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Nanoscience & Nanotechnology-Asia

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ISSN (Print): 2210-6812
ISSN (Online): 2210-6820

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Research Article

Berberine Chloride Dihydrate Enthused Nanovesicles for the Management of Dermatitis Nanovesicles for Dermatitis

Author(s): Nimisha Srivastava, Zeeshan Fatima*, Chanchal D. Kaur and Dilshad A. Rizvi

Volume 11, Issue 3, 2021

Published on: 13 March, 2020

Page: [300 - 313] Pages: 14

DOI: 10.2174/2210681210666200313123550

Price: $65

Abstract

Background: Dermatitis is a common inflammatory skin disease that is affecting up to 25% of children and 1%-3% of adults worldwide. The paucity of the exact cure for dermatitis and untoward side effects of topical immunosuppressive steroids, has resulted in a great need for making use of complementary medicine to treat dermatitis.

Objective: The present research work involved the development of Berberine chloride dihydrate (BCD) enthused nanovesicles i.e. ethosomes for the management of dermatitis.

Methods: Ethosomes were prepared by a slight modification of cold process using varying concentrations of SPC (1-3%) and ethanol (10-40%) Optimized batch BCD 12 was further added to Carbopol 934P for gel formation. GEL BCD 12 was subjected to “anti-bacterial, dermatitis, and skin irritation study.

Results: The vesicles were in size range 142.42-398.31 nm, while the polydispersity index (PDI) ranges from 0.114-1.56, and for zeta potential, it was from-18.8 to -39.4. Entrapment efficiency was from 46.05-88.79%. Confocal laser scanning microscopy showed the penetration depth of rhodamine enthused ethosome across rat skin up to 110 μm, which was significantly higher than rhodamine solution (10 μm). In the anti-bacterial study, BCD loaded ethosomal gel (EG) showed maximum zone of inhibition of 18.5 mm against E. coli, 14.5 mm against Pseudomonas aeruginosa (P. aeruginosa) and 23.0 mm against Staphylococcus aureus (S. aureus). In dinitrochlorobenzene (DNCB) induced mice dermatitis model histopathology study showed marked decrease in the amount of inflammatory cell nucleus in mice treated with BCD loaded ethosomal gel followed by 56% and 50% increase in ear swelling and ear mass respectively in morphology study. The conventional marketed formulation showed a nominal decrease in epidermal thickness, a 66.67% increase in ear thickness, and 63.64% increase in ear mass. Further Primary irritation index was less than 0.4 indicating negligible irritation in all the groups.

Conclusion: It can be concluded that ethosomal gel is not only an efficient carrier for BCD but also proves its potential for the management of dermatitis.

Keywords: Berberine chloride dihydrate, dermatitis, ethosomes, soyaphosphatidylcholine, gel, eczema.

