Abstract
Background: Seeds of Cassia auriculata are reported to be used for varying medicinal purposes; however, information on nutritive value and physical properties of seeds remains unexplored. Hence, this study is carried out to investigate the proximate, minerals, fatty acid profile, functional properties, phenolic profiling as well as antioxidant activities of seed in order to give adequate information on its suitability as a possible future food source.
Methods: The proximate compositions were evaluated by AOAC, and other standard prescribed methods. Minerals and fatty acids compositions were determined using Atomic Absorption Spectrophotometer and UV-visible spectrophotometers, while anti-nutrient determinations were carried out using standard procedures. Antioxidant activity and phenolic profiles were also analyzed using UVvisible spectrophotometers and HPLC.
Results: The results obtained from proximate analysis were: moisture content (9.74%), ash content (4.95%), protein (23.83%), lipid (6.68%), crude fiber (8.93%), total soluble sugars (53.4%) and energy value (412.6 Kcal/100g). The mineral analysis showed that nitrogen, potassium and phosphorus were the predominant elements present in the seeds. Calcium, zinc, sodium, magnesium, copper, and iron were also detected in appreciable amounts. The analyzed anti-nutritional factors were oxalate (1.38 μg/mg), phytate (0.12 μ g/mg), tannin (3.02 μg/mg), phenol (1.11 μg/mg) and saponins (0.0037%). In the seeds oils, palmitic acid (14.01%) was prominent saturated fatty acid followed by stearic acid (5.12%) and arachidic acid (2.17%), while oleic acid (21.31%) and linoleic acid (51.52%) constituted the dominant unsaturated fatty acids. The physicochemical and functional properties revealed that the seeds flour had excellent water absorption index, swelling index, foaming capacity and foam stability. In addition to that, the flour extracts revealed the dose-dependent antioxidant properties of tested extracts of the seed flour. Moreover, the HPLC analysis unveiled four phenolic compounds namely, benzoic acid (0.72 μg/mg), cinnamic acid (0.71 μg/mg), salicylic acid (0.65 μg/mg) and vanillic acid (0.61 μg/mg).
Conclusion: The results of the present study revealed that Cassia auriculata seed flour is a good source of important nutrients such as fat, protein, fibre and minerals along with edible phenolics. So the seeds would be a valuable source of dietary supplements especially for growing population and could be used for industrial and pharmaceutical purposes.
Keywords: Antioxidants, Cassia auriculata seeds, fatty acids, nutritional, phenolics, techno-functional property.
Graphical Abstract
[1]
Embaby HE, Rayan AM. Chemical composition and nutritional evaluation of the seeds of Acacia tortilis (Forssk.) Hayne ssp. raddiana. Food Chem 2016; 200: 62-8.
[http://dx.doi.org/10.1016/j.foodchem.2016.01.019 PMID: 26830561]
[http://dx.doi.org/10.1016/j.foodchem.2016.01.019 PMID: 26830561]
[2]
Falade MS, Owoyomi O, Harwood CE, et al. Chemical composition and starch hydrolysis of Acacia colei and Acacia tumida seeds. Cereal Chem 2005; 82(5): 479-84.
[http://dx.doi.org/10.1094/CC-82-0479]
[http://dx.doi.org/10.1094/CC-82-0479]
[3]
Ee KY, Yates P. Nutritional and antinutritional evaluation of raw and processed Australian wattle (Acacia saligna) seeds. Food Chem 2013; 138(2-3): 762-9.
[http://dx.doi.org/10.1016/j.foodchem.2012.10.085 PMID: 23411173]
[http://dx.doi.org/10.1016/j.foodchem.2012.10.085 PMID: 23411173]
[4]
El-Adawy TA, Khalil AH. Characteristics of roselle seeds as a new source of protein and lipid. J Agric Food Chem 1994; 42(9): 1896-900.
[http://dx.doi.org/10.1021/jf00045a013]
[http://dx.doi.org/10.1021/jf00045a013]
[5]
Embaby Hel-S. Mokhtar SM. Chemical composition and nutritive value of lantana and sweet pepper seeds and nabak seed kernels. J Food Sci 2011; 76(5): C736-41.
[http://dx.doi.org/10.1111/j.1750-3841.2011.02166.x PMID: 22417420]
[http://dx.doi.org/10.1111/j.1750-3841.2011.02166.x PMID: 22417420]
[6]
Mahmoudia M, Boughallebb F, Bouhamdac T, et al. Unexploited Polygonum equisetiforme seeds: potential source of useful natural bioactive products. Ind Crops Prod 2018; 122: 349-57.
