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Protein & Peptide Letters

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ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

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Research Article

A Study on Serological Reactivity Profile of Different Antigen Preparations with Bancroftian filariasis Human Infection Sera

Author(s): Mohd Saeed, Vikas Kushwaha, Syed Mohd Faisal, Richa Verma, Irfan Ahmad, Huma Mustafa*, Magdah Ganash, Mohammad Amjad Kamal and Ghulam Md Ashraf*

Volume 27, Issue 9, 2020

Page: [841 - 850] Pages: 10

DOI: 10.2174/0929866527666200225123534

Price: $65

Abstract

Background: Lymphatic Filariasis (LF) is one of the incapacitating and mosquito-borne sicknesses that on progression may prompt a few recognizable types of clutters like extreme lymphedema, hydrocele, and elephantiasis.

Methods: Antigenic preparations of B. malayi adult (BmA), S. cervi adult parasites and microfilariae (mf) total parasite extract were used to analyze the serological reactivity profile with human infectious sera collected from endemic areas of Bancroftian filariasis by performing Western blot and ELISA analysis. Sera from healthy human subjects were also included in the study to determine the variation incurred in the reactivity due to the filariasis infection. Gelelectrophoresis analysis of the crude-extract of BmA revealed seven protein bands while more than ten bands were recognized in S. cervi.

Results: our results represent a clear variation in protein patterns among the crude-antigens. ELISA results showed highest prevalence of IgG, IgM and IgG4 antibodies against all antigen preparations when recorded among microfilaraemic chronic infected patients. In both the antigenic preparations, the positive reactions were in the order of microfilaraemic>endemic normal>chronic>acute>nonendemic normal subjects. All sera of Mf+ patients were uniformly positive, while sera of both chronic and endemic normal subjects showed less reactivity.

Conclusion: In the present study, we endeavoured to establish the extent of cross-reactivity of antigens derived from animal filarial parasites such as B. malayi and S. cervi with W. bancrofti filariasis sera of human patients. Besides, we further analyzed antibody-isotype profile of IgG, IgG4 and IgM in various human infection sera of bancroftian filarial subjects reactive to heterologous parasite antigens derived from adult worms of S. cervi from bovine and B. malayi from bovine and jirds.

Keywords: Lymphatic filariasis, elephantiasis, serological reactivity, heterologous parasite antigens, lymphedema, hydrocele.