Graphical Abstract

[1]
Leung, D.Y.M.; Boguniewicz, M.; Howell, M.D.; Nomura, I.; Hamid, Q.A. New insights into atopic dermatitis. J. Clin. Invest., 2004, 113(5), 651-657.
[http://dx.doi.org/10.1172/JCI21060] [PMID: 14991059]
[2]
Dokmeci, E.; Herrick, C.A. The immune system and atopic dermatitis. Semin. Cutan. Med. Surg., 2008, 27(2), 138-143.
[http://dx.doi.org/10.1016/j.sder.2008.04.006] [PMID: 18620135]
[3]
Nutten, S. Atopic dermatitis: Global epidemiology and risk factors. Ann. Nutr. Metab., 2015, 66(Suppl. 1), 8-16.
[http://dx.doi.org/10.1159/000370220] [PMID: 25925336]
[4]
Shaw, T.E.; Currie, G.P.; Koudelka, C.W.; Simpson, E.L. Eczema prevalence in the United States: Data from the 2003 National Survey of Children’s Health. J. Invest. Dermatol., 2011, 131(1), 67-73.
[http://dx.doi.org/10.1038/jid.2010.251] [PMID: 20739951]
[5]
Williams, H.C. Epidemiology of atopic dermatitis. Clin. Exp. Dermatol., 2000, 25(7), 522-529.
[http://dx.doi.org/10.1046/j.1365-2230.2000.00698.x] [PMID: 11122223]
[6]
Furue, M.; Terao, H.; Rikihisa, W.; Urabe, K.; Kinukawa, N.; Nose, Y.; Koga, T. Clinical dose and adverse effects of topical steroids in daily management of atopic dermatitis. Br. J. Dermatol., 2003, 148(1), 128-133.
[http://dx.doi.org/10.1046/j.1365-2133.2003.04934.x] [PMID: 12534606]
[7]
Meena, A.K.; Bansal, P.; Kumar, S. Plants-herbal wealth as a potential source of ayurvedic drugs. Asian J. Tradit. Med., 2009, 4, 152-170.
[8]
Sarwa, K.K.; Das, P.J.; Mazumder, B. A nanovesicle topical formulation of Bhut Jolokia (hottest capsicum): A potential anti-arthritic medicine. Expert Opin. Drug Deliv., 2014, 11(5), 661-676.
[http://dx.doi.org/10.1517/17425247.2014.891581] [PMID: 24661126]
[9]
Shitan, N.; Tanaka, M.; Terai, K.; Ueda, K.; Yazaki, K. Human MDR1 and MRP1 recognize berberine as their transport substrate. Biosci. Biotechnol. Biochem., 2007, 71(1), 242-245.
[http://dx.doi.org/10.1271/bbb.60441] [PMID: 17213652]
[10]
Shuang, Guo; Guo, G.W.; Tong, Wu. Solid dispersion of berberinehydrochlorid and Eudragit® S100: Formulation, physicochemical characterization and cytotoxicity evaluation. J. Drug Deliv. Sci. Technol., 2017, 40, 21-27.
[11]
Chen, W.; Miao, Y.Q.; Fan, D.J.; Yang, S.S.; Lin, X.; Meng, L.K.; Tang, X. Bioavailability study of berberine and the enhancing effects of TPGS on intestinal absorption in rats. AAPS PharmSciTech, 2011, 12(2), 705-711.
[http://dx.doi.org/10.1208/s12249-011-9632-z] [PMID: 21637946]
[12]
Kuo, C.L.; Chi, C.W.; Liu, T.Y. The anti-inflammatory potential of berberine in vitro and in vivo. Cancer Lett., 2004, 203(2), 127-137.
[http://dx.doi.org/10.1016/j.canlet.2003.09.002] [PMID: 14732220]
[13]
Shi, C.; Tong, Q.; Fang, J.; Wang, C.; Wu, J.; Wang, W. Preparation, characterization and in vivo studies of amorphous solid dispersion of berberine with hydrogenated phosphatidylcholine. Eur. J. Pharm. Sci., 2015, 74, 11-17.
[14]
Janbaz, K.H.; Gilani, A.H. Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia, 2000, 71(1), 25-33.
[http://dx.doi.org/10.1016/S0367-326X(99)00098-2] [PMID: 11449466]
[15]
Kushwaha, S.K.; Kushwaha, N.; Maurya, N.; Rai, A. Role of markers in the standardization of herbal drugs: A review. Arch. Appl. Sci. Res., 2010, 2, 225-229.
[16]
Andola, H.C.; Rawal, R.; Rawat, M.; Bhatt, I.; Purohit, V.K. Analysis of berberine content using HPTLC fingerprinting of root and bark of three Himalayan berberis species. Asian J Biotechnol., 2010, 2, 239-245.
