, R.; Meenakshi, R.; Padmavathy, S.; Shivakumar, C.; Srinivasan, M. Microbial keratitis in South India: influence of risk factors, climate, and geographical variation. Ophthalmic Epidemiol., 2007, 14(2), 61-69.
[http://dx.doi.org/10.1080/09286580601001347] [PMID: 17464852]
[7]
Frey-Klett, P.; Burlinson, P.; Deveau, A.; Barret, M.; Tarkka, M.; Sarniguet, A. Bacterial-fungal interactions: hyphens between agricultural, clinical, environmental, and food microbiologists. Microbiol. Mol. Biol. Rev., 2011, 75(4), 583-609.
[http://dx.doi.org/10.1128/MMBR.00020-11] [PMID: 22126995]
[8]
Gopinathan, U.; Sharma, S.; Garg, P.; Rao, G.N. Review of epidemiological features, microbiological diagnosis and treatment outcome of microbial keratitis: experience of over a decade. Indian J. Ophthalmol., 2009, 57(4), 273-279.
[http://dx.doi.org/10.4103/0301-4738.53051] [PMID: 19574694]
[9]
Kim, J.H.; Shin, H.H.; Song, J.S.; Kim, H.M. Infectious keratitis caused by Stenotrophomonas maltophilia and yeast simultaneously. Cornea, 2006, 25(10), 1234-1236.
[http://dx.doi.org/10.1097/01.ico.0000226361.11431.5a] [PMID: 17172906]
[10]
Joseph, J.; Chaurasia, S.; Sharma, S. Case Report: Corneal coinfection with fungus and Amoeba: Report of two patients and literature review. Am. J. Trop. Med. Hyg., 2018, 99(3), 805-808.
[http://dx.doi.org/10.4269/ajtmh.18-0158] [PMID: 30014813]
[11]
Sharma, R.; Jhanji, V.; Satpathy, G.; Sharma, N.; Khokhar, S.; Agarwal, T. Coinfection with Acanthamoeba and Pseudomonas in contact lens-associated keratitis. Optom. Vis. Sci., 2013, 90(2), e53-e55.
[http://dx.doi.org/10.1097/OPX.0b013e31827f15b4] [PMID: 23334312]
[12]
Qiu, W.Y.; Yao, Y.F. Mycotic keratitis caused by concurrent infections of Exserohilum mcginnisii and Candida parapsilosis. BMC Ophthalmol., 2013, 13(1), 37.
[http://dx.doi.org/10.1186/1471-2415-13-37] [PMID: 23915416]
[13]
Yeh, D.L.; Stinnett, S.S.; Afshari, N.A. Analysis of bacterial cultures in infectious keratitis, 1997 to 2004. Am. J. Ophthalmol., 2006, 142(6), 1066-1068.
[http://dx.doi.org/10.1016/j.ajo.2006.06.056] [PMID: 17157596]
[14]
Samanta, T.; Roymahapatra, G.; Porto, W.F.; Seth, S.; Ghorai, S.; Saha, S.; Sengupta, J.; Franco, O.L.; Dinda, J.; Mandal, S.M. N, N′-Olefin functionalized bis-imidazolium gold(I) salt is an efficient candidate to control keratitis-associated eye infection. PLoS One, 2013, 8(3), e58346.
[http://dx.doi.org/10.1371/journal.pone.0058346] [PMID: 23554886]
[15]
Schommer, N.N.; Gallo, R.L. Structure and function of the human skin microbiome. Trends Microbiol., 2013, 21(12), 660-668.
[http://dx.doi.org/10.1016/j.tim.2013.10.001] [PMID: 24238601]
[16]
Biswas, K.; Hoggard, M.; Jain, R.; Taylor, M.W.; Douglas, R.G. The nasal microbiota in health and disease: variation within and between subjects. Front. Microbiol., 2015, 9, 134.
[http://dx.doi.org/10.3389/fmicb.2015.00134] [PMID: 25784909]
[17]
Sengupta, J.; Saha, S.; Khetan, A.; Sarkar, S.K.; Mandal, S.M. Effects of lactoferricin B against keratitis-associated fungal biofilms. J. Infect. Chemother., 2012, 18(5), 698-703.
[http://dx.doi.org/10.1007/s10156-012-0398-3] [PMID: 22410856]
[18]
Shirtliff, M.E.; Peters, B.M.; Jabra-Rizk, M.A. Cross-kingdom interactions: Candida albicans and bacteria. FEMS Microbiol. Lett., 2009, 299(1), 1-8.
[http://dx.doi.org/10.1111/j.1574-6968.2009.01668.x] [PMID: 19552706]
[19]
Azoulay, E.; Timsit, J.F.; Tafflet, M.; de Lassence, A.; Darmon, M.; Zahar, J.R.; Adrie, C.; Garrouste-Orgeas, M.; Cohen, Y.; Mourvillier, B.; Schlemmer, B. Candida colonization of the respiratory tract and subsequent pseudomonas ventilator-associated pneumonia. Chest, 2006, 129(1), 110-117.
[http://dx.doi.org/10.1378/chest.129.1.110] [PMID: 16424420]
[20]
Nseir, S.; Jozefowicz, E.; Cavestri, B.; Sendid, B.; Di Pompeo, C.; Dewavrin, F.; Favory, R.; Roussel-Delvallez, M.; Durocher, A. Impact of antifungal treatment on Candida-Pseudomonas interaction: a preliminary retrospective case-control study. Intensive Care Med., 2007, 33(1), 137-142.