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[1]
Taylor, M.J.; Hoerauf, A.; Bockarie, M. Lymphatic filariasis and onchocerciasis. Lancet, 2010, 376(9747), 1175-1185.
[http://dx.doi.org/10.1016/S0140-6736(10)60586-7 ] [PMID: 20739055]
[2]
Saeed, M.; Al-Shammari, E.M.; Khan, S.; Alam, M.J.; Adnan, M. Monitoring and evaluation of lymphatic filariasis interventions: Current trends for diagnosis. Rev. Med. Microbiol., 2016, 27(2), 75-83.
[http://dx.doi.org/10.1097/MRM.0000000000000063]
[3]
Saeed, M.; Faisal, S.M.; Ahmad, I.; Kausar, M.A.; Alam, M.J.; Khan, S.; Mustafa, H. Status of lymphatic filariasis with progression of age and gender & eradication strategies: A survey among residents of Hardoi district of Uttar Pradesh, an endemic region of North India. Cell. Mol. Biol., 2018, 64(4), 46-51.
[http://dx.doi.org/10.14715/cmb/2018.64.4.8 ] [PMID: 29642988]
[4]
WHO. Meeting of the international task force for disease eradication, January 2014. Weekly Epidemiological Record= Relevé Épidémiologique Hebdomadaire, 2014, 89(15), 153-160.
[5]
WHO. Global programme to eliminate lymphatic filariasis: Progress report on mass drug administration in 2008. Weekly Epidemiological Record= Relevé Épidémiologique Hebdomadaire 2009, 84(42), 437-444.
[6]
Global programme to eliminate lymphatic filariasis: Progress report: (2013).https://www.who.int/lymphatic_filariasis/resources/who_wer8938/en/
[7]
Ottesen, E.A. The global programme to eliminate lymphatic filariasis. Trop. Med. Int. Health, 2000, 5(9), 591-594.
[http://dx.doi.org/10.1046/j.1365-3156.2000.00620.x ] [PMID: 11044272]
[8]
Saeed, M.; Siddiqui, S.; Bajpai, P.; Srivastava, A.K.; Mustafa, H. Amplification of Brugia malayi DNA using Hha1 primer as a tool. Open Conf. Proc. J., 2014, 5, 38-40.
[9]
Zeldenryk, L.M.; Gray, M.; Speare, R.; Gordon, S.; Melrose, W. The emerging story of disability associated with lymphatic filariasis: A critical review. PLoS Negl. Trop. Dis., 2011, 5(12)e1366
[http://dx.doi.org/10.1371/journal.pntd.0001366 ] [PMID: 22216361]
[10]
Palumbo, E. Filariasis: Diagnosis, treatment and prevention. Acta Biomed., 2008, 79(2), 106-109.
[PMID: 18788504]
[11]
Koroma, J.B.; Bangura, M.M.; Hodges, M.H.; Bah, M.S.; Zhang, Y.; Bockarie, M.J. Lymphatic filariasis mapping by immuno-chromatographic test cards and baseline microfilaria survey prior to mass drug administration in Sierra Leone. Parasit. Vectors, 2012, 5(1), 10.
[http://dx.doi.org/10.1186/1756-3305-5-10 ] [PMID: 22236419]
[12]
Saeed, M.; Adnan, M.; Khan, S.; Al-Shammari, E.; Mustafa, H. In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis. Acta Parasitol., 2016, 61(1), 113-118.
[http://dx.doi.org/10.1515/ap-2016-0015 ] [PMID: 26751881]
[13]
Saeed, M.; Baig, M.H.; Bajpai, P.; Srivastava, A.K.; Ahmad, K.; Mustafa, H. Predicted binding of certain antifilarial compounds with glutathione-S-transferase of human Filariids. Bioinformation, 2013, 9(5), 233-237.
[http://dx.doi.org/10.6026/97320630009233 ] [PMID: 23516334]
[14]
Cano, J.; Rebollo, M.P.; Golding, N.; Pullan, R.L.; Crellen, T.; Soler, A.; Kelly-Hope, L.A.; Lindsay, S.W.; Hay, S.I.; Bockarie, M.J.; Brooker, S.J. The global distribution and transmission limits of lymphatic filariasis: Past and present. Parasit. Vectors, 2014, 7(1), 466.
[http://dx.doi.org/10.1186/s13071-014-0466-x ] [PMID: 25303991]
[15]