[http://dx.doi.org/10.3923/ajbkr.2010.239.245]
[17]
Malakar, J.; Sen, S.O.; Nayak, A.K.; Sen, K.K. Formulation, optimization and evaluation of transferosomal gel for transdermal insulin delivery. Saudi Pharm. J., 2012, 20, 355-363.
[http://dx.doi.org/10.1016/j.jsps.2012.02.001]
[18]
More, B.; Sakharwade, S.; Tembhurne, S.; Sakarkar, D. Evaluation for skin irritancy testing of developed formulations containing extract of Buteamono sperma for its topical application. Int. J. Toxicol. Appl. Pharmacol., 2013, 3, 10-13.
[19]
Li, G.; Fan, Y.; Fan, C.; Li, X.; Wang, X.; Li, M.; Liu, Y. Tacrolimus-loaded ethosomes: Physicochemical characterization and in vivo evaluation. European J. Pharmaceut. Biopharmaceut., 2012, 82, 49-57.
[http://dx.doi.org/10.1016/j.ejpb.2012.05.011]
[20]
Touitou, E.; Dayan, N.; Bergelson, L.; Godin, B.; Eliaz, M. Ethosomes-novel vesicular carriers for enhanced delivery: characterization and skin penetration properties. J. Control. Rel., 2000, 65(3), 403-418.
[21]
Srivastava, N.; Singh, K.; Amrit, K. Formulation and evaluation of Seabuckthorn leaf extract loaded ethosomal gel. Asian J. Pharm. Clin. Res., 2015, 316-320.
[22]
Jangdey, M.S.; Kaur, C.D.; Saraf, S. Efficacy of Concanavalin-A conjugated nanotransfersomal gel of apigenin for enhanced targeted delivery of UV induced skin malignant melanoma. Artif. Cells Nanomed. Biotechnol., 2019, 47(1), 904-916.
[http://dx.doi.org/10.1080/21691401.2019.1578784] [PMID: 30856018]
[23]
Kauser, H.; Mujeeb, M.; Ahad, A.; Moolakkadath, T.; Aqil, M.; Ahmad, A.; Akhter, M.H. Optimization of ethosomes for topical thymoquinone delivery for the treatment of skin acne. J. Drug Deliv. Sci. Technol., 2019, 49, 177-187.
[http://dx.doi.org/10.1016/j.jddst.2018.11.016]
[24]
Abdel-Messih, H.A.; Ishak, R.A.H.; Geneidi, A.S.; Mansour, S. Tailoring novel soft nano-vesicles ‘Flexosomes’ for enhanced transdermal drug delivery: Optimization, characterization and comprehensive ex vivo-in vivo evaluation. Int. J. Pharm., 2019, 560, 101-115.
[http://dx.doi.org/10.1016/j.ijpharm.2019.01.072] [PMID: 30753931]
[25]
Alam, M.S.; Ali, M.S.; Alam, N.; Siddiqui, M.R.; Shamim, M.; Safhi, M. In vivo study of clobetasol propionate loaded nanoemulsion for topical application in psoriasis and atopic dermatitis. Drug invention today, 2013, 5(1), 8-12.
[26]
Nimisha, Rizvi D. A Antipsoriatic and anti-inflammatory studies of Berberis aristata extract loaded nanovesicular gels. Pharmacog. Magazine, 2017, 13(1), S 587-S594.
[27]
Anis, K.V.; Rajeshkumar, N.V.; Kuttan, R. Inhibition of chemical carcinogenesis by berberine in rats and mice. J. Pharm. Pharmacol., 2001, 53(5), 763-768.
[http://dx.doi.org/10.1211/0022357011775901] [PMID: 11370717]
[28]
Boligon, A.A. da; Rosa, Moreira, L.; Piana, M.; de, Campos, M.M.A.; Oliveira, S.M. Topical antiedematogenic and anti-inflammatory effect of Scutiabuxifolia Reissek gel and stability study. J. Photochem. Photobiol. B, 2017, 167, 29-35.
[http://dx.doi.org/10.1016/j.jphotobiol.2016.12.026] [PMID: 28039787]
[29]
Chourasia, M.K.; Kang, L.; Chan, S.Y. Nanosized ethosomes bearing ketoprofen for improved transdermal delivery. Results Pharma Sci., 2011, 1(1), 60-67.
[http://dx.doi.org/10.1016/j.rinphs.2011.10.002] [PMID: 25755983]
[30]