[http://dx.doi.org/10.1016/j.indcrop.2018.06.017]
[http://dx.doi.org/10.1016/j.indcrop.2018.06.017]
[7]
El-Hashash MM, Abdel-Gawad MM, El-Sayed MM, Sabry WA, Abdel-Hameed SS. Abdel-Lateef Eel-S. Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species. Acta Pharm 2010; 60(3): 361-7.
[http://dx.doi.org/10.2478/v10007-010-0030-y] [PMID: 21134869]
[http://dx.doi.org/10.2478/v10007-010-0030-y] [PMID: 21134869]
[8]
Khyade MS, Kamble SP, Kurhe AR, et al. Comparative FTIR analysis and free radical quenching properties of three Cassia species. Asian J Pharm Clinical Res 2015; 8(5): 120-6.
[9]
Anonymous . The Wealth of India. Raw Materials, Vol II. Council of Scientific and Industrial Research: Delhi 1950; 1950: 93-8.
[10]
Ganapaty S, Thomas PS, Ramana KV, et al. Review of phytochemical studies of Cassia species. J Nat Rem 2002; 2(2): 102-20.
[11]
Singh S, Singh SK, Yadav A. A review on Cassia species: pharmacological, traditional and medicinal aspects in various countries. Am J Phytomed Clin Therap 2013; 1(3): 291-312.
[12]
Shi BJ, Zhang WD, Jiang HF, et al. A new anthraquinone from seed of Cassia obtusifolia. Nat Prod Res 2016; 30(1): 35-41.
[http://dx.doi.org/10.1080/14786419.2015.1032280 PMID: 25894611]
[http://dx.doi.org/10.1080/14786419.2015.1032280 PMID: 25894611]
[13]
Samy RP, Ignacimuthu S. Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats of India. J Ethnopharmacol 2000; 69(1): 63-71.
[http://dx.doi.org/10.1016/S0378-8741(98)00156-1 PMID: 10661885]
[http://dx.doi.org/10.1016/S0378-8741(98)00156-1 PMID: 10661885]
[15]
Warrier PK, Nambiar VP. Indian medicinal plants: a compendium of 500 species. Hyderabad: Orient Longman Ltd. 1994; Vol. 2.
[16]
Pai A, Karki R. Evaluation of antidiabetic activity of Cassia auriculata Linn. seeds for alloxan induced diabetes in rats. J Pharmaceut Res Opin 2011; 1(1): 30-3.
[17]
Doshi GM, Shahare MD, Aggarwal MD, et al. Evaluation of in-vitro antioxidant methods of Cassia auriculata. Der Pharm Lett 2011; 3(3): 297-305.
[18]
Joshi PA, Pilley HH, Wadegaonkar VP, et al. Comparative assessment of antioxidant potential of Cassia auriculata (Linn.) flower, leaf and seed methanolic extracts. Int J Pharm Pharm Sci 2015; 7(9): 381-5.
[19]
Subramanian S, Uma SK, Prasath SG. Biochemical evaluation of antidiabetic, antilipidemic and antioxidant nature of Cassia auriculata seeds studied in alloxan-induced experimental diabetes in rats. Int J Pharm Sci Rev Res 2011; 11(2): 137-44.
[20]
Jain SR, Sharma SN. Hypoglycaemic drugs of Indian indigenous origin. Planta Med 1967; 15(4): 439-42.
[http://dx.doi.org/10.1055/s-0028-1100005] [PMID: 5603487]
[http://dx.doi.org/10.1055/s-0028-1100005] [PMID: 5603487]
[21]
Puranik AS, Majagi SI, Patil PA. Effect of Cassia auriculata seed extracts on blood glucose and lipids in Wistar rats. Int J Drug Dev Res 2010; 2(4): 790-8.
[22]
Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Ray C. Screening of Indian plants for biological activity. Indian J Exp Biol 1968; 6(4): 232-47.
[PMID: 5720682]
[PMID: 5720682]
[23]
Puranik AS, Halade G, Kumar S, et al. Cassia auriculata: aspects of safety pharmacology and drug interaction. Evid Based Complement Alternat Med 2011; 2011915240
[http://dx.doi.org/10.1093/ecam/nep237]] [PMID: 21785642]
[http://dx.doi.org/10.1093/ecam/nep237]] [PMID: 21785642]
[24]
Raj YJ, Peter MPJ, Joy V. Chemical compounds investigation of Cassia auriculata seeds: a potential folklore medicinal plant. Asian J Plant Sci Res 2012; 2(2): 187-92.