de Souza, D.K.; Koudou, B.; Kelly-Hope, L.A.; Wilson, M.D.; Bockarie, M.J.; Boakye, D.A. Diversity and transmission competence in lymphatic filariasis vectors in West Africa, and the implications for accelerated elimination of Anopheles-transmitted filariasis. Parasit. Vectors, 2012, 5(1), 259.
[http://dx.doi.org/10.1186/1756-3305-5-259 ] [PMID: 23151383]
[16]
Lammie, P.J.; Cuenco, K.T.; Punkosdy, G.A. The pathogenesis of filarial lymphedema: Is it the worm or is it the host? Ann. N. Y. Acad. Sci., 2002, 979(1), 131-142.
[http://dx.doi.org/10.1111/j.1749-6632.2002.tb04874.x ] [PMID: 12543723]
[17]
Ottesen, E.A. Immunological aspects of lymphatic filariasis and onchocerciasis in man. Trans. R Soc. Trop. Med. Hyg.,, 1984.
[http://dx.doi.org/10.1016/0035-9203(84)90309-2]
[18]
Bahgat, M.; Saad, A.; El Shahawi, G.; Gad, A.; Ramzy, K.; Ruppel, A.; Abdel Latif, M. Cross-reaction of antigen preparations from adult and larval stages of the parasite Setaria equina with sera from infected humans with Wuchereria bancrofti. East. Mediterr. Health J., 2011, 17(8), 679-686.
[http://dx.doi.org/10.26719/2011.17.8.679]
[19]
Rathaur, S.; Rai, R.; Srikanth, E.; Srivastava, S. Setaria cervi dual specific phosphatase: Characterization and its effect on eosinophil degranulation. Parasitology, 2009, 136(8), 895-904.
[http://dx.doi.org/10.1017/S0031182009006271 ] [PMID: 19523248]
[20]
Bal, M.S.; Mandal, N.N.; Das, M.K.; Kar, S.K.; Sarangi, S.S.; Beuria, M.K. Transplacental transfer of filarial antigens from Wuchereria bancrofti-infected mothers to their offspring. Parasitology, 2010, 137(4), 669-673.
[http://dx.doi.org/10.1017/S0031182009991478 ] [PMID: 19849889]
[21]
Dafa’alla, T.H.; Ghalib, H.W.; Abdelmageed, A.; Williams, J.F. The profile of IgG and IgG subclasses of onchocerciasis patients. Clin. Exp. Immunol., 1992, 88(2), 258-263.
[http://dx.doi.org/10.1111/j.1365-2249.1992.tb03070.x ] [PMID: 1572089]
[22]
Adjobimey, T.; Hoerauf, A. Induction of immunoglobulin G4 in human filariasis: An indicator of immunoregulation. Ann. Trop. Med. Parasitol., 2010, 104(6), 455-464.
[http://dx.doi.org/10.1179/136485910X12786389891407 ] [PMID: 20863434]
[23]
Babu, S.; Bhat, S.Q.; Pavan Kumar, N.; Lipira, A.B.; Kumar, S.; Karthik, C.; Kumaraswami, V.; Nutman, T.B. Filarial lymphedema is characterized by antigen-specific Th1 and th17 proinflammatory responses and a lack of regulatory T cells. PLoS Negl. Trop. Dis., 2009, 3(4)e420
[http://dx.doi.org/10.1371/journal.pntd.0000420 ] [PMID: 19381284]
[24]
Hamilton, R.G.; Hussain, R.; Ottesen, E.A.; Adkinson, N.F. Jr. The quantitation of parasite-specific human IgG and IgE in sera: Evaluation of solid-phase RIA and ELISA methodology. J. Immunol. Methods, 1981, 44(1), 101-114.
[http://dx.doi.org/10.1016/0022-1759(81)90111-3 ] [PMID: 7252171]
[25]
Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227(5259), 680-685.
[http://dx.doi.org/10.1038/227680a0 ] [PMID: 5432063]
[26]
Fatima, N.; Faisal, S.M.; Zubair, S.; Siddiqui, S.S.; Moin, S.; Owais, M. Emerging role of interleukins il-23/il-17 axis and biochemical markers in the pathogenesis of type 2 diabetes: Association with age and gender in human subjects. Int. J. Biol. Macromol., 2017, 105, 1279-1288.
[27]
Fatima, N.; Faisal, S.M.; Zubair, S.; Ajmal, M.; Siddiqui, S.S.; Moin, S.; Owais, M. Role of pro-inflammatory cytokines and biochemical markers in the pathogenesis of type 1 diabetes: Correlation with age and glycemic condition in diabetic human subjects. PLoS One, 2016, 11(8)e0161548
[http://dx.doi.org/10.1371/journal.pone.0161548 ] [PMID: 27575603]
[28]