Thasleem, M.; Mohd, Aqil; Abdul, A.; Syed, S.I.; Zeenat, I. Fisetin loaded binary ethosomes for management of skin cancer by dermal application on UV exposed mice. Int. J. Pharma., 2019560, 78-91. https://pubmed.ncbi.nlm.nih.gov/30742987/
[PMID: 30742987]
[31]
Fatima, Z. Nimisha.; Kaur, C.A.; review on potential of novel vesicular carriers for carrying herbal drugs in the treatment of dermatological disorders. J. Atoms Mol., 2016, 6(3), 987.
[32]
Pajaree, S.; Thanaporn, A.; Wiwat, P.; Sirirat, P. Formulation development of ethosomes containing indomethacin for transdermal delivery. J. Drug Deliv. Sci. Technol., 2019, 52, 760-768.
[http://dx.doi.org/10.1016/j.jddst.2019.05.048]
[33]
Li, G.; Fan, C.; Li, X.; Fan, Y.; Wang, X.; Li, M.; Liu, Y. Preparation and in vitro evaluation of tacrolimus-loaded ethosomes. Sci. World J., 2012a, 2012, 874053.
[http://dx.doi.org/10.1100/2012/874053] [PMID: 22629219]
[34]
Man, M.Q.; Hatano, Y.; Lee, S.H.; Man, M.; Chang, S.; Feingold, K.R.; Leung, D.Y.; Holleran, W.; Uchida, Y.; Elias, P.M. Characterization of a hapten-induced, murine model with multiple features of atopic dermatitis: Structural, immunologic, and biochemical changes following single versus multiple oxazolone challenges. J. Invest. Dermatol., 2008, 128(1), 79-86.
[http://dx.doi.org/10.1038/sj.jid.5701011] [PMID: 17671515]
[35]
Manoharan, S.; Muneeswaran, M.; Baskaran, N. Chemopreventive efficacy of berberine in 7, 12-dimethylbenz [a] anthracene (DMBA) induced skin carcinogenesis in Swiss albino mice. Int. J. Res. Pharm. Sci., 2010, 1, 521-529.
[36]
Nimisha; Fatima, Z.; Kaur, C. Formulation and performance evaluation of Berberis aristata extract loaded ethosomal gel. Asian J. Pharmaceut., 2017, 11(03), 176-183.
[37]
Nimisha, S.A.; Chaubey, R. Enhancement of dissolution rate of quercetin using solid dispersion approach: In vitro and in vivo evaluation. Nanosci. Nanotechnol. Asia, 2020, 10(3), 330-349.
[http://dx.doi.org/10.2174/2210681209666190919095128]
[38]
Paolino, D.; Lucania, G.; Mardente, D.; Alhaique, F.; Fresta, M. Ethosomes for skin delivery of ammonium glycyrrhizinate: In vitro percutaneous permeation through human skin and in vivo anti-inflammatory activity on human volunteers. J. Control. Release, 2005, 106(1-2), 99-110.
[http://dx.doi.org/10.1016/j.jconrel.2005.04.007] [PMID: 15935505]
[39]
Park, J.H.; Choi, J.Y.; Son, D.J.; Park, E.K.; Song, M.J.; Hellström, M.; Hong, J.T. Anti-inflammatory effect of titrated extract of Centella asiatica in phthalic anhydride-induced allergic dermatitis animal model. Int. J. Mol. Sci., 2017, 18(4), 738.
[http://dx.doi.org/10.3390/ijms18040738] [PMID: 28358324]
[40]
Sun, M.; Wang, S.; Zhao, L.; Zhao, H.; Yao, W.; Jin, W.; Wei, M. Suppression of 2,4-dinitrochlorobenzene-induced atopic dermatitis by extract of Bacillus Calmette-Guerin. Mol. Med. Rep., 2014, 9(2), 689-694.
[http://dx.doi.org/10.3892/mmr.2013.1865] [PMID: 24337259]
[41]
Alam, M.S.; Ali, M.S.; Alam, N.; Siddiqui, M.R.; Shamim, M.; Safhi, M. In vivo study of clobetasol propionate loaded nanoemulsion for topical application in psoriasis and atopic dermatitis. Drug Invent. Today, 2013, 5, 8-12.
[http://dx.doi.org/10.1016/j.dit.2013.02.001]
[42]
Lei, W.Yu.C.; Lin, H.; Zhou, X. Development of tacrolimus-loaded transfersomes for deeper skin penetration enhancement and therapeutic effect improvement in vivo. Asian J. Pharma. Sci., 2013, 8(6), 336-345.

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