[25]
Zhang Y, Nakamura S, Nakashima S, et al. Chemical structures of constituents from the seeds of Cassia auriculata. Tetrahedron 2015; 71(38): 6727-32.
[http://dx.doi.org/10.1016/j.tet.2015.07.045]
[http://dx.doi.org/10.1016/j.tet.2015.07.045]
[26]
Nakamura S, Zhang Y, Nakashima S, et al. Structures of aromatic glycosides from the seeds of Cassia auriculata. Chem Pharm Bull (Tokyo) 2016; 64(7): 970-4.
[http://dx.doi.org/10.1248/cpb.c16-00198] [PMID: 27373656]
[http://dx.doi.org/10.1248/cpb.c16-00198] [PMID: 27373656]
[27]
Official methods of analysis. 15th ed. Washington, DC: Association of Official Analytical Chemists Inc. 2000.
[28]
Khyade MS, Vaikos NP. Pharmacognostical and phytochemical evaluation of leaves of Jatropha gossypifolia L. Int J Res Ayurveda Pharm 2011; 2(1): 177-80.
[29]
Yemm EW, Willis AJ. The estimation of carbohydrates in plant extracts by anthrone. Biochem J 1954; 57(3): 508-14.
[http://dx.doi.org/10.1042/bj0570508] [PMID: 13181867]
[http://dx.doi.org/10.1042/bj0570508] [PMID: 13181867]
[30]
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193(1): 265-75.
[PMID: 14907713]
[PMID: 14907713]
[31]
Chapman HD, Pratt FP. Methods of analysis for soils, plants and water. California: California University, Agriculture Division 1961.
[32]
Zasoski RJ, Burau RG. A rapid nitric–perchloric acid digestion method for multielement tissue analysis. Commun Soil Sci Plant Anal 1977; 8(5): 425-36.
[http://dx.doi.org/10.1080/00103627709366735]
[http://dx.doi.org/10.1080/00103627709366735]
[33]
Official Methods of Analysis, Association of Official Analytical Chemists, 18th ed. Gaithersburg. 2005.
[34]
Wheeler EL, Ferrel RE. Method for phytic acid determination in wheat and wheat fractions. Cereal Chem 1971; 48: 312-20.
[35]
Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. London: Chapman and Hall 1984.
[http://dx.doi.org/10.1007/978-94-009-5570-7]
[http://dx.doi.org/10.1007/978-94-009-5570-7]
[36]
Rawdkuen S, Dena M, Ketnawa S, et al. Chemical properties and nutritional factors of pressed-cake from tea and sacha inchi seeds. Food Biosci 2016; 15: 64-71.
[http://dx.doi.org/10.1016/j.fbio.2016.05.004]
[http://dx.doi.org/10.1016/j.fbio.2016.05.004]
[38]
Mallick CP, Singh MB. Plant enzymology and histoenzymology. New Delhi: Kalyani publishers 1980.
[39]
Kasote DM, Bhalerao BM, Jagtap SD, et al. Antioxidant and alpha-amylase inhibitory activity of methanol extract of Colocasia esculenta Corm. Pharmacologyonline 2011; 2: 715-21.
[40]
Van-Wijngaarden D. Modified rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal Chem 1967; 39(7): 848-9.
[http://dx.doi.org/10.1021/ac60251a031]
[http://dx.doi.org/10.1021/ac60251a031]
[41]
Anderson RA, Conway HF, Peplinski AJ. Gelatinization of corn grits by roll cooking, extrusion cooking and steaming. Starch 1970; 22(4): 130-5.
[http://dx.doi.org/10.1002/star.19700220408]
[http://dx.doi.org/10.1002/star.19700220408]
[42]
Nwosu JN. The effects of processing on the functional properties of ‘Oze’ (Bosqueia angolensis) seeds. Pak J Nutr 2010; 9(8): 781-6.
[http://dx.doi.org/10.3923/pjn.2010.781.786]
[http://dx.doi.org/10.3923/pjn.2010.781.786]
[43]
Liadakis GN, Floridis A, Tzia C, et al. Protein isolates with reduced gossypol content from screw-pressed cottonseed meal. J Agric Food Chem 1933; 41(6): 918-22.