Towbin, H.; Staehelin, T.; Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA, 1979, 76(9), 4350-4354.
[http://dx.doi.org/10.1073/pnas.76.9.4350 ] [PMID: 388439]
[29]
Mohammad, O.; Faisal, S.M.; Ahmad, N.; Rauf, M.A.; Umar, M.S.; Mujeeb, A.A.; Pachauri, P.; Ahmed, A.; Kashif, M.; Ajmal, M.; Zubair, S. Bio-mediated synthesis of 5-FU based nanoparticles employing orange fruit juice: A novel drug delivery system to treat skin fibrosarcoma in model animals. Sci. Rep., 2019, 9(1), 12288.
[http://dx.doi.org/10.1038/s41598-019-48180-7 ] [PMID: 31444363]
[30]
Lammie, P.J.; Eberhard, M.L.; Lowrie, R.C. Jr. Differential humoral and cellular immunoreactivity to saline- and detergent-extracted filarial antigens. Trans. R. Soc. Trop. Med. Hyg., 1990, 84(3), 407-410.
[http://dx.doi.org/10.1016/0035-9203(90)90339-G ] [PMID: 2260176]
[31]
Simonsen, P.E.; Meyrowitsch, D.W.; Mukoko, D.A.; Pedersen, E.M.; Malecela-Lazaro, M.N.; Rwegoshora, R.T.; Ouma, J.H.; Masese, N.; Jaoko, W.G.; Michael, E. The effect of repeated half-yearly diethylcarbamazine mass treatment on Wuchereria bancrofti infection and transmission in two East African communities with different levels of endemicity. Am. J. Trop. Med. Hyg., 2004, 70(1), 63-71.
[http://dx.doi.org/10.4269/ajtmh.2004.70.63 ] [PMID: 14971700]
[32]
Ramzy, R.M.; Gad, A.M.; Faris, R.; Weil, G.J. Evaluation of a monoclonal-antibody based antigen assay for diagnosis of Wuchereria bancrofti infection in Egypt. Am. J. Trop. Med. Hyg., 1991, 44(6), 691-695.
[http://dx.doi.org/10.4269/ajtmh.1991.44.691 ] [PMID: 1858970]
[33]
Ravindran, B.; Satapathy, A.K.; Sahoo, P.K. Bancroftian filariasis-differential reactivity of anti-sheath antibodies in microfilariae carriers. Parasite Immunol., 1994, 16(6), 321-323.
[http://dx.doi.org/10.1111/j.1365-3024.1994.tb00355.x ] [PMID: 7970869]
[34]
Simonsen, P.E.; Meyrowitsch, D.W.; Jaoko, W.G.; Malecela, M.N.; Michael, E. Immunoepidemiology of Wuchereria bancrofti infection in two East African communities: Antibodies to the microfilarial sheath and their role in regulating host microfilaraemia. Acta Trop., 2008, 106(3), 200-206.
[http://dx.doi.org/10.1016/j.actatropica.2008.03.008 ] [PMID: 18452886]
[35]
Simonsen, P.E.; Meyrowitsch, D.W.; Mukoko, D.A.; Pedersen, E.M.; Malecela-Lazaro, M.N.; Rwegoshora, R.T.; Ouma, J.H.; Masese, N.; Jaoko, W.G.; Michael, E. The effect of repeated half-yearly diethylcarbamazine mass treatment on Wuchereria bancrofti infection and transmission in two East African communities with different levels of endemicity. Am. J. Trop. Med. Hyg., 2004, 70(1), 63-71.
[http://dx.doi.org/10.4269/ajtmh.2004.70.63 ] [PMID: 14971700]
[36]
Forsyth, K.P.; Spark, R.; Kazura, J.; Brown, G.V.; Peters, P.; Heywood, P.; Dissanayake, S.; Mitchell, G.F. A monoclonal antibody-based immunoradiometric assay for detection of circulating antigen in Bancroftian filariasis. J. Immunol., 1985, 134(2), 1172-1177.
[PMID: 2578153]
[37]
Dasgupta, A.; Bala, S.; Dutta, S.N. Lymphatic filariasis in man: Demonstration of circulating antigens in Wuchereria bancrofti infection. Parasite Immunol., 1984, 6(4), 341-348.
[http://dx.doi.org/10.1111/j.1365-3024.1984.tb00806.x ] [PMID: 6433305]
[38]
Kazura, J.W.; Cicirello, H.; Forsyth, K. Differential recognition of a protective filarial antigen by antibodies from humans with Bancroftian filariasis. J. Clin. Invest., 1986, 77(6), 1985-1992.
[http://dx.doi.org/10.1172/JCI112527 ] [PMID: 2423560]

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