[http://dx.doi.org/10.1007/s00134-006-0422-0] [PMID: 17115135]
[21]
Baena-Monroy, T.; Moreno-Maldonado, V.; Franco-Martínez, F.; Aldape-Barrios, B.; Quindós, G.; Sánchez-Vargas, L.O. Candida albicans, Staphylococcus aureus and Streptococcus mutans colonization in patients wearing dental prosthesis. Med. Oral Patol. Oral Cir. Bucal, 2005, 10(Suppl. 1), E27-E39.
[PMID: 15800465]
[22]
Bamford, C.V.; d’Mello, A.; Nobbs, A.H.; Dutton, L.C.; Vickerman, M.M.; Jenkinson, H.F. Streptococcus gordonii modulates Candida albicans biofilm formation through intergeneric communication. Infect. Immun., 2009, 77(9), 3696-3704.
[http://dx.doi.org/10.1128/IAI.00438-09] [PMID: 19528215]
[23]
Boris, S.; Barbés, C. Role played by lactobacilli in controlling the population of vaginal pathogens. Microbes Infect., 2000, 2(5), 543-546.
[http://dx.doi.org/10.1016/S1286-4579(00)00313-0] [PMID: 10865199]
[24]
Högenauer, C.; Hammer, H.F.; Krejs, G.J.; Reisinger, E.C. Mechanisms and management of antibiotic-associated diarrhea. Clin. Infect. Dis., 1998, 27(4), 702-710.
[http://dx.doi.org/10.1086/514958] [PMID: 9798020]
[25]
Cugini, C.; Calfee, M.W.; Farrow, J.M., III; Morales, D.K.; Pesci, E.C.; Hogan, D.A. Farnesol, a common sesquiterpene, inhibits PQS production in Pseudomonas aeruginosa. Mol. Microbiol., 2007, 65(4), 896-906.
[http://dx.doi.org/10.1111/j.1365-2958.2007.05840.x] [PMID: 17640272]
[26]
Machida, K.; Tanaka, T. Farnesol-induced generation of reactive oxygen species dependent on mitochondrial transmembrane potential hyperpolarization mediated by F(0)F(1)-ATPase in yeast. FEBS Lett., 1999, 462(1-2), 108-112.
[http://dx.doi.org/10.1016/S0014-5793(99)01506-9] [PMID: 10580101]
[27]
Hogan, D.A.; Vik, A.; Kolter, R. A Pseudomonas aeruginosa quorum-sensing molecule influences Candida albicans morphology. Mol. Microbiol., 2004, 54(5), 1212-1223.
[http://dx.doi.org/10.1111/j.1365-2958.2004.04349.x] [PMID: 15554963]
[28]
Tu, E.Y.; Joslin, C.E.; Nijm, L.M.; Feder, R.S.; Jain, S.; Shoff, M.E. Polymicrobial keratitis: Acanthamoeba and infectious crystalline keratopathy. Am. J. Ophthalmol., 2009, 148(1), 13-9.e2.
[http://dx.doi.org/10.1016/j.ajo.2009.01.020] [PMID: 19327742]
[29]
Pate, J.C.; Jones, D.B.; Wilhelmus, K.R. Prevalence and spectrum of bacterial co-infection during fungal keratitis. Br. J. Ophthalmol., 2006, 90(3), 289-292.
[http://dx.doi.org/10.1136/bjo.2005.081869] [PMID: 16488946]
[30]
Mejia-Lopez, H.; Perez-Cano, H.J.; Pantoja-Melendez, C. Medina- Hernández, E.O.; Gutiérrez- Sereno, J.J.; Bautista-de Lucio, V.M.; Coinfection Bacteria-Fungus Causing Corneal Ulcer and Keratitis. Invest. Ophthalmol. Vis. Sci., 2010, 51, 2432.
[31]
Gupta, N.; Samantaray, J.C.; Duggal, S.; Srivastava, V.; Dhull, C.S.; Chaudhary, U. Acanthamoeba keratitis with Curvularia co-infection. Indian J. Med. Microbiol., 2010, 28(1), 67-71.
[http://dx.doi.org/10.4103/0255-0857.58736] [PMID: 20061771]
[32]
Schaumberg, D.A.; Snow, K.K.; Dana, M.R. The epidemic of Acanthamoeba keratitis: where do we stand? Cornea, 1998, 17(1), 3-10.
[http://dx.doi.org/10.1097/00003226-199801000-00001] [PMID: 9436873]
[33]
Sharma, S.; Garg, P.; Rao, G.N. Patient characteristics, diagnosis, and treatment of non-contact lens related Acanthamoeba keratitis. Br. J. Ophthalmol., 2000, 84(10), 1103-1108.
[http://dx.doi.org/10.1136/bjo.84.10.1103] [PMID: 11004092]
[34]
Lim, N.C.; Lim, D.K.; Ray, M. Polymicrobial versus monomicrobial keratitis: a retrospective comparative study. Eye Contact Lens, 2013, 39(5), 348-354.
[http://dx.doi.org/10.1097/ICL.0b013e3182a3024e] [PMID: 23945525]

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