[http://dx.doi.org/10.1021/jf00030a016]
[http://dx.doi.org/10.1021/jf00030a016]
[44]
Subramanian SS, Nagarjan S. Flavonoids of the seeds of Crotolaria retusa and Crotolaria striata. Curr Sci 1969; 38(3): 65.
[45]
Khyade MS, Waman MB. Chemical profile and antioxidant properties of Mundulea sericea. Pharmacogn J 2017; 9(2): 213-20.
[http://dx.doi.org/10.5530/pj.2017.2.36]
[http://dx.doi.org/10.5530/pj.2017.2.36]
[46]
Mahida Y, Mohan JSS. Screening of Indian plant extracts for antibacterial activity. Pharm Biol 2006; 44(8): 627-31.
[http://dx.doi.org/10.1080/13880200600897551]
[http://dx.doi.org/10.1080/13880200600897551]
[47]
Djeridane A, Yousfi M, Nadjemi B, et al. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem 2006; 97(4): 654-60.
[http://dx.doi.org/10.1016/j.foodchem.2005.04.028]
[http://dx.doi.org/10.1016/j.foodchem.2005.04.028]
[48]
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64(4): 555-9.
[http://dx.doi.org/10.1016/S0308-8146(98)00102-2]
[http://dx.doi.org/10.1016/S0308-8146(98)00102-2]
[49]
Yıldırım A, Mavi A, Kara AA. Antioxidant and antimicrobial activities of Polygonum cognatum Meissn extracts. J Sci Food Agric 2002; 83(1): 64-9.
[http://dx.doi.org/10.1002/jsfa.1288]
[http://dx.doi.org/10.1002/jsfa.1288]
[50]
Hemalatha A, Girija K, Parthiban C, et al. Antioxidant properties and total phenolic content of a marine diatom, Navicula clavata and green microalgae, Chlorella marina and Dunaliella salina. Adv Appl Sci Res 2013; 4(5): 151-7.
[51]
General methods for Analysis. Lagos: Organization of African Unity/Scientific Technical and Research Commission 1986; Vol. 2.
[52]
Salunkhe DK, Kadam SS. Handbook of World Food Legumes: Nutritional Chemistry, Processing Technology, and Utilization. Vols I-III Boca Raton, FL: CRC Press 1989.
[53]
Daniyan SY, Abalaka ME, Aransiola SA, et al. Phytochemical screening, proximate analysis and mineral composition of cassia occidentalis seed extract. Asian J Pharm Health Sci 2011; 1(3): 145-7.
[54]
Supare S, Patil M. Estimation of phytochemical components from Cassia tora and to study its larvicidal activity. Int J Pharm Sci Invent 2015; 4(6): 11-6.
[55]
Siddhwaju P, Vijayakumari K, Janardbanan K. Chemical composition and nutritional evaluation of an underexploited legume, Acacia nilotica (L.). Del Food Chem 1996; 57: 385-91.
[http://dx.doi.org/10.1016/0308-8146(95)00238-3]
[http://dx.doi.org/10.1016/0308-8146(95)00238-3]
[56]
Bello MO, Jimoh AA. Nutrients composition of seed, chemical characterization and fatty acid composition of oil of Syzygium aromaticum. Elixir Appl Chem 2012; 42: 6065-8.
[57]
WHO Guidelines for elemental concentration, trace elements in health and human nutrition. Geneva: World Health Organization. 2000.
[58]
Gemede HF, Ratta N. Antinutritional factors in plant foods: Potential health benefits and adverse effects. Int J Nutri Food Sci 2014; 3(4): 284-9.
[http://dx.doi.org/10.11648/j.ijnfs.20140304.18]
[http://dx.doi.org/10.11648/j.ijnfs.20140304.18]
[59]
Malik A, Taufeeque M, Parveen S, et al. Evaluation of fatty acid composition of the seed oil of some leguminosae species from arid zone of Rajasthan. World J Pharm Pharm Sci 2015; 4(11): 1344-51.
[60]
Hyun TK, Song SC, Song CK, Kim JS. Nutritional and nutraceutical characteristics of Sageretia theezans fruit. Yao Wu Shi Pin Fen Xi 2015; 23(4): 742-9.
[http://dx.doi.org/10.1016/j.jfda.2015.04.006] [PMID: 28911491]
[http://dx.doi.org/10.1016/j.jfda.2015.04.006] [PMID: 28911491]
[61]
Tlili N, Tir M, Benlajnef H, et al. Variation in protein and oil content and fatty acid composition of Rhus tripartitum fruits collected at different maturity stages in different locations. Ind Crops Prod 2014; 59: 197-201.
[http://dx.doi.org/10.1016/j.indcrop.2014.05.020]
[http://dx.doi.org/10.1016/j.indcrop.2014.05.020]
[62]
Shad MA, Nawaz H, Hussain M, et al. Proximate composition and functional of lotus (Nelumbo nucifera) from Punjab, Pakistan. Pak J Bot 2011; 43(2): 895-904.
[63]
Wani IA, Sogi DS, Gill BS. Physicochemical and functional properties of flours from three Black gram (Phaseolus mungo L.) cultivars. Int J Food Sci Technol 2013; 48(4): 771-7.
[http://dx.doi.org/10.1111/ijfs.12025]
[http://dx.doi.org/10.1111/ijfs.12025]
[64]
Benítez V, Cantera S, Aguilera Y, et al. Impact of germination on starch, dietary fiber and physicochemical properties in non-conventional legumes. Food Res Int 2013; 50(1): 64-9.
[http://dx.doi.org/10.1016/j.foodres.2012.09.044]
[http://dx.doi.org/10.1016/j.foodres.2012.09.044]
[65]
Berton B, Scher J, Villieras F, et al. Measurement of hydration capacity of wheat flour: Influence of composition and physical characteristics. Powder Technol 2002; 128(1-2): 326-31.
[http://dx.doi.org/10.1016/S0032-5910(02)00168-7]
[http://dx.doi.org/10.1016/S0032-5910(02)00168-7]
[66]
Du S, Jiang H, Yu X, et al. Physicochemical and functional properties of whole legume flour. Lebensm Wiss Technol 2013; 55(1): 308-13.
[http://dx.doi.org/10.1016/j.lwt.2013.06.001]
[http://dx.doi.org/10.1016/j.lwt.2013.06.001]
[67]
Sreerama YN, Sashikala VB, Pratape VM. Phenolic compounds in cowpea and horse gram flours in comparison to chickpea flour: Evaluation of their antioxidant and enzyme inhibitory properties associated with hyperglycemia and hypertension. Food Chem 2012; 133: 156-62.
[http://dx.doi.org/10.1016/j.foodchem.2012.01.011]
[http://dx.doi.org/10.1016/j.foodchem.2012.01.011]
[68]
Wall JS. Properties of protein contributing to functionality of cereal foods. Cereal Foods World 1979; 24(7): 288-92.
[69]
Kavitha B, Hemalatha G, Kanchana S, et al. Physicochemical, functional, pasting properties and nutritional composition of selected blackgram (Phaseolus mungo L.) varieties. Indian J Sci Technol 2013; 6(10): 5386-94.
[70]
Kaur M, Singh N. Relationships between selected properties of seeds, flours, and starches from different chickpea cultivars. Int J Food Prop 2010; 9(4): 597-608.
[http://dx.doi.org/10.1080/10942910600853774]
[http://dx.doi.org/10.1080/10942910600853774]
[71]
Khoudja NK, Makhlouf LB, Madani K. Antioxidant capacity of crude extracts and their solvent fractions of selected Algerian Lamiaceae. Ind Crops Prod 2014; 52: 177-82.
[http://dx.doi.org/10.1016/j.indcrop.2013.10.004]
[http://dx.doi.org/10.1016/j.indcrop.2013.10.004]
[72]
Dorman HJD, Koşar M, Kahlos K, Holm Y, Hiltunen R. Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. J Agric Food Chem 2003; 51(16): 4563-9.
[http://dx.doi.org/10.1021/jf034108k] [PMID: 14705878]
[http://dx.doi.org/10.1021/jf034108k] [PMID: 14705878]
[73]
Duan XJ, Zhang WW, Li XM, et al. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata. Food Chem 2006; 95(1): 37-43.
[http://dx.doi.org/10.1016/j.foodchem.2004.12.015]
[http://dx.doi.org/10.1016/j.foodchem.2004.12.015]
[74]
Reddy NS, Navanesan S, Sinniah SK, Wahab NA, Sim KS. Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves. BMC Complement Altern Med 2012; 12: 128.
[http://dx.doi.org/10.1186/1472-6882-12-128] [PMID: 22898370]
[http://dx.doi.org/10.1186/1472-6882-12-128] [PMID: 22898370]
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